| Title: | Heritable risk for severe anaphylaxis associated with increased [alpha]-tryptase-encoding germline copy number at TPSAB1 |
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| Authors: | ID Lyons, Jonathan J. (Author)
ID Chovanec, Jack (Author)
ID O'Connell, Michael P. (Author)
ID Liu, Yihui (Author)
ID Šelb, Julij, Klinika Golnik (Author)
ID Zanotti, Roberta (Author)
ID Bai, Yun (Author)
ID Kim, Jiwon (Author)
ID Le, Quang T. (Author)
ID DiMaggio, Tom (Author)
ID Rijavec, Matija, Klinika Golnik, Biotehniška fakulteta UL (Author)
ID Korošec, Peter, Klinika Golnik (Author) |
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| Files: |  URL - Source URL, visit https://www.jacionline.org/article/S0091-6749(20)31029-0/fulltext
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
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| Abstract: | Background: An elevated basal serum tryptase level is associated with severe systemic anaphylaxis, most notably caused by Hymenoptera envenomation. Although clonal mast cell disease is the culprit in some individuals, it does not fully explain this clinical association. Objective: Our aim was to determine the prevalence and associated impact of tryptase genotypes on anaphylaxis in humans. Methods: Cohorts with systemic mastocytosis (SM) and venom as well as idiopathic anaphylaxis from referral centers in Italy, Slovenia, and the United States, underwent tryptase genotyping by droplet digital PCR. Associated anaphylaxis severity (Mueller scale) was subsequently examined. Healthy volunteers and controls with nonatopic disease were recruited and tryptase was genotyped by droplet digital PCR and in silico analysis of genome sequence, respectively. The effects of pooled and recombinant human tryptases, protease activated receptor 2 agonist and antagonist peptides, and a tryptase-neutralizing mAb on human umbilical vein endothelial cell permeability were assayed using a Transwell system. Results: Hereditary [alpha]-tryptasemia (H[alpha]T)--a genetic trait caused by increased [alpha]-tryptase-encoding Tryptase-[alpha]/[beta]1 (TPSAB1) copy number resulting in elevated BST level--was common in healthy individuals (5.6% [n = 7 of 125]) and controls with nonatopic disease (5.3% [n = 21 of 398]). H[alpha]T was associated with grade IV venom anaphylaxis (relative risk = 2.0; P < .05) and more prevalent in both idiopathic anaphylaxis (n = 8 of 47; [17%; P = .006]) and SM (n = 10 of 82 [12.2%; P = .03]) relative to the controls. Among patients with SM, concomitant H[alpha]T was associated with increased risk for systemic anaphylaxis (relative risk = 9.5; P = .007). In vitro, protease-activated receptor-2-dependent vascular permeability was induced by pooled mature tryptases but not [alpha]- or [beta]-tryptase homotetramers. Conclusions: Risk for severe anaphylaxis in humans is associated with inherited differences in [alpha]-tryptase-encoding copies at TPSAB1. |
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| Keywords: | mastocytosis, venoms, hypersensitivity, anaphylaxis - diagnosis, mast cells, idiopathic anaphylaxis, mast cell activation, hereditary alpha-tryptasemia |
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| Publication status: | In print |
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| Publication version: | Author Accepted Manuscript |
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| Place of publishing: | ZDA |
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| Publisher: | Elsevier |
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| Year of publishing: | 2020 |
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| Number of pages: | str. 1-11 |
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| Numbering: | Vol. 146, iss. |
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| PID: | 20.500.12556/DiRROS-12414  |
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| UDC: | 616-097 |
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| ISSN on article: | 1097-6825 |
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| DOI: | 10.1016/j.jaci.2020.06.035 |
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| COBISS.SI-ID: | 27755011 |
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| Copyright: | © 2020 Published by Elsevier Inc. |
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| Note: | Nasl. z nasl. zaslona;
Soavtorji iz Slovenije: Julij Šelb, Matija Rijavec, Peter Korošec;
Opis vira z dne 9. 9. 2020;
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| Publication date in DiRROS: | 11.09.2020 |
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| Views: | 2533 |
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| Downloads: | 489 |
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