1011. Effect of double-step and strain-assisted tempering on properties of medium-carbon steelPavel Salvetr, Andrea Školáková, Jakub Kotous, J. Drahokoupil, Daniel Melzer, Zdeňek Jansa, Črtomir Donik, Aleksandr Gokhman, Zbyšek Nový, 2023, original scientific article Keywords: medium-carbon steel, tempering, strengthening, mechanical properties, microstructure
Published in DiRROS: 02.02.2024; Views: 246; Downloads: 106
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1012. Effect of Cu alloying on mechanical properties of medium-c steel after long-time tempering at 500 °CPavel Salvetr, Aleksandr Gokhman, Milan Svoboda, Črtomir Donik, Ivana Podstranská, Jakub Kotous, Zbyšek Nový, 2023, original scientific article Keywords: long-time tempering, strengthening, Cu precipitate, medium-C steel, mechanical properties
Published in DiRROS: 02.02.2024; Views: 300; Downloads: 107
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1014. The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.)Zina Devetak, Andreja Kavčič, Maarten De Groot, Barbara Piškur, 2023, original scientific article Abstract: One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process.
Keywords: early detection, specimen storage, total DNA extraction, PCR, polymerase chain reaction, Sanger sequencing, molecular diagnostics
Published in DiRROS: 02.02.2024; Views: 647; Downloads: 212
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1015. Dispersoids in Al-Mg-Si alloy AA 6086 modified by Sc and YFranc Zupanič, Sandi Žist, Mihaela Albu, Ilse Letofsky-Papst, Jaka Burja, Maja Vončina, Tonica Bončina, 2023, original scientific article Keywords: aluminium alloy, heat treatment, microstructure, dispersoids, dilatometry, DSC, hardness
Published in DiRROS: 02.02.2024; Views: 309; Downloads: 118
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1016. Complex interdependency of microstructure, mechanical properties, fatigue resistance, and residual stress of austenitic stainless steels AISI 304LPatricia Jovičević Klug, Matic Klug Jovičević, Michael Rohwerder, Matjaž Godec, Bojan Podgornik, 2023, original scientific article Keywords: stainless steel, AISI 304L, deep cryogenic treatment (DCT), microstructure, mechanical properties, fatigue, residual stress
Published in DiRROS: 02.02.2024; Views: 287; Downloads: 116
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1017. Inflammatory, oxidative stress and small cellular particle response in HUVEC induced by debris from endoprosthesis processingZala Jan, Matej Hočevar, Veno Kononenko, Sara Michelini, Neža Repar, Maja Caf, Boštjan Kocjančič, Drago Dolinar, Slavko Kralj, Darko Makovec, Aleš Iglič, Damjana Drobne, Monika Jenko, Veronika Kralj-Iglič, 2023, original scientific article Keywords: endoprosthesis failure, corundum, cytokines, reactive oxygen species, lipid droplets
Published in DiRROS: 02.02.2024; Views: 296; Downloads: 113
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1019. Mechanical properties of high carbon low-density steelsJiri Hajek, Zbyšek Nový, Ludmila Kucerova, Hana Jirková, Črtomir Donik, Zdeňek Jansa, 2023, original scientific article Keywords: low density steels, heat treatment, tensile test
Published in DiRROS: 02.02.2024; Views: 260; Downloads: 106
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1020. Solidification behavior of Fe-6.5Si alloy powder for AM-SLM processing, as assessed by differential scanning calorimetryDarja Steiner Petrovič, Črtomir Donik, Irena Paulin, Matjaž Godec, Maja Vončina, Martin Petrun, 2023, original scientific article Keywords: soft magnetic alloy, differential scanning calorimetry, oxygen, high-temperature phase, microstructure, eutectics
Published in DiRROS: 02.02.2024; Views: 244; Downloads: 103
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