| Title: | Structural basis for the multitasking nature of the potato virus Y coat protein |
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| Authors: | ID Kežar, Andreja (Author)
ID Kavčič, Luka (Author)
ID Pólak, Martin (Author)
ID Nováček, Jiři (Author)
ID Gutiérrez-Aguirre, Ion (Author)
ID Tušek-Žnidarič, Magda (Author)
ID Coll Rius, Anna (Author)
ID Stare, Katja (Author)
ID Gruden, Kristina (Author)
ID Ravnikar, Maja (Author)
ID Pahovnik, David (Author)
ID Žagar, Ema (Author)
ID Merzel, Franci (Author)
ID Anderluh, Gregor (Author)
ID Podobnik, Marjetka (Author) |
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| Files: |  URL - Source URL, visit https://advances.sciencemag.org/content/5/7/eaaw3808
 PDF - Presentation file, download (4,43 MB)
MD5: 4C8117F2744A889CE9588E7002A748AF
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  KI - National Institute of Chemistry
 NIB - National Institute of Biology
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| Abstract: | Potato virus Y (PVY) is among the most economically important plant pathogens. Using cryoelectron microscopy, we determined the near-atomic structure of PVY’s flexuous virions, revealing a previously unknown lumenal interplay between extended carboxyl-terminal regions of the coat protein units and viral RNA. RNA–coat protein interactions are crucial for the helical configuration and stability of the virion, as revealed by the unique near-atomic structure of RNA-free virus-like particles. The structures offer the first evidence for plasticity of the coat protein’s amino- and carboxyl-terminal regions. Together with mutational analysis and in planta experiments, we show their crucial role in PVY infectivity and explain the ability of the coat protein to perform multiple biological tasks. Moreover, the high modularity of PVY virus-like particles suggests their potential as a new molecular scaffold for nanobiotechnological applications. |
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| Keywords: | plant pathogens, potato virus Y, viral RNA |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 17.07.2019 |
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| Year of publishing: | 2019 |
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| Number of pages: | str. 1-13 |
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| Numbering: | Vol. 5, no. 7 |
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| PID: | 20.500.12556/DiRROS-19584  |
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| UDC: | 577.2 |
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| ISSN on article: | 2375-2548 |
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| DOI: | 10.1126/sciadv.aaw3808 |
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| COBISS.SI-ID: | 5135183 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 30. 7. 2019;
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| Publication date in DiRROS: | 23.07.2024 |
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| Views: | 313 |
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| Downloads: | 231 |
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