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Abstract: Steroidal glycoalkaloids ( α-solanine and α-chaconine) are major potato toxins whose accurate quantification is often challenged by matrix effects and chromatographic selectivity. This study reports the optimisation and validation of a robust reversed-phase HPLC-DAD method for the determination of glycoalkaloids in potato tissues, combined with a systematic assessment of the environmental sustainability of the analytical workflow. Extraction conditions were optimized by integrating systematic solvent screening with a factorial experimental design to evaluate the effect of key operational variables on glycoalkaloid recovery and refine extraction performance The resulting extraction medium improved extract cleanliness and chromatographic stability. A subsequent C18 solid-phase extraction SPE step further reduced matrix effect and enhanced analytical selectivity. Chromatographic separation was achieved on a conventional C18 column using an acetonitrile-water gradient, providing baseline resolution (Rs > 1.5) of α 2 -solanine and α-chaconine with stable diode-array detection (DAD) at 205 nm. The method was validated according to ICH Q2(R2) and AOAC guidelines, demonstrating excellent linearity (R > 0.995), low limits of detection (0.462–0.767 µg/mL) and quantification (1.540–2.558 µg/mL), high precision (≤ 5% RSD), and recoveries of 90–105%. Robustness testing confirmed minimal sensitivity to routine variations in chromatographic parameters. Application to potato breeding lines demonstrated reliable quantification and revealed genotype-dependent variation in glycoalkaloid content. The environmental assessment of the analytical procedure was evaluated using Analytical Eco-scale, GAPI and AGREE metrics, demonstrating reduced solvent consumption, avoidance of strongly acidic extraction conditions, and a simplified sample preparation workflow. The proposed HPLC-DAD workflow provides a validated, high-performance, and environmentally conscious approach for routine GA quantification, suitable for food-safety monitoring, regulatory compliance and breeding programme applications.
Keywords: α-chaconine, α-solanine, analytical method, HPLC-DAD, potato tubers, environmental sustainability
Published in DiRROS: 10.06.2026; Views: 266; Downloads: 270
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Abstract: Raw supporting material for article Jasmonic acid and salicylic acid interact to determine spatial regulation of gene expression responses in potato leaf to herbivory by Colorado potato beetle and mechanical wounding.
Keywords: potato, jasmonic acid, salicylic acid, Colorado potato beetle, plant response to wounding
Published in DiRROS: 08.05.2026; Views: 311; Downloads: 442
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Keywords: hyperspectral imaging, machine learning, potato plant, potato, potato cyst nematodes
Published in DiRROS: 26.03.2026; Views: 485; Downloads: 422
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Abstract: Potato virus Y (PVY) is one of the top 10 economically most important plant viruses and responsible for major yield losses. We previously suggested the involvement of the N-terminal region of PVY coat protein (CP) in PVY spread. By constructing different N-terminal deletion mutants of the PVY N605 strain, we here show that deletions of 40 or more amino acid residues from the N-terminal region of the CP resulted in the PVY multiplication limited to primary infected cells in Nicotiana clevelandii plants. Deletion of 26 residues profoundly impaired PVY cell-to-cell movement and prevented systemic PVY spread, while deletions of 19-23 residues allowed delayed systemic PVY spread. Introduced point mutations in the identified region prevent (S21G) or delay (G20P) PVY movement. In summary, this work shows the significance of the CP N-terminus for movement of the PVY.
Keywords: potato virus Y, potato, coat proteins, viral movement, point mutations
Published in DiRROS: 18.12.2025; Views: 1232; Downloads: 653
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Abstract: We performed detailed spatiotemporal analysis of chloroplast redox response to potato virus Y (PVY) infection in resistant Ny‐1-gene-bearing potato and its transgenic counterpart with impaired SA accumulation and compromised resistance. We found that the chloroplasts are highly oxidized in the cells adjacent to the cell death zone at different stages after virus inoculation in both genotypes. This hypothesis is further supported by highly induced formation of stroma filled tubules that extend from chloroplasts (stromules) in the cells adjacent to signalling cells. This dataset s a deposit of all the raw microscopy images of the study, plus the relevant metadata in ISA-tab compliant folder structure. After receiving reviews, we have made an additional experiment using a ROS inhibitor. The raw and processed data for this is in a separate file: _S_chlROS_inhibitor.zip
Keywords: cell signalling, chloroplasts, microscopy, ISA-tab, potato virus Y, redox responses, cell death, spatiotemporal analysis
Published in DiRROS: 24.10.2025; Views: 966; Downloads: 639
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