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122. Outbreak of human metapneumovirus infection in zoo, SloveniaTina Uršič, Nika Lalek, Pavel Kvapil, Marjan Kastelic, Vasilij Cociancich, Irena Grmek-Košnik, Miroslav Petrovec, 2020, other scientific articles Keywords: metapneumovirus, infection diseases, zoo Published in DiRROS: 11.09.2020; Views: 1365; Downloads: 730 Full text (383,14 KB) This document has many files! More... |
123. Mutacije ret proto-onkogena v slovenski populaciji bolnikov z medularnim rakom ščitniceSara Milićević, Damijan Bergant, Tina Žagar, Barbara Perić, 2020, original scientific article Abstract: Izhodišča. Medularni rak ščitnice (MRŠ) je redek endokrini tumor, ki se v približno 25 % primerov pojavlja v družinski obliki bolezni v sklopu multiple endokrine neoplazije tipa 2, v 75 % pa je bolezen sporadična. Namen dela je bil oceniti incidenco MRŠ ter pogostost in vrsto RET-mutacij v slovenski populaciji bolnikov med letoma 1995 in 2015.Metode. Retrospektivno smo analizirali podatke Registra raka Republike Slovenije in Registra bolnikov z MRŠ Onkološkega Inštituta v Ljubljani, zbrane med letoma 1995 in 2015. V ključili smo 143 bolnikov s potrjenim MRŠ in 43 njihovih zdravih sorodnikov, ki so bili napoteni na genetsko svetovanje in testira-nje. Genomsko DNK smo izolirali iz levkocitov periferne krvi. Eksone 10, 11, 13, 14, 15 in 16 RET protoonkogena smo pomnožili z verižno reakcijo s polimerazo. Točkovne mutacije RET proto-onkogena smo odkrili z metodo SSCA in DNA-sekvenciranjem. Zaznane mutacije so bile potrjene z restrikcijskimi encimi.Rezultati. Zarodno mutacijo RET protoonkogena smo odkrili pri 51 osebah; med njimi je bilo 37 bolnikov z MRŠ, kar predsta-vlja 25,9 % vseh bolnikov z MRŠ. Odkrili smo osem različnih mutacij RET protoonkogena. Najpogosteje smo potrdili mutacijo v kodonih 634 in 618, sledili so kodoni 790, 804 in 918. Povprečna groba letna incidenčna stopnja MRŠ v Sloveniji za opazovano obdobje znaša 0,34/100.000 prebivalcev.Zaključek. Ocenjena povprečna groba letna incidenčna stopnja MRŠ v Sloveniji je bila 0,34/100.000. Poleg tega smo ugotovili, da se RET-mutacija pojavlja v naši populaciji bolnikov v 25,9 % ter da najpogosteje prizadene kodona 634 in 618. Keywords: medularni karcinom ščitnice, multipla endokrina neoplazija 2, genetsko svetovanje, incidenca Published in DiRROS: 09.09.2020; Views: 2070; Downloads: 587 Full text (152,80 KB) |
124. PCR primers comparisons for a successful Tuber spp. DNA region amplification in routine identificationsTina Unuk Nahberger, Hojka Kraigher, Tine Grebenc, 2020, original scientific article Abstract: Since late 20th century DNA sequencing became the method of choice method in precision species identification. The ITS region is one of the official fungal barcoding DNA markers, although in some cases sequencing of the ITS re-gion may, due to misidentification, mislabeling or nomen-clature errors in public databases, lead to incorrect or insuf-ficient identification, as is currently a case in the genus Tu b e r. The aim of this study was to test, which ITS primer pairs are most appropriate and optimal for Tu b e r species DNA region amplification. Thereby we (1) compared ampli-fication success for different Tu b e r species using fungal spe-cific primer pair ITS1f and ITS4 and (2) compared amplifi-cation success using different ITS primer pair combinations in amplifying DNA region an example species Tuber aesti-vum. Based on results, Tuber aestivum was one of the most reluctant Tu b e r species in this study and in most cases failed to amplify with the above primer pair. After comparing dif-ferent ITS primer pairs, we conclude that the primer pair ITS5 and ITS7 is the most appropriate primer pair for ampli-fication DNA region of T. ae stiv um as it resulted in high am-plification success from ectomycorrhizal root tips. Based on sequences, gained from public databases, we found that ITS1f and ITS6 primers have a mismatch in one base pair compared to the target sequence of Tuber aestivum, thus re-sulting in poor or no amplification success. Although prim-er pair ITS5 and ITS7 in our study was proven to be the most appropriate primer pair in amplifying DNA region Tu b e r aestivum species, further analysis about appropriateness of it for a general barcoding and identification of ectomycorrhiza in complex community samples is needed. Keywords: Tuber spp., ITS region, PCR amplification, ITS primers Published in DiRROS: 30.07.2020; Views: 1649; Downloads: 1198 Full text (6,81 MB) This document has many files! More... |
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129. Incidenca raka na Dravskem polju : geografska analiza incidence raka na Dravskem polju s poudarkom na občini Rače-Fram in okoliških naseljih : zaključno poročiloSonja Tomšič, Vesna Zadnik, Katarina Lokar, Tina Žagar, Tjaša Kovačević, Andreja KUKEC, Tanja Rejc, Jona Blatnik, 2019, final research report Keywords: onkologija, incidenca raka, register raka, zaključna poročila Published in DiRROS: 02.06.2020; Views: 2028; Downloads: 899 Link to full text This document has many files! More... |
130. Hypogeous fungi (truffles) diversity and cultivation at the upper timber lineTine Grebenc, J. Wei, Tina Unuk Nahberger, Marcelo Aloisio Sulzbacher, Sana Jabeen, Abdul N. Khalid, Mitko Karadelev, 2019, published scientific conference contribution abstract Keywords: hypogeous fungi, upper timer line, diversity, economic evaluation Published in DiRROS: 27.05.2020; Views: 1783; Downloads: 875 Full text (153,45 KB) This document has many files! More... |