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Title:Correlations between vitreous cytokine levels and inflammatory cells in fibrovascular membranes of patients with proliferative diabetic retinopathy
Authors:Urbančič, Mojca (Author)
Petrovič, Danijel (Author)
Milutinović Živin, Aleksandra (Author)
Korošec, Peter (Author)
Fležar, Matjaž (Author)
Globočnik Petrovič, Mojca (Author)
Language:English
Tipology:1.01 - Original Scientific Article
Organisation:Logo UKPBAG - University Clinic of Respiratory and Allergic Diseases Golnik
Abstract:Purpose: The purpose of this study was to investigate the levels of cytokines in the vitreous, and their correlation with the density of inflammatory cells in fibrovascular membranes (FVMs) in patients with proliferative diabetic retinopathy (PDR) to evaluate intraocular inflammatory conditions with regard to disease activity.Methods: Thirty-three patients (33 eyes) with PDR requiring vitreoretinal surgery because of FVMs and tractional detachment were enrolled in the study, and compared with 20 patients (20 eyes) with macular hole (MH; control group). All patients underwent complete ophthalmological examinations before surgery. The activity of the disease was noted in patients with PDR. Samples of vitreous and blood were taken, and cytokine (MCP-1, IL-8, IL-6, VEGF, IL-1%, TNF-%, MIP-1%, MIP-1%, IL-10, and IL-12) levels were measured using cytometric bead array (CBA). Samples of FVMs were analyzed with immunohistochemical methods for the presence of inflammatory cells (CD45+, CD14+, CD3+, CD4+, CD8+, and CD19+ cells), and the numerical areal density was calculated (NA). Spearman%s correlation was used to as-sess the association between variables. The Mann%Whitney test was used to assess the differences between independent groups. The Wilcoxon signed-rank test was used for assessing differences between two related groups. A p value of less than 0.05 was considered statistically significant.Results: Patients with active PDR had statistically significantly higher levels of MCP-1 (p = 0.003), VEGF (p = 0.009), and IL-8 (p = 0.02) in the vitreous in comparison with those with inactive PDR. CD45+, CD14+, CD3+, CD4+, CD8+, and CD19+ cells were identified in FVMs for patients with PDR. Statistically significantly higher numerical areal density of T lymphocytes (CD3+, CD4+, and CD8+) was demonstrated in patients with active PDR in comparison with patients with inactive PDR. Moderate to strong correlations were found between either MCP-1 or IL-8 in the vitreous, and the numerical areal density of cells (CD45+, CD3+, CD4+, and CD8+) in the FVMs, and weaker between either MCP-1 or IL-8 in the vitreous and the numerical areal density of CD14+ cells in the FVMs.Conclusions: The correlation of cytokine (MCP-1 and IL-8) vitreous levels with the density of inflammatory cells in FVMs, and differences in cytokine levels in the vitreous between patients with active and inactive PDR, and between the vitreous and serum in PDR indicate the importance of local intraocular inflammation in patients with PDR.
Keywords:diabetic retinopathy, fibrovascular membranes, inflammatory cells
Year of publishing:2020
Publisher:Molecular Vision
Source:ZDA
COBISS_ID:21204995 Link is opened in a new window
UDC:617.7
ISSN on article:1090-0535
OceCobissID:24565465 Link is opened in a new window
Note:Soavtorji: Daniel Petrovič, Aleksandra Milutinović Živin, Peter Korošec, Matjaž Fležar, Mojca Globočnik Petrovič; Nasl. z nasl. zaslona; Opis vira z dne 11. 7. 2020;
Views:405
Downloads:100
Files:.pdf PDF - Presentation file, download (784,61 KB)
URL URL - Source URL, visit https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316633/
 
Journal:Mol. vis.
[s.n.]
 
Metadata:XML RDF-CHPDL DC-XML DC-RDF
Rights:© 2020 Molecular Vision
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License:CC BY-NC-ND 4.0, Creative Commons Attribution Non-Commercial No Derivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.
Licensing start date:26.06.2020

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