1. Functional genomics screening in Chlamydomonas reinhardtii maps the genetic landscape of tolerance to paraquat and diuronTim Godec, Carissa Bleker, Katja Stare, Tjaša Lukan, Valentina Levak, Magda Tušek-Žnidarič, Tina Kosjek, Katarina Petra Van Midden, Marina Klemenčič, Kristina Sepčić, Maruša Kerenčič, Tina Eleršek, Urban Bren, Marko Jukič, Samo Lešnik, Anže Županič, 2026, original scientific article Abstract: Functional genomics offers a powerful, unbiased approach to elucidating the molecular mechanisms underlying the toxicity of environmental pollutants. In this study, we applied genome-wide screening in Chlamydomonas reinhardtii to investigate two classical herbicides: paraquat and diuron. Our screen successfully uncovered critical nuclear-encoded pathways that govern susceptibility. For both herbicides, we identified genes regulating the assembly and maintenance of the photosynthetic machinery, highlighting the central role of nuclear control over these chloroplast-localized targets. Beyond these target-related factors, we discovered novel nontarget-site resistance mechanisms. For paraquat, we identified intracellular trafficking as the central determinant of toxicity, experimentally characterizing a P5B ATPase transporter and a fatty acid elongation pathway whose disruption, we propose, converges on the same endomembrane delivery route through sphingolipid depletion. In contrast, our screening data suggest that diuron tolerance may be associated with a metabolic strategy focused on energy conservation, where the inactivation of specific NADPH-consuming enzymes could preserve reducing power for essential antioxidant defense. Collectively, these findings demonstrate that functional genomics can reveal novel, complex modes of action even for well-characterized chemicals, providing the mechanistic resolution required to advance modern ecotoxicological risk assessments.
Keywords: paraquat, diuron
Published in DiRROS: 09.06.2026; Views: 78; Downloads: 56
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2. Jasmonic acid and salicylic acid interact to determine spatial regulation of gene expression responses in potato leaf to herbivory by Colorado potato beetle and mechanical wounding : version v1Valentina Levak, Tjaša Mahkovec Povalej, Karmen Pogačar, Katja Stare, Maja Zagorščak, Tim Hawkins, Joanne Robson, David Dobnik, Tjaša Lukan, Kristina Gruden, 2025, complete scientific database of research data Abstract: Raw supporting material for article Jasmonic acid and salicylic acid interact to determine spatial regulation of gene expression responses in potato leaf to herbivory by Colorado potato beetle and mechanical wounding.
Keywords: potato, jasmonic acid, salicylic acid, Colorado potato beetle, plant response to wounding
Published in DiRROS: 08.05.2026; Views: 124; Downloads: 141
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3. Metadata from: Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal regionAnže Vozelj, Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek-Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutiérrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll Rius, Tjaša Lukan, 2025, complete scientific database of research data Keywords: potato virus Y, coat protein, viral movement, point mutations, Solanum tuberosum
Published in DiRROS: 31.03.2026; Views: 278; Downloads: 281
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4. Jasmonic acid and salicylic acid interact to determine spatial regulation of gene expression responses in potato leaf to herbivory by Colorado potato beetle and mechanical woundingValentina Levak, Tjaša Mahkovec Povalej, Karmen Pogačar, Katja Stare, Maja Zagorščak, Tim Hawkins, Joanne Robson, David Dobnik, Tjaša Lukan, Kristina Gruden, 2025, original scientific article Abstract: We investigated the spatial dynamics of potato (Solanum tuberosum) responses to herbivory and mechanical wounding. We first followed the spatiotemporal response of jasmonic acid (JA) signaling, known to be involved in the response. We generated two potato sensor lines: a JAZ degradation sensor and a downstream multicystatin (MC) transcriptional reporter. Both sensors revealed concentric, locally restricted responses on wounded leaves. Notably, JAdependent gene expression was absent in cells immediately adjacent to the wound, whereas JAZ degradation spread continuously outward from the wound site. This pattern occurred after both herbivore attack and mechanical injury by the needle. To probe the mechanism, a salicylic acid (SA) reporter showed SA accumulation near the wound. Introducing the MC reporter into SA-depleted NahG plants produced a uniform spread of MC expression, confirming that SA attenuates the JA response in proximal cells. Together, these results show that a locally distinct, spatiotemporal SA–JA crosstalk shapes wound responses in potato, extending principles known from pathogen–plant interactions to herbivory and mechanical damage.
