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Title:Chloroplast redox state changes mark cell-to-cell signaling in the hypersensitive response
Authors:ID Lukan, Tjaša (Author)
ID Županič, Anže (Author)
ID Mahkovec Povalej, Tjaša (Author)
ID Brunkard, Jacob O. (Author)
ID Kmetič, Mirjam (Author)
ID Juteršek, Mojca (Author)
ID Baebler, Špela (Author)
ID Gruden, Kristina (Author)
Files:URL URL - Source URL, visit https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.18425
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo NIB - National Institute of Biology
Abstract:Bisphenol A (BPA) is one of the most commonly used substances in the manufacture ofvarious everyday products. Growing concerns about its hazardous properties, including endocrinedisruption and genotoxicity, have led to its gradual replacement by presumably safer analogues inmanufacturing plastics. The widespread use of BPA and, more recently, its analogues has increasedtheir residues in the environment. However, our knowledge of their toxicological profiles is limitedand their combined effects are unknown. In the present study, we investigated the toxic effectscaused by single bisphenols and by the combined exposure of BPA and its two analogues, BPAP andBPC, after short (24-h) and prolonged (96-h) exposure in HepG2 spheroids. The results showed thatBPA did not reduce cell viability in HepG2 spheroids after 24-h exposure. In contrast, BPAP andBPC affected cell viability in HepG2 spheroids. Both binary mixtures (BPA/BPAP and BPA/BPC)decreased cell viability in a dose-dependent manner, but the significant difference was only observedfor the combination of BPA/BPC (both at 40μM). After 96-h exposure, none of the BPs studiedaffected cell viability in HepG2 spheroids. Only the combination of BPA/BPAP decreased cellviability in a dose-dependent manner that was significant for the combination of 4μM BPA and 4μMBPAP. None of the BPs and their binary mixtures studied affected the surface area and growth ofspheroids as measured by planimetry. In addition, all BPs and their binary mixtures studied triggeredoxidative stress, as measured by the production of reactive oxygen species and malondialdehyde,at both exposure times. Overall, the results suggest that it is important to study the effects of BPsas single compounds. It is even more important to study the effects of combined exposures, as thecombined effects may differ from those induced by single compounds.
Keywords:chloroplast redox state, hypersensitive response (HR)-conferred resistance, immune signaling, live cell imaging, Solanum tuberosum (potato), spatiotemporal analysis, stromules, virus resistance
Publication version:Version of Record
Publication date:01.01.2023
Year of publishing:2023
Number of pages:str. 548-562
Numbering:Vol. 237, iss. 2
PID:20.500.12556/DiRROS-16440 New window
UDC:57
ISSN on article:1469-8137
DOI:10.1111/nph.18425 New window
COBISS.SI-ID:121824771 New window
Note:Nasl. z nasl. zaslona; Opis vira z dne 19. 9. 2022;
Publication date in DiRROS:13.04.2023
Views:335
Downloads:112
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Record is a part of a journal

Title:The new phytologist
Shortened title:New phytol.
Publisher:Cambridge University Press.
ISSN:1469-8137
COBISS.SI-ID:517796633 New window

Document is financed by a project

Funder:ARRS - Slovenian Research Agency
Project number:P4-0165-2022
Name:Biotehnologija in sistemska biologija rastlin

Funder:ARRS - Slovenian Research Agency
Project number:J4-7636-2016
Name:Prostorsko časovna analiza hipersenzitivnega odziva krompirja na krompirjev virus Y

Funder:ARRS - Slovenian Research Agency
Project number:Z4-3217-2021
Name:Medsebojna povezanost z redoks stanjem posredovane signalizacije pri odpornosti krompirja na viruse

Funder:ARRS - Slovenian Research Agency
Project number:J4-1777-2019
Name:Odkrivanje mehanizmov učinkovitosti in specifičnosti imunske signalizacije v krompirju z inovativnim pridobivanjem podatkov in modeliranjem

Funder:EC - European Commission
Funding programme:Horizon 2020
Project number:862858
Name:Accelerated Development of multi-stress tolerAnt PoTato
Acronym:ADAPT

Funder:ARRS - Slovenian Research Agency
Funding programme:Raziskovalni projekti
Project number:N4-0199
Name:Vzpostavitev metodologij za študij imunosti krompirja na nivoju posameznih celic

Funder:Other - Other funder or multiple funders
Project number:DP5-OD023072
Name:An aminoacyl tRNA synthetase is a nitrogen sensor that activates TOR in plants

Licences

License:CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
Link:http://creativecommons.org/licenses/by-nc/4.0/
Description:A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.

Secondary language

Language:Slovenian
Keywords:redoks stanje kloroplastov, krompir, preobčutljivostni odgovor, rastlinska imunska signalizacija


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