Digital repository of Slovenian research organisations

Show document
A+ | A- | SLO | ENG

Title:T2-high asthma, classified by sputum mRNA expression of IL4, IL5, and IL13, is characterized by eosinophilia and severe phenotype
Authors:Rijavec, Matija (Author)
Krumpestar, Tomaž (Author)
Škrgat, Sabina (Author)
Kern, Izidor (Author)
Korošec, Peter (Author)
Language:English
Tipology:1.01 - Original Scientific Article
Organisation:Logo UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
Abstract:Asthma is a common chronic disease, with different underlying inflammatory mechanisms. Identification of asthma endotypes, which reflect a variable response to different treatments, is important for more precise asthma management. T2 asthma is characterized by airway inflammation driven by T2 cytokines including interleukins IL-4, IL-5, and IL-13. This study aimed to determine whether induced sputum samples can be used for gene expression profiling of T2-high asthma classified by IL4, IL5, and IL13 expression. Induced sputum samples were obtained from 44 subjects, among them 36 asthmatic patients and eight controls, and mRNA expression levels of IL4, IL5, and IL13 were quantified by RT-qPCR. Overall, gene expression levels of IL4, IL5, and IL13 were significantly increased in asthmatic patients' samples compared to controls and there was a high positive correlation between expressions of all three genes. T2 gene mean was calculated by combining the expression levels of all three genes (IL4, IL5, and IL13) and according to T2 gene mean expression in controls, we set a T2-high/T2-low cutoff value. Twenty-four (67%) asthmatic patients had T2-high endotype and those patients had significantly higher eosinophil blood and sputum counts. Furthermore, T2-high endotype was characterized as a more severe, difficult-to-treat asthma, and often uncontrolled despite the use of inhaled and/or oral corticosteroids. Therefore, the majority of those patients (15 [63%] of 24) needed adjunct biological therapy to control their asthma symptoms/exacerbations. In conclusion, we found that interleukins IL4, IL5, and IL13 transcripts could be effectively detected in sputum from asthmatic patients. Implementation of T2 gene mean can be used as sputum molecular biomarker to categorize patients into T2-high endotype, characterized by eosinophilia and severe, difficult-to-treat asthma, and often with a need for biological treatment.
Keywords:asthma, gene expression, interleukin-4, interleukin-5, interleukin-13, severe asthma, endotype, IL-4, IL-5, IL-13, biologic treatment
Year of publishing:2021
Publisher:MDPI
Source:Švica
COBISS_ID:49321987 Link is opened in a new window
UDC:616-097
ISSN on article:2075-1729
OceCobissID:519982617 Link is opened in a new window
DOI:10.3390/life11020092 Link is opened in a new window
Note:Nasl. z nasl. zaslona; Soavtorji: Tomaž Krumpestar, Sabina Škrgat, Izidor Kern, Peter Korošec; Opis vira z dne 29. 1. 2021; Št. članka: 92;
Views:698
Downloads:366
Files:.pdf PDF - Presentation file, download (1,31 MB)
URL URL - Source URL, visit https://www.mdpi.com/2075-1729/11/2/92/pdf
 
Journal:Life
MDPI
 
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Rights:© 2021 by the authors.
:
  
Hover the mouse pointer over a document title to show the abstract or click on the title to get all document metadata.


Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:27.01.2021

Back