1. CRISPR/Cas-mediated plant genome editing : outstanding challenges a decade after implementationTeodoro Cardi, Jana Murovec, Allah Bakhsh, Vladislava Galović, Tjaša Lukan, Kubilay Yıldırım, Milica Zlatković, Katrijn Van Laere, 2023, review article Abstract: The discovery of the CRISPR/Cas genome-editing system has revolutionized our understanding of the plant genome. CRISPR/Cas has been used for over a decade to modify plant genomes for the study of specific genes and biosynthetic pathways as well as to speed up breeding in many plant species, including both model and non-model crops. Although the CRISPR/Cas system is very efficient for genome editing, many bottlenecks and challenges slow down further improvement and applications. In this review we discuss the challenges that can occur during tissue culture, transformation, regeneration, and mutant detection. We also review the opportunities provided by new CRISPR platforms and specific applications related to gene regulation, abiotic and biotic stress response improvement, and de novo domestication of plants. Keywords: CRISPR applications, CRISPR platforms, gene regulations, mutant detection, plant regeneration Published in DiRROS: 05.08.2024; Views: 157; Downloads: 186 Full text (4,40 MB) This document has many files! More... |
2. Evaluation and metrological performance of a novel ionisation vacuum gauge suitable as reference standardKarl Jousten, Sylke Bechstein, Matthias Bernien, Frédéric Boineau, N. Bundaleski, Claus Illgen, Berthold Jenninger, Janez Šetina, Ricardo A. S. Silva, Anke Stöltzel, O. M. N. D. Teodoro, Martin Wüest, 2023, original scientific article Keywords: ionisation vacuum gauge, hot cathode, sensitivity, secondary electrons, ion induced secondary electron yield Published in DiRROS: 26.01.2024; Views: 475; Downloads: 244 Full text (3,39 MB) This document has many files! More... |
3. Diversity of phytophthora species involved in new diseases of mountain vegetation in Europe with the description of phytophthora pseudogregata sp. nov.Carlo Bregant, Giovanni Rossetto, Letizia Meli, Nicolò Sasso, Lucio Montecchio, Ana Brglez, Barbara Piškur, Nikica Ogris, Lucia Maddau, Benedetto Teodoro Linaldeddu, 2023, original scientific article Abstract: New and emerging Phytophthora-related diseases in small trees, shrubs and herbaceous plants typical of subalpine vegetation have recently been observed in Italy and Slovenia. Diseased plants showed a complex symptomatology including foliar necrosis, fruit rot, shoot blight and branch bleeding cankers. Since little information is available about the aetiology of these aerial Phytophthora diseases, from 2019 to 2022, field surveys were conducted in 54 sites to define the occurrence, distribution and impact of the Phytophthora species on mountain vegetation. A total of 360 Phytophthora isolates were obtained from 397 samples collected from 33 herbaceous and woody host species. Based on phylogenetic analysis and morphometric data, 17 Phytophthora species were identified: P. pseudosyringae (201 isolates), P. plurivora (54), P. gonapodyides (21), P. ilicis (20), P. alpina (17), P. acerina (11), P. cactorum (7), P. pseudocryptogea (6), P. cambivora (5), P. idaei (4), P. psychrophila (3), P. bilorbang (2), P. chlamydospora (2), P. hedraiandra (1), P. kelmanii (1), P. rosacearum (1) and P. syringae (1). In addition, three isolates of a new putative Phytophthora species obtained from Alnus viridis, Juniperus communis and Rhododendron ferrugineum are described here as Phytophthora pseudogregata sp. nov. Overall, the results highlighted an unexpectedly high diversity of Phytophthora species in mountain areas, with many species able to cause aerial infections due to the production of caducous sporangia. Keywords: alpine regions, emerging disease, oomycetes, foliar necrosis, ITS clade 6, phylogeny Published in DiRROS: 01.08.2023; Views: 560; Downloads: 410 Full text (6,74 MB) This document has many files! More... |
4. First report of Diplodia fraxini and Diplodia subglobosa causing canker and dieback of Fraxinus excelsior in SloveniaBenedetto Teodoro Linaldeddu, Carlo Bregant, Lucio Montecchio, Ana Brglez, Barbara Piškur, Nikica Ogris, 2022, other scientific articles Abstract: Over the last decades the vitality and productivity of European ash trees in Slovenia has been seriously impacted by the onset of canker and dieback disease symptoms on young and old trees, primarily identified as ash dieback caused by Hymenoscyphus fraxineus. Given the limited information available about the aetiology of this emerging disease, a study was carried out to isolate, identify and characterize the fungal species involved in the observed ash symptoms. Field surveys were conducted in five forest sites where 50 symptomatic branch samples were collected. All samples were inspected and used for fungal isolation. Based on morphology, colony appearance and DNA sequence data of the internal transcribed spacer region (ITS), 125 fungal colonies belonging to five species were isolated and identified. Only a few symptomatic ash samples yielded colonies of H. fraxineus, whereas Botryosphaeriaceae species were isolated with a high frequency, with Diplodia fraxini as the dominant species. A pathogenicity test proved that all isolated species were pathogenic on European ash, causing bark lesions and wood discoloration. All Botryosphaeriaceae species isolated in this study are reported for the first time on European ash in Slovenia. Keywords: emerging disease, invasive species, Botryosphaeriaceae, pathogenicity Published in DiRROS: 15.09.2022; Views: 682; Downloads: 335 Full text (851,52 KB) This document has many files! More... |
5. Electrons on a straight path : ǂa ǂnovel ionisation vacuum gauge suitable as reference standardKarl Jousten, Matthias Bernien, Frédéric Boineau, N. Bundaleski, Claus Illgen, Berthold Jenninger, Gustav Jönsson, Janez Šetina, O. M. N. D. Teodoro, Martin Vičar, 2021, original scientific article Keywords: ionisation vacuum gauge, hot cathode, sensitivity, secondary electrons, ion induced secondary electron yield Published in DiRROS: 06.05.2022; Views: 682; Downloads: 268 Link to file |
6. Novel ionisation vacuum gauge suitable as a reference standard : influence of primary electron trajectories on the operationN. Bundaleski, C. F. Adame, Matthias Bernien, Claus Illgen, Berthold Jenninger, Karl Jousten, F. Scuderi, R. A. S. Silva, A. Stöltzel, Janez Šetina, O. M. N. D. Teodoro, Tim Verbovšek, Martin Vičar, Martin Wüest, 2022, original scientific article Keywords: vacuum gauge, hot cathode, charged particle optics, secondary electron emission Published in DiRROS: 06.05.2022; Views: 746; Downloads: 272 Link to file |