| Title: | The relationship between DNA methylation and expression of three different DNA methyltransferases in ovarian cancer |
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| Authors: | ID Cör, Andrej (Author) |
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| Files: |  PDF - Presentation file, download (322,13 KB)
MD5: 25B876682916207420610D8132133782
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  OI - Institute of Oncology
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| Abstract: | Background. DNA methylation in mammals is required for embryonic development, X chromosome inactivation and imprinting. Previous studies have shown that methylation patterns become abnormal in malignant cells and may contribute to tumorigenesis. The aim of the study was to ascertain the relationship between overall DNA methylation and the expression of DNMT1, DNMT3A and DNMT3B in ovarian cancer samples. Material and methods. DNA digestion with either methylation sensitive HpaII, or methylation insensitive MspI restriction endonuclease and quantitative reverse transcription-PCR methods were used to analyse global methylation levels and expression levels of five ovarian cancerand three normal ovarian tissue samples. Results. All five analysed cancer samples were hypomethylated. The differences of methylation levels between normal ovarian tissue and carcinoma samples were statistically significant (P<0.05). All five cancer samples showed overexpression of DNMT3A and DNMT3B, and only two ovarian tumour samples showed overexpression of DNMT1. There was no correlation between global demethylation and expression levels for the three different DNMTs.Conclusion. Genome wide hypomethylation facilitates tumor development with predisposition of cells to structural and numerical chromosomal aberrations but the paradox of the global hypomethylation observed in cancer cells and the high levels of DNMTs that arepresent in these cells still remain to be resolved. |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2000 |
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| Publisher: | Slovenian Medical Association - Slovenian Association of Radiology, Nuclear Medicine Society, Slovenian Society far Radiotherapy and Oncology, and Slovenian Cancer Society |
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| Year of publishing: | 2000 |
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| Number of pages: | str. 369-374 |
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| Numbering: | Letn. 34, št. 4 |
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| Source: | Ljubljana |
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| PID: | 20.500.12556/DiRROS-17984  |
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| UDC: | 616-006 |
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| ISSN on article: | 1318-2099 |
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| COBISS.SI-ID: | 12586969 |
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| Copyright: | by Authors |
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| Note: | BSDOCID56905;
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| Publication date in DiRROS: | 25.01.2024 |
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| Views: | 525 |
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| Downloads: | 104 |
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| Metadata: |    |
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