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Title:The activation of iron deficiency responses of grapevine rootstocks is dependent to the availability of the nitrogen forms
Authors:ID Khalil, Sarhan (Author)
ID Strah, Rebeka (Author)
ID Lodovici, Arianna (Author)
ID Vojta, Petr (Author)
ID De Berardinis, Federica (Author)
ID Ziegler, Jörg (Author)
ID Pompe Novak, Maruša (Author)
ID Zanin, Laura (Author)
ID Tomasi, Nicola (Author)
ID Forneck, Astrid (Author)
ID Griesser, Michaela (Author)
Files:URL URL - Source URL, visit https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-024-04906-y
 
.pdf PDF - Presentation file, download (9,52 MB)
MD5: 46548A8618BF1A29ADC89B84FE041400
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Background In viticulture, iron (Fe) chlorosis is a common abiotic stress that impairs plant development and leads to yield and quality losses. Under low availability of the metal, the applied N form (nitrate and ammonium) can play a role in promoting or mitigating Fe deficiency stresses. However, the processes involved are not clear in grapevine. Therefore, the aim of this study was to investigate the response of two grapevine rootstocks to the interaction between N forms and Fe uptake. This process was evaluated in a hydroponic experiment using two ungrafted grapevine rootstocks Fercal (Vitis berlandieri x V. vinifera) tolerant to deficiency induced Fe chlorosis and Couderc 3309 (V. riparia x V. rupestris) susceptible to deficiency induced Fe chlorosis. Results The results could differentiate Fe deficiency effects, N-forms effects, and rootstock effects. Interveinal chlorosis of young leaves appeared earlier on 3309 C from the second week of treatment with NO3 −/NH4 + (1:0)/- Fe, while Fercal leaves showed less severe symptoms after four weeks of treatment, corresponding to decreased chlorophyll concentrations lowered by 75% in 3309 C and 57% in Fercal. Ferric chelate reductase (FCR) activity was by trend enhanced under Fe deficiency in Fercal with both N combinations, whereas 3309 C showed an increase in FCR activity under Fe deficiency only with NO3 −/NH4 + (1:1) treatment. With the transcriptome analysis, Gene Ontology (GO) revealed multiple biological processes and molecular functions that were significantly regulated in grapevine rootstocks under Fe-deficient conditions, with more genes regulated in Fercal responses, especially when both forms of N were supplied. Furthermore, the expression of genes involved in the auxin and abscisic acid metabolic pathways was markedly increased by the equal supply of both forms of N under Fe deficiency conditions. In addition, changes in the expression of genes related to Fe uptake, regulation, and transport reflected the different responses of the two grapevine rootstocks to different N forms. Conclusions Results show a clear contribution of N forms to the response of the two grapevine rootstocks under Fe deficiency, highlighting the importance of providing both N forms (nitrate and ammonium) in an appropriate ratio in order to ease the rootstock responses to Fe deficiency.
Keywords:vitis, iron uptake, chlorosis, nitrate, ammonium, RNA-Seq
Publication status:Published
Publication version:Version of Record
Publication date:26.03.2024
Year of publishing:2024
Number of pages:str. 1-23
Numbering:Vol. 24, [article no.] 218
PID:20.500.12556/DiRROS-19129 New window
UDC:632
ISSN on article:1471-2229
DOI:10.1186/s12870-024-04906-y New window
COBISS.SI-ID:191557635 New window
Note:Soavtorji: Rebeka Strah, Arianna Lodovici, Petr Vojta, Federica De Berardinis, Jörg Ziegler, Maruša Pompe Novak, Laura Zanin, Nicola Tomasi, Astrid Forneck & Michaela Griesser; Nasl. z nasl. zaslona; Opis vira z dne 5. 4. 2024;
Publication date in DiRROS:19.06.2024
Views:28
Downloads:12
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Record is a part of a journal

Title:BMC plant biology
Shortened title:BMC plant biol.
Publisher:BioMed Central
ISSN:1471-2229
COBISS.SI-ID:2594324 New window

Document is financed by a project

Funder:FWF - Austrian Science Fund
Funding programme:Austrian Science Fund (FWF)
Project number:P 32986
Name:Iron induced stress signaling in grapevine rootstocks

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:BI-AT/23-24-005-2023
Name:Vpliv okoljskih sprememb na fiziologijo vinske trte: pomen podlag

Funder:Other - Other funder or multiple funders
Funding programme:Austria´s Agency for Education and Internationalisation
Project number:Sl 25/2023

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:železo, vinska trta, amonij, RNA, nitrat


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