161. Poročilo o preskusu št.: LVG 2023-095 : vzorec št. 2023/00508Nikica Ogris, Špela Hočevar, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Fusarium circinatum, borov smolasti rak, bor, Pinus
Published in DiRROS: 04.09.2023; Views: 205; Downloads: 0 |
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163. Poročilo o preskusu št.: LVG 2023-082 : vzorec št. 2023/00483Nikica Ogris, Zina Devetak, Špela Hočevar, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Fusarium circinatum, borov smolasti rak, Pinus, Diplodia pinea
Published in DiRROS: 31.08.2023; Views: 236; Downloads: 0 |
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165. Candidate pathogenicity factor/effector proteins of ‘Candidatus Phytoplasma solani’ modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomesMarina Dermastia, Špela Tomaž, Rebeka Strah, Tjaša Lukan, Anna Coll Rius, Barbara Dušak, Timotej Čepin, Aleš Kladnik, Maja Zagorščak, Kristina Gruden, Maruša Pompe Novak, 2023, original scientific article Abstract: The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ‘Candidiatus Phytoplasma solani’ are unknown. Six putative pathogenicity factors/effectors from six different strains of ‘Ca. P. solani’ were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate–glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate–glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed.
Keywords: autophagosome, effector, glycolysis, pathogenicity factor, StAMP
Published in DiRROS: 24.08.2023; Views: 449; Downloads: 212
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166. Influence of particle size on compressive strength of alkali activated refractory materialsBarbara Horvat, Vilma Ducman, 2020, original scientific article Abstract: Influence of particle size on the mechanical strength of alkali activated material from waste refractory monolithic was investigated in this study. Precursor was chemically and mineralogically analysed, separated on 4 fractions and alkali activated with Na-water glass. Alkali activated materials were thoroughly investigated under SEM and XRD to evaluate the not predicted differences in mechanical strength. Influence of curing temperature and time dependence at curing temperatures on mechanical strength were investigated in the sample prepared from a fraction that caused the highest compressive strength.
Keywords: refractory materials, alkali activation, particle size, SEM, XRF, XRD, compressive strength
Published in DiRROS: 21.08.2023; Views: 356; Downloads: 249
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167. Poročilo o preskusu št.: LVG 2023-075 : vzorec št. 2023/00391Barbara Piškur, Špela Hočevar, Nikica Ogris, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Fusarium circinatum, borov smolasti rak, bor, Pinus
Published in DiRROS: 16.08.2023; Views: 214; Downloads: 0 |
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169. Poročilo o preskusu št.: LVG 2023-071 : vzorec št. 2023/00139/THTine Hauptman, Matija Kadunc, Špela Hočevar, Zina Devetak, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Dothistroma, rjavenje borovih iglic, Lecanosticta acicola, rdeča pegavost borovih iglic, bori, Pinus
Published in DiRROS: 16.08.2023; Views: 246; Downloads: 0 |
170. Poročilo o preskusu št.: LVG 2023-070 : vzorec št. 2023/00373/THTine Hauptman, Matija Kadunc, Špela Hočevar, Zina Devetak, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Dothistroma, rjavenje borovih iglic, Lecanosticta acicola, rdeča pegavost borovih iglic, bori, Pinus
Published in DiRROS: 16.08.2023; Views: 263; Downloads: 0 |
