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1.
Cell size dynamics and viability of cells exposed to hypotonic treatment and electroporation for electrofusion optimization
Marko Ušaj, Katja Trontelj, Rosana Hudej, Maša Kandušer, Damijan Miklavčič, 2009, original scientific article

Abstract: Background. Various electrofusion parameters have to be adjusted to obtain theoptimal electrofusion efficiency. Based on published data, good electrofusion conditions can be achieved with the hypotonic treatment. However, the duration of the hypotonic treatment before electroporation and buffer hypoosmolarity have to be adjusted in order to cause cell swelling, to avoid regulatory volume decrease and to preserve cell viability. The aims of our study were to determine cell size dynamics and viability of four different cell lines in hypotonic buffer and to study the influence of the electroporation on the selected cell line in hypotonic buffer. Materials and methods. Cell size dynamics of different cell lines exposed to hypotonic buffer and electroporation were analyzed by time-resolvedcell size measurements. The viability of hypotonically treated oržand electroporated cells was determined 24 h after the experiment by a modified crystal violet (CV) viability assay. Results. In our experimental conditions the hypotonic treatment at 100 mOsm was efficient for CHO, V79 and B16-F1 cell lines. The optimal duration of the treatment was between two and five minutes. On the other hand the same hypotonic treatment did not cause cell swelling of NS1 cells. Cell swelling was also observed after electroporation of B16-F1 in isotonic buffer and it was amplified when hypotonic buffer was used. In addition, the regulatory volume decrease was successfully inhibited with electroporation. Conclusions. Cell size dynamicsin hypotonic conditions should be studied for each cell line since they differ in their sensitivity to the hypotonic treatment. The inhibition of cell regulatory volume decrease by electroporation may be beneficial in achieving higher electrofusion efficiency. (Abstract truncated at 2000 characters)
Keywords: hypotonic treatment, cell swelling, regulatory volume decrease, cell size measurements, viability, electrofusion, electroporation
Published in DiRROS: 08.03.2024; Views: 117; Downloads: 36
.pdf Full text (263,01 KB)

2.
Numerical heat transfer model for swelling intumescent coatings during heating
Andrea Lucherini, Juan P. Hidalgo, Jose L. Torero, Cristian Maluk, 2023, original scientific article

Abstract: This research study presents a heat transfer model aimed at estimating the thermal and physical response of intumescent coatings. The numerical model is inspired by the outcomes of an experimental study focused on analysing the insulating effectiveness of a commercial intumescent coating for a range of heating conditions and initial coating thickness. The model solves the one-dimensional heat conduction problem using the finite-difference Crank-Nicolson method, and it assumes that the effectiveness of intumescent coatings is mainly dependent on their ability to develop swelled porous char. The coating swelling is implemented in the model by adopting an approach based on expanding the mesh representing the physical domain in proximity to the substrate-coating interface. The model described herein offers researchers and engineers a tool to estimate the heat transfer of swelling intumescent coatings (i.e. in-depth thermal gradient). Outcomes of the analysis shown herein demonstrate that the heat conduction within intumescent coatings is governed by the physical coating swelling and the thermal conditions at the coating-substrate interface. The numerical model shows that its accuracy is highly influenced by the coating thickness ahead of the reaction zone. Consequently, the coating swelling rate plays a key role, while the thermo-physical properties of the intumescent coating have a secondary effect. According to its assumptions, the model defines a quasi-steady-state thermal problem: it is more accurate for conditions close to steady-state (e.g. high heat fluxes), but it loses accuracy for cases characterised by transient phenomena (e.g. phases prior to the onset of swelling and low heat fluxes).
Keywords: intumescent coatings, heat transfer, numerical model, swelling, fire safety
Published in DiRROS: 08.01.2024; Views: 180; Downloads: 34
.pdf Full text (1,21 MB)
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