81. Prehrana po delni ali popolni odstranitvi želodcaDenis Mlakar-Mastnak, Eva Justin, 2021, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: požiranje, motnje, prehranska podpora Published in DiRROS: 20.05.2022; Views: 739; Downloads: 232 Full text (1,55 MB) |
82. New records and noteworthy data of plants, algae and fungi in SE Europe and adjacent regions, 8Gordana Tomović, Marko S. Sabovljević, Marjan Niketić, Petya Boycheva, Predrag Lazarević, Eva Kabaš, Danijela Vidaković, Jelena Krizmanić, Lado Kutnar, Janez Kermavnar, Milorad Veljković, Vladan Djordjević, Boris Assyov, Fuat Bozok, Simona Strgulc-Krajšek, Žan Lobnik Cimerman, Sorin Stefanut, Constantin-Ciprian Bîrsan, Alma Hajrudinović-Bogunić, Ante Begić, 2022, original scientific article Abstract: This paper presents new records and noteworthy data on the following taxa in SE Europe and adjacent regions: diatom alga Cylindrotheca gracilis, fungi Laccaria macrocystidiata, liverworts Riccia canaliculata, Riccia cavernosa, and Riccia frostii, mosses Dicranum viride, monocots Arundo donax, Cephalanthera rubra, Epipactis palustris, and Sternbergia colchiciflora and dicots Aconitum anthora and Cephalaria pastricensis are given within SE Europe and adjacent regions. Keywords: new report, Aconitum anthora, Arundo donax, Cephalanthera rubra, Cephalaria pastricensis, Cylindrotheca gracilis, Dicranum viride, Epipactis palustris, Laccaria macrocystidiata, Riccia canaliculata, Riccia cavernosa, Riccia frostii, Sternbergia colchiciflora, SE Europe Published in DiRROS: 20.04.2022; Views: 615; Downloads: 421 Full text (196,96 KB) This document has many files! More... |
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84. Next-generation sequencing to characterize pyrazinamide resistance in Mycobacterium tuberculosis isolates from two Balkan countriesEva Sodja, Simon Koren, Nataša Toplak, Sara Truden, Marija Žolnir-Dovč, 2021, original scientific article Abstract: Objectives. Next-generation sequencing (NGS) provide a comprehensive analysis of the genetic alterations that are most commonly linked with pyrazinamide (PZA) resistance. There are no studies reporting molecular background of PZA resistance in TB isolates from Balkan Peninsula. We aimed to examine the feasibility of full-length analysis of a gene linked with PZA resistance, pncA, using Ion Torrent technology in comparison to phenotypic BACTEC MGIT 960 DST in clinical TB isolates from two countries of the Balkan Peninsula. Methods. Between 1996 and 2017, we retrospectively selected 61 TB isolates. To identify gene variants related to drug resistance in genomic DNA extracted from TB isolates, AmpliSeq libraries were generated automatically using the AmpliSeq™ Kit for Chef DL8 and the Ion AmpliSeq TB Research Panel. Result.s Of all 61 TB isolates included, 56 TB were phenotypically resistant to any antibiotic. Among them, 38/56 (67.9%) TB isolates were phenotypically resistant to pyrazinamide and pncA mutations were detected in 33/38 cases (86.8%). A mutation in the pncA promoter region was the most prevalent genetic alteration, detected in eight TB isolates. Comparison of NGS to conventional BACTEC MGIT 960 DST revealed very strong agreement (90.2%) between the two methods in identifying PZA resistance, with high sensitivity (89.5%) and specificity (95.7%) for NGS. Conclusions. Detection of PZA resistance using NGS seems to be a valuable tool for surveillance of TB drug resistance also in the Balkan Peninsula, with great potential to provide useful information at least one weak earlier than is possible with phenotypic DST. Keywords: tuberculosis, Mycobacterium tuberculosis, high-throughput nucleotide sequencing, pyrazinamide, microbial sensitivity tests, next-generation sequencing, drug susceptibility testing, Slovenia, Republic of North Macedonia Published in DiRROS: 10.01.2022; Views: 907; Downloads: 557 Full text (1,53 MB) This document has many files! More... |
