| Title: | Fast assay to predict multipotent mesenchymal stromal cell replicative senescence dynamics |
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| Authors: | ID Kološa, Katja (Author)
ID Leskovšek, Aleš (Author)
ID Rajar, Teja (Author)
ID Lah Turnšek, Tamara (Author) |
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| Files: |  URL - Source URL, visit https://www.future-science.com/doi/10.2144/btn-2021-0087
 PDF - Presentation file, download (1,19 MB)
MD5: 0B81386B969524176A30F380A871DE12
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | The major obstacle to the application of mesenchymal stromal cells (MSCs) in regenerative medicine is the expansion of the donor-derived cells in vitro to obtain high cell numbers in the shortest possible time. However, MSCs gradually undergo replicative senescence after a variable number of divisions that reduce their therapeutic efficacy, which needs to be determined before administration. The authors developed a fast and simple evaluation assay testing two senescence inducers, mitoxantrone (Mxt) and trichostatin A (TSA), to predict the onset of spontaneous replicative senescence of adipose-derived mesenchymal stromal cells (ASCs) and have confirmed the correlation between induced senescence and spontaneous replicative senescence in the assay using Mxt. This protocol facilitates the standardization of therapeutic ASCs and MSCs from other origins before application. |
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| Keywords: | adipose mesenchymal stromal (stem) cells (ASCs), cell longevity, induced senescence, mesenchymal stromal (stem) cells (MSCs), mitoxantrone, replicative senescence, trichostatin A |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 17.02.2022 |
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| Year of publishing: | 2022 |
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| Number of pages: | str. 1-10 |
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| Numbering: | Vol. 72, no. 3 |
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| PID: | 20.500.12556/DiRROS-19343  |
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| UDC: | 577.2 |
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| ISSN on article: | 0736-6205 |
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| DOI: | 10.2144/btn-2021-0087 |
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| COBISS.SI-ID: | 100663043 |
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| Publication date in DiRROS: | 16.07.2024 |
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| Views: | 396 |
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| Downloads: | 178 |
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