| Title: | Digital PCR for the characterization of reference materials |
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| Authors: | ID Cleveland, Megan H. (Author)
ID He, Hua-Jun (Author)
ID Milavec, Mojca (Author)
ID Bae, Young-Kyung (Author)
ID Vallone, Peter M. (Author)
ID Huggett, Jim F. (Author) |
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| Files: |  URL - Source URL, visit https://www.sciencedirect.com/science/article/pii/S0098299724000153?via%3Dihub
 PDF - Presentation file. (773,16 KB, This file will be accessible after 14.02.2025)
MD5: CE80FD876F64302FE3D7BF3F699C72A6
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| Language: | English |
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| Typology: | 1.02 - Review Article |
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| Organization: |  NIB - National Institute of Biology
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| Abstract: | Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials. |
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| Keywords: | digital PCR, dPCR, reference materials, UV-spectroscopy |
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| Publication status: | Submitted to the publisher |
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| Publication version: | Author Accepted Manuscript |
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| Publication date: | 14.02.2024 |
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| Year of publishing: | 2024 |
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| Number of pages: | str. 1-8 |
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| Numbering: | Vol. 96, [article no.] 101256 |
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| PID: | 20.500.12556/DiRROS-19040  |
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| UDC: | 577.2 |
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| ISSN on article: | 1872-9452 |
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| DOI: | 10.1016/j.mam.2024.101256 |
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| COBISS.SI-ID: | 186484995 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 22. 2. 2024;
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| Publication date in DiRROS: | 03.06.2024 |
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| Views: | 140 |
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| Downloads: | 69 |
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