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Title:Gene electrotransfer of proinflammatory chemokines CCL5 and CCL17 as a novel approach of modifying cytokine expression profile in the tumor microenvironment
Authors:ID Božič, Tim (Author)
ID Serša, Gregor (Author)
ID Kranjc Brezar, Simona (Author)
ID Čemažar, Maja (Author)
ID Markelc, Boštjan (Author)
Files:.pdf PDF - Presentation file, download (5,63 MB)
MD5: A0CA585BC889F595C5E4FA30953968EC
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo OI - Institute of Oncology
Abstract:The effectiveness of immunotherapy highly correlates with the degree and the type of infiltrated immune cells in the tumor tissue. Treatments based on modifying the immune cell infiltrate of the tumor microenvironment are thus gaining momentum. Therefore, the aim of our study was to investigate the effects of gene therapy with two proinflammatory chemokines CCL5 and CCL17 on inflammatory cytokine expression profile and immune cell infiltrate in two murine breast tumor models, 4T1 and E0771, and two murine colon tumor models, CT26 and MC38. In vitro, lipofection of plasmid DNA encoding CCL5 or CCL17 resulted in changes in the cytokine expression profile similar to control plasmid DNA, implying that the main driver of these changes was the entry of foreign DNA into the cell%s cytosol. In vivo, gene electrotransfer resulted in high expression levels of both Ccl5 and Ccl17 transgenes in the 4T1 and CT26 tumor models. Besides a minor increase in the survival of the treated mice, the therapy also resulted in increased expression of Cxcl9 and Ifn%, potent activators of the immune system, in CT26 tumors. However, this was not recapitulated in changes of TME, implying that a further refinement of the dosing schedule is needed.
Keywords:chemokines, cytokine expression, gene electrotransfer, CCL5
Publication status:Published
Publication version:Version of Record
Publication date:09.03.2021
Publisher:Elsevier
Year of publishing:2021
Number of pages:str. 1-107795-13-107795
Numbering:Vol. 140
PID:20.500.12556/DiRROS-15560 New window
UDC:602.6/.7
ISSN on article:1567-5394
DOI:10.1016/j.bioelechem.2021.107795 New window
COBISS.SI-ID:55465219 New window
Copyright:by Authors
Publication date in DiRROS:19.09.2022
Views:515
Downloads:143
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Record is a part of a journal

Title:Bioelectrochemistry
Publisher:Elsevier
ISSN:1567-5394
COBISS.SI-ID:2502484 New window

Document is financed by a project

Funder:ARRS - Slovenian Research Agency
Project number:J3-8202-2017
Name:Spremljanje imunskega odziva v realnem času po in situ vakcinaciji z elektrokemoterapijo in pridruženo gensko terapijo z interlevkinom-12

Funder:ARRS - Slovenian Research Agency
Project number:P3-0003-2015
Name:Razvoj in ovrednotenje novih terapij za zdravljenje malignih tumorjev

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:17.09.2022

Secondary language

Language:Slovenian
Keywords:kemokini, citokinsko izražanje, genski elektroprenos, CCL5


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