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87. Prehrana in rak : kaj jesti, če zbolimoNada Rotovnik-Kozjek, Denis Mlakar-Mastnak, Katja Kogovšek, Irena Sedej, Eva Peklaj, 2021, not set Keywords: prehranska priporočila, hranilne snovi, enakovredna živila, prehranski dodatki, prehranjevalne motnje, prehranjenost, živila, telesna aktivnost, zdravstveni nasveti Published in DiRROS: 16.11.2021; Views: 924; Downloads: 346 Full text (4,67 MB) |
88. Robust saliva-based RNA extraction-free one-step nucleic acid amplification test for mass SARS-CoV-2 monitoringEva Rajh, Tina Šket, Arne Praznik, Petra Sušjan, Alenka Šmid, Dunja Urbančič, Irena Mlinarič-Raščan, Polona Kogovšek, Tina Demšar, Mojca Milavec, Katarina Prosenc, Žiga Jensterle, Mihaela Zidarn, Viktorija Tomič, Gabriele Turel, Tatjana Lejko-Zupanc, Roman Jerala, Mojca Benčina, 2021, original scientific article Abstract: Early diagnosis with rapid detection of the virus plays a key role in preventing the spread of infection and in treating patients effectively. In order to address the need for a straightforward detection of SARS-CoV-2 infection and assessment of viral spread, we developed rapid, sensitive, extraction-free one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) tests for detecting SARS-CoV-2 in saliva. We analyzed over 700 matched pairs of saliva and nasopharyngeal swab (NSB) specimens from asymptomatic and symptomatic individuals. Saliva, as either an oral cavity swab or passive drool, was collected in an RNA stabilization buffer. The stabilized saliva specimens were heat-treated and directly analyzed without RNA extraction. The diagnostic sensitivity of saliva-based RT-qPCR was at least 95% in individuals with subclinical infection and outperformed RT-LAMP, which had at least 70% sensitivity when compared to NSBs analyzed with a clinical RT-qPCR test. The diagnostic sensitivity for passive drool saliva was higher than that of oral cavity swab specimens (95% and 87%, respectively). A rapid, sensitive one-step extraction-free RT-qPCR test for detecting SARS-CoV-2 in passive drool saliva is operationally simple and can be easily implemented using existing testing sites, thus allowing high-throughput, rapid, and repeated testing of large populations. Furthermore, saliva testing is adequate to detect individuals in an asymptomatic screening program and can help improve voluntary screening compliance for those individuals averse to various forms of nasal collections. Keywords: SARS-CoV-2, COVID-19, COVID-19 serological testing, real-time polymerase chain reaction, saliva, oral cavity swab, passive drool, pooling Published in DiRROS: 09.11.2021; Views: 1087; Downloads: 546 Link to full text |
89. Stereotaktično obsevanje : novi izzivi zdravljenja v radioterapiji : [strokovno srečanje : elektronski zbornik prispevkov : v Ljubljani, 27.11. 2020]2020, professional monograph Abstract: Stereotaktično obsevanje, tehnika obsevanja, s katero dovedemo visoko obsevalno dozo na manjšo tarčo kjerkoli v telesu, predstavlja eno izmed možnosti radikalnega zdravljenja nekaterih tumorjev, zasevkov ali celo benignih stanj. Stereotaktično obsevanje je neinvazivno, stroški zdravljenja so nizki, bolnikom omogoča hitrejšo vrnitev v domače oziroma delovno okolje, ne poslabša kvalitete življenja in ima nizko stopnjo akutne morbiditete in mortalitete. V skrbnih radioterapevtskih postopkih, od izbora primernih bolnikov za stereotaktično obsevanje, priprave na obsevanje in izdelave obsevalnega načrta, kontrole kvalitete obsevanja, izvedbe obsevanja na linearnem pospeševalniku ter bolnikove oskrbe, sodelujemo zdravniki onkologi radioterapevti, radiologi, medicinski fiziki, dozimetristi, radiološki inženirji, medicinske sestre in zdravstveni tehniki. Skupen cilj naše timske obravnave in naporov medicinskega osebja je predvsem izboljšanje rezultatov zdravljenja ter ohranitev oziroma izboljšanje kvalitete življenja vsakega posameznega bolnika. Veliko pozornosti posvečamu tudi zmanjševanju tveganja za pojav kratkoročnih ali dolgoročnih neželenih učinkov stereotaktičnega obsevanja. Keywords: radioterapija, elektronske knjige Published in DiRROS: 08.12.2020; Views: 2026; Downloads: 520 Full text (14,11 MB) |
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