1181. Reorganization of microtubules in V-79 cells after treatment with cytohalasin BAleš Iglič, Urška Batista, Peter Veranič, 1999, original scientific article Abstract: Background. The aim of this work was to study the configuration of the microtubules in the cytochalasin B treated V-79 cells in connection to the cell shape and to see whether there are any similarities to the phenomena taking place in phospholipid vesicles. Subjects and methods. An experiment wasperformed where cytochalasin B was added to the V-79 cells (lung fibroblasts of Chinese hamster). Results. The cell shape changed from an elongated one into the shape with a profile resembling the Greek letter phi. The microtubules were found to be organized into a rod within the symmetry axis of the cell. Conclusion. As similar shapes were previously observed also in the phospholipid vesicles with entrapped microtubule rods, our results support the hypothesis that similar physical mechanisms may pertain in simple systems as well as in living cells.
Published in DiRROS: 22.01.2024; Views: 286; Downloads: 49
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1183. Indium-111-DTPA-octreotide scintigraphy in patients with carcinoid tumorStanko Težak, Rajko Ostojić, Zdravko Perković, Nadan Rustemović, Nikica Car, Branko Papa, Mirjana Poropat, Damir Dodig, 1999, original scientific article Abstract: Background. The aim of the study was the evaluation of clinical utility and comparison of 111 In-DTPA- octreotide receptor scintigraphy (SRS) with conventional imaging modalities (CIM) in the detection of carcinoid tumor. Patients and methods. Fourteen patients with pathohistologically proven diagnosis of carcinoid tumor and one patient with clinical suspicion of carcinoid tumor were investigated by SRS. SRS was performed for localization of primary tumor, recurrence or estimation of spread of the disease after CIM had been completed. Whole body scans and single photon emission computed tomography (SPECT) were acquired 6 and 24 h after the application of radiopharmaceutical. The intensity of nonspecific radiopharmaceutical uptake in the bowel was assessed semiquantitatively by a score using whole body scans. Results. The evaluation was done for patients and for tumor sites. The sensitivity, specificity, and positive and negative predictive values for patient evaluation were 89 %,100 %,100 % and 80 %, respectively for both CIM and SRS, whereas for tumor sites, these parameters were 69 % 100 %,100 % and 82 % for CIM, and 88%,100%,100% and 92 % for SRS. Intensity score of nonspecific 111In-octreotide bowel accumulation was 0.92 and 2.01 for 6 and 24h scans respectively (p < 0.01). Conclusion. 111In-octreotide scintigraphy should be included in the diagnostic algorithm for the patients with clinical suspicion of carcinoid and for the assessment of patients with proven carcinoid tumor.
Published in DiRROS: 22.01.2024; Views: 222; Downloads: 45
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1184. Comparison of colorimetric MTT and clonogenic assays for irradiation and cisplatin treatment on murine fibrosarcoma SA-1 cellsMaja Čemažar, Darja Marolt, Mira Lavrič, Gregor Serša, 1999, original scientific article Abstract: Background. The aim of our study was to determine the relationship between cell survival of SA-1 tumor cells measured by clonogenic assay and MTT assay after irradiation and cisplatin treatment. