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Title:Identification of plasma biomarker candidates in glioblastoma using an antibody-array-based proteomic approach
Authors:ID Zupančič, Klemen (Author)
ID Blejec, Andrej (Author)
ID Herman, Ana (Author)
ID Veber, Matija (Author)
ID Verbovšek, Urška (Author)
ID Koršič, Marjan (Author)
ID Knežević, Miomir (Author)
ID Rožman, Primož (Author)
ID Lah Turnšek, Tamara (Author)
ID Gruden, Kristina (Author)
ID Motaln, Helena (Author)
Files:URL URL - Source URL, visit http://www.degruyter.com/view/j/raon.2014.48.issue-3/raon-2014-0014/raon-2014-0014.xml?format=INT
 
.pdf PDF - Presentation file, download (620,85 KB)
MD5: FE98D8DA39F46E2921C077E5AF7490E9
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo OI - Institute of Oncology
Abstract:Background. Glioblastoma multiforme (GBM) is a brain tumour with a very high patient mortality rate, with a median survival of 47 weeks. This might be improved by the identification of novel diagnostic, prognostic and predictive therapy-response biomarkers, preferentially through the monitoring of the patient blood. The aim of this study was to define the impact of GBM in terms of alterations of the plasma protein levels in these patients. Materials and methods. We used a commercially available antibody array that includes 656 antibodies to analyse blood plasma samples from 17 healthy volunteers in comparison with 17 blood plasma samples from patients with GBM. Results. We identified 11 plasma proteins that are statistically most strongly associated with the presence of GBM. These proteins belong to three functional signalling pathways: T-cell signalling and immune responses; cell adhesion and migration; and cell-cycle control and apoptosis. Thus, we can consider this identified set of proteins as potential diagnostic biomarker candidates for GBM. In addition, a set of 16 plasma proteins were significantly associated with the overall survival of these patients with GBM. Guanine nucleotide binding protein alpha (GNAO1) was associated with both GBM presence and survival of patients with GBM. Conclusions. Antibody array analysis represents a useful tool for the screening of plasma samples for potential cancer biomarker candidates in small-scale exploratory experiments; however, clinical validation of these candidates requires their further evaluation in a larger study on an independent cohort of patients.
Keywords:glioblastoma, proteomics, biomarker
Publication status:Published
Publication version:Version of Record
Publication date:01.09.2014
Publisher:Association of Radiology and Oncology
Year of publishing:2014
Number of pages:str. 257-266, III
Numbering:Vol. 48, no. 3
Source:Ljubljana
PID:20.500.12556/DiRROS-18695 New window
UDC:616.831-006-07
ISSN on article:1318-2099
DOI:10.2478/raon-2014-0014 New window
COBISS.SI-ID:31525081 New window
Copyright:by Authors
Note:Soavtorji: Andrej Blejec, Ana Herman, Matija Veber, Urska Verbovsek, Marjan Korsic, Miomir Knezevic, Primoz Rozman, Tamara Lah Turnsek, Kristina Gruden, Helena Motaln; Vzporedni stvar. nasl. in izvleček v slov. na str. III;
Publication date in DiRROS:16.04.2024
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Downloads:522
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Record is a part of a journal

Title:Radiology and oncology
Shortened title:Radiol. oncol.
Publisher:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 New window

Secondary language

Language:Slovenian
Title:Ugotavljanje možnih plazemskih bioloških označevalcev pri glioblastomu z uporabo proteomske tehnike mikromrež s protitelesi
Keywords:glioblastom, proteomika, biomarker


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