| Title: | Potato virus Y infection alters small RNA metabolism and immune response in tomato |
|---|
| Authors: | ID Prigigallo, Maria I. (Author)
ID Križnik, Maja (Author)
ID De Paola, Domenico (Author)
ID Catalano, Domenico (Author)
ID Gruden, Kristina (Author)
ID Finetti-Sialer, Mariella M. (Author)
ID Cillo, Fabrizio (Author) |
|---|
| Files: |  URL - Source URL, visit https://www.mdpi.com/1999-4915/11/12/1100
 PDF - Presentation file, download (3,84 MB)
MD5: 6FD1A0D24C9D74ACCDAD5276001ADBA5
|
|---|
| Language: | English |
|---|
| Typology: | 1.01 - Original Scientific Article |
|---|
| Organization: |  NIB - National Institute of Biology
|
|---|
| Abstract: | Potato virus Y (PVY) isolate PVYC-to induces growth reduction and foliar symptoms in tomato, but new vegetation displays symptom recovery at a later stage. In order to investigate the role of micro(mi)RNA and secondary small(s)RNA-regulated mechanisms in tomato defenses against PVY, we performed sRNA sequencing from healthy and PVYC-to infected tomato plants at 21 and 30 days post-inoculation (dpi). A total of 792 miRNA sequences were obtained, among which were 123 canonical miRNA sequences, many isomiR variants, and 30 novel miRNAs. MiRNAs were mostly overexpressed in infected vs. healthy plants, whereas only a few miRNAs were underexpressed. Increased accumulation of isomiRs was correlated with viral infection. Among miRNA targets, enriched functional categories included resistance (R) gene families, transcription and hormone factors, and RNA silencing genes. Several 22-nt miRNAs were shown to target R genes and trigger the production of 21-nt phased sRNAs (phasiRNAs). Next, 500 phasiRNA-generating loci were identified, and were shown to be mostly active in PVY-infected tissues and at 21 dpi. These data demonstrate that sRNA-regulated host responses, encompassing miRNA alteration, diversification within miRNA families, and phasiRNA accumulation, regulate R and disease-responsive genes. The dynamic regulation of miRNAs and secondary sRNAs over time suggests a functional role of sRNA-mediated defenses in the recovery phenotype. |
|---|
| Keywords: | RNA silencing, plant defense response, Potato virus Y, molecular plant-virus interactions, microRNA, secondary small interfering RNA, small RNA sequencing, phasiRNA |
|---|
| Publication status: | Published |
|---|
| Publication version: | Version of Record |
|---|
| Publication date: | 01.12.2019 |
|---|
| Year of publishing: | 2019 |
|---|
| Number of pages: | str. 1-27 |
|---|
| Numbering: | Vol. 11, iss. 12 |
|---|
| PID: | 20.500.12556/DiRROS-19594  |
|---|
| UDC: | 577.2 |
|---|
| ISSN on article: | 1999-4915 |
|---|
| DOI: | 10.3390/v11121100 |
|---|
| COBISS.SI-ID: | 5253199 |
|---|
| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 19. 12. 2019;
|
|---|
| Publication date in DiRROS: | 24.07.2024 |
|---|
| Views: | 930 |
|---|
| Downloads: | 270 |
|---|
| Metadata: |    |
|---|
|
:
|
Copy citation |
|---|
| | | | Share: |  |
|---|
Hover the mouse pointer over a document title to show the abstract or click
on the title to get all document metadata. |