Keywords: Colorado potato beetle, herbivore, hormonal crosstalk, jasmonic acid, potato, salicylic acid, wounding
Published in DiRROS: 28.01.2026; Views: 475; Downloads: 411
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5. Functionality of potato virus Y coat protein in cell-to-cell movement dynamics is defined by its N-terminal regionAnže Vozelj, Tjaša Mahkovec Povalej, Katja Stare, Magda Tušek-Žnidarič, Katarina Bačnik, Valentina Levak, Ion Gutiérrez-Aguirre, Marjetka Podobnik, Kristina Gruden, Anna Coll Rius, Tjaša Lukan, 2025, original scientific article Abstract: Potato virus Y (PVY) is one of the top 10 economically most important plant viruses and responsible for major yield losses. We previously suggested the involvement of the N-terminal region of PVY coat protein (CP) in PVY spread. By constructing different N-terminal deletion mutants of the PVY N605 strain, we here show that deletions of 40 or more amino acid residues from the N-terminal region of the CP resulted in the PVY multiplication limited to primary infected cells in Nicotiana clevelandii plants. Deletion of 26 residues profoundly impaired PVY cell-to-cell movement and prevented systemic PVY spread, while deletions of 19-23 residues allowed delayed systemic PVY spread. Introduced point mutations in the identified region prevent (S21G) or delay (G20P) PVY movement. In summary, this work shows the significance of the CP N-terminus for movement of the PVY.
Keywords: potato virus Y, potato, coat proteins, viral movement, point mutations
Published in DiRROS: 18.12.2025; Views: 1067; Downloads: 490
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6. Chloroplast redox state changes indicate cell-to-cell signalling during the hypersensitive response : version v3Tjaša Lukan, Kristina Gruden, Anže Županič, Tjaša Mahkovec Povalej, 2021, complete scientific database of research data Abstract: We performed detailed spatiotemporal analysis of chloroplast redox response to potato virus Y (PVY) infection in resistant Ny‐1-gene-bearing potato and its transgenic counterpart with impaired SA accumulation and compromised resistance. We found that the chloroplasts are highly oxidized in the cells adjacent to the cell death zone at different stages after virus inoculation in both genotypes. This hypothesis is further supported by highly induced formation of stroma filled tubules that extend from chloroplasts (stromules) in the cells adjacent to signalling cells. This dataset s a deposit of all the raw microscopy images of the study, plus the relevant metadata in ISA-tab compliant folder structure. After receiving reviews, we have made an additional experiment using a ROS inhibitor. The raw and processed data for this is in a separate file: _S_chlROS_inhibitor.zip
Keywords: cell signalling, chloroplasts, microscopy, ISA-tab, potato virus Y, redox responses, cell death, spatiotemporal analysis
Published in DiRROS: 24.10.2025; Views: 685; Downloads: 420
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8. Protein complexes from edible mushrooms as a sustainable potato protection against coleopteran pestsKarmen Pogačar, Maja Grundner, Primož Žigon, Anna Coll Rius, Anastasija Panevska, Tjaša Lukan, Marko Petek, Jaka Razinger, Kristina Gruden, Kristina Sepčić, 2024, original scientific article Keywords: biopesticides, Leptinotarsa decemlineata, Solanum tuberosum, insect stress response, larval toxicity, transgenic crop
Published in DiRROS: 14.05.2025; Views: 953; Downloads: 575
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9. Protein complexes from edible mushrooms as a sustainable potato protection against coleopteran pestsKarmen Pogačar, Maja Grundner, Primož Žigon, Anna Coll Rius, Anastasija Panevska, Tjaša Lukan, Marko Petek, Jaka Razinger, Kristina Gruden, Kristina Sepčić, 2024, original scientific article Keywords: biopesticides, Leptinotarsa decemlineata, Solanum tuberosum, insect stress response, larval toxicity, transgenic crop
Published in DiRROS: 30.01.2025; Views: 873; Downloads: 709
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10. Zakaj toliko besed o krompirju?Valentina Levak, Tjaša Lukan, 2024, popular article Keywords: krompir, zgodovina krompirja, krompirjevi škodljivci, krompirjeve bolezni, raziskave krompirja, Nacionalni inštitut za biologijo, sistemska biologija
Published in DiRROS: 02.12.2024; Views: 1061; Downloads: 928
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