| Title: | Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice |
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| Authors: | ID Wijk, Anne-Eva van der (Author)
ID Wisniewska-Kruk, Joanna (Author)
ID Vogels, Ilse M.C. (Author)
ID Veen, Henk A. van (Author)
ID Fung Ip, Wing (Author)
ID Wel, Nicole N. van der (Author)
ID Noorden, Cornelis J. F. van (Author)
ID Schlingemann, Reinier O. (Author)
ID Klaassen, Ingeborg (Author) |
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| Files: |  URL - Source URL, visit https://faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.201801499RRR
 PDF - Presentation file, download (2,72 MB)
MD5: 8E8F77D3868E27DD82E50EE17497A8CD
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Insight into the molecular and cellular processes in blood-retinal barrier (BRB) development, including the contribution of paracellular and transcellular pathways, is still incomplete but may help to understand the inverse process of BRB loss in pathologic eye conditions. In this comprehensive observational study, we describe in detail the formation of the BRB at the molecular level in physiologic conditions, using mice from postnatal day (P)3 to P25. Our data indicate that immature blood vessels already have tight junctions at P5, before the formation of a functional BRB. Expression of the endothelial cell-specific protein plasmalemma vesicle-associated protein (PLVAP), which is known to be involved in transcellular transport and associated with BRB permeability, decreased during development and was absent when a functional barrier was formed. Moreover, we show that PLVAP deficiency causes a transient delay in retinal vascular development and changes in mRNA expression levels of endothelial permeability pathway proteins.—Van der Wijk, A.-E., Wisniewska-Kruk, J., Vogels, I. M. C., van Veen, H. A., Ip, W. F., van der Wei, N. N., van Noorden, C. J. F., Schlingemann, R. O., Klaassen, I. Expression patterns of endothelial permeability pathways in the development of the blood-retinal barrier in mice. |
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| Keywords: | tight junctions, transcellular permeability, VEGF signaling |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.04.2019 |
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| Year of publishing: | 2019 |
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| Number of pages: | str. 5320-5333 |
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| Numbering: | Vol. 33, iss. 4 |
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| PID: | 20.500.12556/DiRROS-19597  |
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| UDC: | 577 |
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| ISSN on article: | 0892-6638 |
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| DOI: | 10.1096/fj.201801499RRR |
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| COBISS.SI-ID: | 40480773 |
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| Publication date in DiRROS: | 24.07.2024 |
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| Views: | 322 |
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| Downloads: | 184 |
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