1. Programmable protein-DNA composite nanostructures : from nanostructure construction to protein-induced micro-scale material self-assembly and functionalizationWeijun Zhou, Žiga Strmšek, Jaka Snoj, Miha Škarabot, Roman Jerala, 2025, original scientific article Abstract: The integration of DNA and protein-designed nanostructures represents a transformative approach to the development of programmable biopolymers for nanoscale construction. While DNA nanostructures excel in the readily programmable precision and scalability of base pairing, protein assemblies exploit the chemical diversity of amino acids for greater functional versatility. Here a platform is presented that unifies these two paradigms by combining coiled-coil protein origami with DNA nanostructures through orthogonal protein-protein (SpyCatcher-SpyTag) and protein-DNA (DCV-DNA) covalent conjugation strategies. This dual-functionalization strategy enables the construction of stable and versatile protein-DNA composites capable of hierarchical self-assembly. This shows that these composites drive the transformation of DNA nanotubes into large-scale, patterned nanofibers or nanorods, with the proteins regularly distributed over their surface and retaining their enzymatic and fluorescent functions. In addition, a DNA-luciferase circuit is developed through split enzyme reconstitution to achieve reversible regulation of enzymatic activity, highlighting the dynamic functionality of these composites. This introduces a modular approach to producing multifunctional bio-nanomaterials, highlighting the potential of protein-DNA composite nanostructures as a bridge between molecular design and functional nanomaterials and paves the way for the development of dynamic bio-devices and programmable biomaterials. Published in DiRROS: 02.07.2025; Views: 115; Downloads: 55
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2. Beyond dimerization : harnessing tetrameric coiled-coils for nanostructure assemblySara Vidmar, Tamara Šmidlehner, Jana Aupič, Žiga Strmšek, Ajasja Ljubetič, Fei Xiao, Guang Hu, Chuan Liu, Florian Beck, Philipp S. Erdmann, Roman Jerala, 2025, original scientific article Published in DiRROS: 31.03.2025; Views: 365; Downloads: 168
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3. Selective inhibition of NLRP3 inflammasome by designed peptide originating from ASCPetra Sušjan, Duško Lainšček, Žiga Strmšek, Vesna Hodnik, Gregor Anderluh, Iva Hafner Bratkovič, 2020, original scientific article Keywords: blood-brain barrier, inflammation, ▫$IL-1beta$▫, peptide inhibitors Published in DiRROS: 24.03.2022; Views: 1613; Downloads: 896
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5. Design of coiled-coil protein-origami cages that self-assemble in vitro and in vivoAjasja Ljubetič, Fabio Lapenta, Helena Gradišar, Igor Drobnak, Jana Aupič, Žiga Strmšek, Duško Lainšček, Iva Hafner Bratkovič, Andreja Majerle, Nuša Krivec, Mojca Benčina, Tomaž Pisanski, Tanja Ćirković-Veličković, Adam Round, José María Carazo, Roberto Melero, Roman Jerala, 2017, original scientific article Published in DiRROS: 17.04.2018; Views: 5217; Downloads: 3250
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