| Title: | Stomatal penetration and temporal dynamics of ingress of two fungal isolates associated with leaf spot disease of Jasmine (Jasminum sambac L.) |
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| Authors: | ID Rivarez, Mark Paul Selda (Author)
ID Lucila, Roy Niño R. (Author)
ID Magdalita, Pablito M. (Author) |
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| Files: |  URL - Source URL, visit https://ijoabs.com/publications-of-internationaljournalofagricultureandbiologicalsciences/2017-08/
 PDF - Presentation file, download (750,04 KB)
MD5: 27DE0491341A843A90102BF4E0562383
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Jasmine (Jasminum sambac L.) is an ornamental crop grown in South and Southeast Asia for its
flowers for garlands, tea and essential oil production. However, certain fungal foliar and floral diseases
significantly reduce its yield. In this study, Colletotrichum sp.-like (cylindrical conidia) and Fusarium sp.-
like (lunate conidia) isolates causing leaf spot disease were characterized based on the ingression process
and fungal germination in planta. Four-day single-spore cultures in PDA of isolating C1 (Colletotrichum
sp.-like) and F2 (Fusarium sp.-like) were obtained. Suspensions of 108 conidia mL-1 were made and sprayed
onto young leaves of jasmine. Specimen collection was done at 2, 6, 12, 24, 36, 48, 60 and 72 h after
inoculation (hai). Formalin-acetic acid was used to clear the tissues and fungal structures are selectively
stained using lactophenol-acid fuschin. Average ingression sites (IS) were counted for each time point under
50X magnification, then IS per leaf area (cm2) was calculated. Fungal isolates remain at the surface of the
leaf until 36 h. IS of C1 and F2 almost doubled at 48 hai. However, ingression sites declined at 72 hai for
both pathogens, which either imply a progression of sub surface colonization or unsuccessful penetration.
Leaf yellowing and a few spots were observed at 48 hai for F2 and at 60 hai for C1. More severe necrotic
leaf spots with yellow halo (severity rating of 5) were seen in plants inoculated with Fusarium sp.-like
isolated than in those inoculated with Colletotrichum sp.-like isolated. Lastly, at 48 to 60 hai, it was evident
that the pathogen started to seek for stomata, which seemed to be the preferred penetration site for both
fungal pathogens. |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 31.08.2017 |
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| Year of publishing: | 2017 |
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| Number of pages: | str. 1-12 |
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| Numbering: | Vol. 1 |
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| PID: | 20.500.12556/DiRROS-19814  |
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| UDC: | 632 |
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| ISSN on article: | 2522-6584 |
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| DOI: | 10.5281/zenodo.2574399 |
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| COBISS.SI-ID: | 59975171 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 16. 4. 2021;
Besedilo v PDF obsega 12 str.;
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| Publication date in DiRROS: | 25.07.2024 |
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| Views: | 279 |
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| Downloads: | 146 |
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