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Title:Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance
Authors:ID Corrêa, Régis L. (Author)
ID Kutnjak, Denis (Author)
ID Ambrós, Silvia (Author)
ID Bustos, Mónica (Author)
ID Elena, Santiago F. (Author)
Files:URL URL - Source URL, visit https://doi.org/10.1186/s12870-024-04866-3
 
.pdf PDF - Presentation file, download (6,66 MB)
MD5: A0C245FC96FB63CE4A7CC70C697E2164
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Background: Plant responses to a wide range of stresses are known to be regulated by epigenetic mechanisms. Path-ogen-related investigations, particularly against RNA viruses, are however scarce. It has been demonstrated that Arabi-dopsis thaliana plants defective in some members of the RNA-directed DNA methylation (RdDM) or histone modi-fication pathways presented differential susceptibility to the turnip mosaic virus. In order to identify genes directly targeted by the RdDM-related RNA Polymerase V (POLV ) complex and the histone demethylase protein JUMONJI14 (JMJ14) during infection, the transcriptomes of infected mutant and control plants were obtained and integrated with available chromatin occupancy data for various epigenetic proteins and marks. Results: A comprehensive list of virus-responsive gene candidates to be regulated by the two proteins was obtained. Twelve genes were selected for further characterization, confirming their dynamic regulation during the course of infection. Several epigenetic marks on their promoter sequences were found using in silico data, raising confidence that the identified genes are actually regulated by epigenetic mechanisms. The altered expression of six of these genes in mutants of the methyltransferase gene CURLY LEAF and the histone deacetylase gene HISTONE DEACETYLASE 19 suggests that some virus-responsive genes may be regulated by multiple coordinated epigenetic complexes. A temporally separated multiple plant virus infection experiment in which plants were transiently infected with one virus and then infected by a second one was designed to investigate the possible roles of the identified POLV- and JMJ14-regulated genes in wild-type (WT ) plants. Plants that had previously been stimulated with viruses were found to be more resistant to subsequent virus challenge than control plants. Several POLV- and JMJ14-regulated genes were found to be regulated in virus induced resistance in WT plants, with some of them poisoned to be expressed in early infection stages. Conclusions: A set of confident candidate genes directly regulated by the POLV and JMJ14 proteins during virus infection was identified, with indications that some of them may be regulated by multiple epigenetic modules. A sub-set of these genes may also play a role in the tolerance of WT plants to repeated, intermittent virus infections.Keywords Biotic stress, Defense priming, Epigenetics, Histone modifications, Induced resistance, Potyvirus, RNA-directed DNA methylation.
Keywords:biotic stress, defense priming, epigenetics, histone modifications, induced resistance, Potyvirus, RNA-directed DNA methylation
Publication status:Published
Publication version:Version of Record
Publication date:05.03.2024
Year of publishing:2024
Number of pages:str. 1-20
Numbering:Vol. 24, art. no. ǂ172
PID:20.500.12556/DiRROS-18947 New window
UDC:632
ISSN on article:1471-2229
DOI:10.1186/s12870-024-04866-3 New window
COBISS.SI-ID:189559299 New window
Note:Soavtorji: Denis Kutnjak, Silvia Ambrós, Mónica Bustos and Santiago F. Elena; Nasl. z nasl. zaslona; Opis vira z dne 19. 3. 2024;
Publication date in DiRROS:17.05.2024
Views:79
Downloads:774
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Record is a part of a journal

Title:BMC plant biology
Shortened title:BMC plant biol.
Publisher:BioMed Central
ISSN:1471-2229
COBISS.SI-ID:2594324 New window

Document is financed by a project

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P4-0407-2019
Name:Okoljska in aplikativna virologija: virusi, prijatelji in sovražniki

Funder:ARIS - Slovenian Research and Innovation Agency
Project number:Z1-9158-2018
Name:Vloga epigenetskega vtisnjenja v časovno ločenih mnogoterih virusnih okužbah v rastlinah

Funder:Other - Other funder or multiple funders
Funding programme:Generalitat Valenciana and the Fondo Social Europeo
Acronym:CIDE-GENT/2021/030

Funder:Other - Other funder or multiple funders
Funding programme:Generalitat Valenciana and the Fondo Social Europeo
Acronym:CIPROM/2022/59

Funder:Other - Other funder or multiple funders
Funding programme:MCIN/AEI/10.13039/501100011033
Acronym:PID2022-136912NB-I00

Funder:Other - Other funder or multiple funders
Funding programme:ERDF a way of making Europe

Funder:Other - Other funder or multiple funders
Funding programme:FAPERJ grant 14/201

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:biotski stres, epigenetika, RNA, DNA, inducirana odpornost


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