| Title: | Histology basics and cell death detection in honeybee tissue |
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| Authors: | ID Smodiš Škerl, Maja Ivana (Author) |
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| Files: |  PDF - Presentation file, download (1,31 MB)
MD5: CA99A4949036910FEEB96FC3AAC44BA5
 URL - Source URL, visit https://www.jove.com/t/64141/histology-basics-and-cell-death-detection-in-honeybee-tissue
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  KIS - Agricultural Institute of Slovenia
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| Abstract: | Honeybees (Apis mellifera L.) inside the hive (nurse workers and other hive bees)
and outside the hive (foragers) are exposed to climate and weather changes, various
pesticides, pathogens, and malnutrition, mainly entering through the mouth and
primarily affecting the digestive tracts of adult bees. To understand and prevent the
effects of such external and internal stressors on honeybees, one useful research
method is the immunohistochemical method. A basic protocol is described to prepare
the midgut (ventriculus) and hypopharyngeal glands (HPGs) of adult bees for
histological analysis. A detailed methodology is described to assess the level of cell
damage and distinguish necrosis from programmed cell death (apoptosis) as a natural
process of tissue regeneration. The results of adult honeybee treatment with oxalic
acid and pesticides (insecticide and acaricide) and the determination of cell death in
the ventriculus and HPGs are presented. The pros and cons of the methodology are
also discussed. |
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| Keywords: | cell death, honeybee, histology, environment |
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| Publication status: | Published |
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| Publication version: | Author Accepted Manuscript |
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| Number of pages: | str. 1-13 |
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| Numbering: | Art. 64141 |
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| PID: | 20.500.12556/DiRROS-15267  |
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| UDC: | 638 |
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| ISSN on article: | 1940-087X |
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| DOI: | 10.3791/64141 (2022) |
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| COBISS.SI-ID: | 113957123 |
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| Note: | Nasl. z nasl. zaslona;
Opis vira z dne 6. 7. 2022;
Članek v PDF formatu obsega 13 str.;
Članek št.: e64141; |
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| Publication date in DiRROS: | 08.07.2022 |
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| Views: | 532 |
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| Downloads: | 299 |
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