| Title: | TRIM28 selective nanobody reduces glioblastoma stem cell invasion |
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| Authors: | ID Porčnik, Andrej (Author)
ID Novak, Metka (Author)
ID Breznik, Barbara (Author)
ID Majc, Bernarda (Author)
ID Hrastar, Barbara (Author)
ID Šamec, Neja (Author)
ID Zottel, Alja (Author)
ID Jovchevska, Ivana (Author)
ID Vittori, Miloš (Author)
ID Rotter, Ana (Author)
ID Komel, Radovan (Author)
ID Lah Turnšek, Tamara (Author) |
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| Files: |  URL - Source URL, visit https://www.mdpi.com/1420-3049/26/17/5141
 PDF - Presentation file, download (1,60 MB)
MD5: 8E2816BA56D977D7F1A4215CE159DCD6
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Glioblastoma (GB), is the most common and aggressive malignant primary brain tumour in adults. Intra- and inter-tumour heterogeneity, infiltrative GB cell invasion and presence of therapy-resistant GB stem cells (GSCs) represent major obstacles to favourable prognosis and poor therapy response. Identifying the biomarkers of the most aggressive tumour cells and their more efficient targeting strategies are; therefore, crucial. Recently, transcription factor TRIM28 has been identified as a GB biomarker and, in this study, we have shown high expression of TRIM28 in GB and in low grade gliomas as well as higher expression in GSCs vs. differentiated GB cells, although in both cases not significant. We demonstrated significant in vitro inhibition of GB cells and GSCs invasiveness and spread in zebrafish brains in vivo by anti-TRIM28 selective nanobody NB237. TRIM28 was also enriched in GB (tumour) core and associated with the expression of stem cell genes, but was not prognostic for overall survival. However, based on the above results, we conclude that TRIM28 nanobody NB237 offers a new opportunity as a GB therapeutic tool. |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 25.08.2021 |
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| Year of publishing: | 2021 |
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| Number of pages: | str. 1-16 |
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| Numbering: | Vol. 26, iss. 17 |
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| PID: | 20.500.12556/DiRROS-19497  |
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| UDC: | 620.3:620.1/.2 |
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| ISSN on article: | 1420-3049 |
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| DOI: | 10.3390/molecules26175141 |
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| COBISS.SI-ID: | 74227715 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 26. 8. 2021;
Metka Novak, Barbara Breznik, Bernarda Majc, Barbara Hrastar, Neja Šamec, Alja Zottel, Ivana Jovčevska, Miloš Vittori, Ana Rotter, Radovan Komel, Tamara Lah Turnšek;
Št. članka 26175141;
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| Publication date in DiRROS: | 19.07.2024 |
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| Views: | 268 |
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| Downloads: | 210 |
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