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Title:Epitope mapping of major ragweed allergen Amb a 1
Authors:ID Zahirović, Abida (Author)
ID Štrukelj, Borut (Author)
ID Korošec, Peter, Klinika Golnik (Author)
ID Lunder, Mojca (Author)
Files:.pdf PDF - Presentation file, download (607,98 KB)
MD5: DB86A19E92F768190A5619EFE6D644B0
 
URL URL - Source URL, visit https://journals.matheo.si/index.php/ACSi/article/view/4516
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
Abstract:Ragweed is a prominent cause of seasonal allergies. Thus far, information on IgE-binding sites of major allergen in ragweed pollen, Amb a 1, is very limited. A powerful experimental method to gain insights on the allergen epitopes is the selection of peptides from biological libraries that bind to anti-allergen antibodies. In this work, we aimed to map IgE epitopes of Amb a 1 using epitope-mimicking short peptides % mimotopes that were affinity-selected from phage-displayed random peptide libraries. The peptides weakly aligned with the Amb a 1 primary sequence, thus suggesting that the epitopes are conformational. When the peptides were mapped onto the surface of Amb a 1 homology model, the EpiSearch analysis predicted the location of four potential epitopic sites on surface patches centred at residues K104, S110, H214, and W312. The peptides matching to the predicted epitopes bound selectively to the IgE from pool of ragweed-allergic patients% sera and therefore represent mimetics of Amb a 1 IgE epitopes. The knowledge of IgE epitopes is a prerequisite for the rational design of molecular-based approaches to diagnosis and immunotherapy of allergic diseases.
Keywords:allergens, ragweed allergy, Amb a 1, epitope mapping, phage display, mimotopes
Publication status:Published
Publication version:Version of Record
Place of publishing:Slovenija
Publisher:Slovensko kemijsko društvo
Year of publishing:2019
Number of pages:str. 37-44
Numbering:Vol. 66, no. 1
PID:20.500.12556/DiRROS-12622 New window
UDC:542:616-056.43
ISSN on article:1580-3155
DOI:10.17344/acsi.2018.4516 New window
COBISS.SI-ID:4584049 New window
Copyright: (c) 2019 Abida Zahirović, Borut Štrukelj, Peter Korošec, Mojca Lunder
Note:Nasl. z nasl. zaslona; Opis vira z dne 13. 9. 2018;
Publication date in DiRROS:09.11.2020
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Downloads:872
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Record is a part of a journal

Title:Acta chimica slovenica
Shortened title:Acta chim. slov.
Publisher:Slovensko kemijsko društvo, =Slovenian Chemical Society
ISSN:1580-3155
COBISS.SI-ID:21870085 New window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:01.01.2019

Secondary language

Language:Slovenian
Abstract:Ambrozija je pomemben vzrok sezonskih alergij. Do sedaj imamo zelo malo informacij o IgE vezavnih mestih na glavnem alergenu iz cvetnega prahu ambrozije, Amb a 1. Afinitetna selekcija peptidov iz bioloških knjižnic z uporabo specifičnih protiteles proti alergenu je uporabna laboratorijska metoda za določevanje epitopov. V tej raziskavi smo s pomočjo mimotopov, kratkih peptidov, ki posnemajo epitope, izoliranih iz bakteriofagnih knjižnic naključnih peptidov, določali IgE epitope Amb a 1. Izbrani peptidi so se le šibko ujemali s primarnim zaporedjem Amb a 1, kar je nakazovalo, da so epitopi konformacijski. Da bi jih določili, smo izdelali homologni model tridimenzionalne strukture Amb a 1, na katerega smo prilegali izbrane peptide. S pomočjo programa EpiSearch smo identificirali štiri potencialne epitope na površini alergena okoli aminokislinskih ostankov K104, S110, H214 in W312. Peptidni mimetiki predvidenih epitopov so se specifično vezali na IgE iz zmesi serumov za ambrozijo alergičnih pacientov in tako predstavljajo mimetike IgE epitopov Amb a 1. Poznavanje IgE epitopov je predpogoj za racionalno načrtovanje molekularnih pristopov v diagnostiki in imunoterapiji alergijskih bolezni.
Keywords:alergeni, bakteriofagne knjižnice, ambrozijske alergije, mimotopi, IgE epitopi


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