161. Poročilo o preskusu št.: LVG 2023-090 : vzorec št. 2023/00059/THTine Hauptman, Zina Devetak, Špela Hočevar, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Pseudopityophthorus minutissimus, hrastov podlubnik, Anisandrus maiche
Published in DiRROS: 08.09.2023; Views: 213; Downloads: 0 |
162. Poročilo o preskusu št.: LVG 2023-084 : vzorec št. 2023/00066/THTine Hauptman, Zina Devetak, Špela Hočevar, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Pseudopityophthorus minutissimus, hrastov podlubnik, Xylosandrus compactus, črni vejni lesar
Published in DiRROS: 08.09.2023; Views: 230; Downloads: 0 |
163. Poročilo o preskusu št.: LVG 2023-100 : vzorec št. 2023/00534Nikica Ogris, Špela Hočevar, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Fusarium circinatum, borov smolasti rak, bor, Pinus
Published in DiRROS: 07.09.2023; Views: 203; Downloads: 0 |
164. Poročilo o preskusu št.: LVG 2023-098 : vzorec št. 2023/00510Nikica Ogris, Špela Hočevar, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Fusarium circinatum, borov smolasti rak, bor, Pinus
Published in DiRROS: 06.09.2023; Views: 225; Downloads: 0 |
165. First record of the northern spruce bark beetle, Ips duplicatus (Sahlberg, 1836), in SloveniaAndreja Kavčič, Zina Devetak, Barbara Piškur, Eva Groznik, Maarten De Groot, 2023, original scientific article Abstract: The northern spruce bark beetle, Ips duplicatus (Sahlberg), has invaded Central Europe in the past century and continues to spread southwards and westwards. It has caused damage in some parts of Europe in recent decades and poses a serious threat to spruce forests across the continent. From 2018 to 2021, we conducted intensive monitoring to determine the status of I. duplicatus in Slovenia. We used different types of traps and pheromone lures that attract I. duplicatus beetles and took bark samples from bark beetle-infested trees. Of the 26 traps used during the four years of monitoring, I. duplicatus was found in 16 traps in the central part of Slovenia in 2020. Adult beetles were caught in traps containing both I. typographus and I. duplicatus lures. The identity of this species was confirmed using morphological keys and molecular methods. One to five I. duplicatus beetles were found per trap, totaling 25 specimens. However, we did not confirm any I. duplicatus in samples taken from trees in 2021. We conclude that I. duplicatus is present in Slovenia, but our results suggest that the species is only present locally and that the population is small.
Keywords: biological invasions, forest pests, temperate forests, conifers, damage, forest pest monitoring, confirmation
Published in DiRROS: 05.09.2023; Views: 358; Downloads: 168
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166. Poročilo o preskusu št.: LVG 2023-095 : vzorec št. 2023/00508Nikica Ogris, Špela Hočevar, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Fusarium circinatum, borov smolasti rak, bor, Pinus
Published in DiRROS: 04.09.2023; Views: 210; Downloads: 0 |
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168. Poročilo o preskusu št.: LVG 2023-082 : vzorec št. 2023/00483Nikica Ogris, Zina Devetak, Špela Hočevar, Barbara Piškur, 2023, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Fusarium circinatum, borov smolasti rak, Pinus, Diplodia pinea
Published in DiRROS: 31.08.2023; Views: 243; Downloads: 0 |
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170. Candidate pathogenicity factor/effector proteins of ‘Candidatus Phytoplasma solani’ modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomesMarina Dermastia, Špela Tomaž, Rebeka Strah, Tjaša Lukan, Anna Coll Rius, Barbara Dušak, Timotej Čepin, Aleš Kladnik, Maja Zagorščak, Kristina Gruden, Maruša Pompe Novak, 2023, original scientific article Abstract: The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ‘Candidiatus Phytoplasma solani’ are unknown. Six putative pathogenicity factors/effectors from six different strains of ‘Ca. P. solani’ were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate–glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate–glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed.
Keywords: autophagosome, effector, glycolysis, pathogenicity factor, StAMP
Published in DiRROS: 24.08.2023; Views: 465; Downloads: 221
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