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Title:Comparison between proteome and transcriptome response in potato (Solanum tuberosum L.) leaves following potato virus Y (PVY) infection
Authors:ID Stare, Tjaša (Author)
ID Stare, Katja (Author)
ID Weckwerth, Wolfram (Author)
ID Wienkoop, Stefanie (Author)
ID Gruden, Kristina (Author)
Files:URL URL - Source URL, visit http://www.mdpi.com/2227-7382/5/3/14
 
.pdf PDF - Presentation file, download (1,41 MB)
MD5: 6AE9F4A5C2EEFA74F107C07363CF9EEF
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Plant diseases caused by viral infection are affecting all major crops. Being an obligate intracellular organisms, chemical control of these pathogens is so far not applied in the field except to control the insect vectors of the viruses. Understanding of molecular responses of plant immunity is therefore economically important, guiding the enforcement of crop resistance. To disentangle complex regulatory mechanisms of the plant immune responses, understanding system as a whole is a must. However, integrating data from different molecular analysis (transcriptomics, proteomics, metabolomics, smallRNA regulation etc.) is not straightforward. We evaluated the response of potato (Solanum tuberosum L.) following the infection with potato virus Y (PVY). The response has been analyzed on two molecular levels, with microarray transcriptome analysis and mass spectroscopy-based proteomics. Within this report, we performed detailed analysis of the results on both levels and compared two different approaches for analysis of proteomic data (spectral count versus MaxQuant). To link the data on different molecular levels, each protein was mapped to the corresponding potato transcript according to StNIB paralogue grouping. Only 33% of the proteins mapped to microarray probes in a one-to-one relation and additionally many showed discordance in detected levels of proteins with corresponding transcripts. We discussed functional importance of true biological differences between both levels and showed that the reason for the discordance between transcript and protein abundance lies partly in complexity and structure of biological regulation of proteome and transcriptome and partly in technical issues contributing to it.
Keywords:proteomics, transcriptomics, potato, potato virus Y
Publication status:Published
Publication version:Version of Record
Publication date:06.07.2017
Year of publishing:2017
Number of pages:str. 1-12
Numbering:Vol. 5, issue 3
PID:20.500.12556/DiRROS-19686 New window
UDC:578.2
ISSN on article:2227-7382
DOI:10.3390/proteomes5030014 New window
COBISS.SI-ID:4368463 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 7. 7. 2017;
Publication date in DiRROS:24.07.2024
Views:541
Downloads:341
Metadata:XML DC-XML DC-RDF
:
STARE, Tjaša, STARE, Katja, WECKWERTH, Wolfram, WIENKOOP, Stefanie and GRUDEN, Kristina, 2017, Comparison between proteome and transcriptome response in potato (Solanum tuberosum L.) leaves following potato virus Y (PVY) infection. Proteomes [online]. 2017. Vol. 5, no. 3, p. 1–12. [Accessed 4 April 2025]. DOI 10.3390/proteomes5030014. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=19686
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Record is a part of a journal

Title:Proteomes
Shortened title:Proteomes
Publisher:MDPI AG
ISSN:2227-7382
COBISS.SI-ID:523354393 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0165-2015
Name:Biotehnologija in sistemska biologija rastlin
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:N4-0026-2014
Name:Priprava molekularnih postopkov za sistemsko analizo imunskega odgovora krompirja
Funder:EC - European Commission
Funding programme:Slovenian-Austrian bilateral project (2011-2012)

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Latin
Keywords:Solanum tuberosum


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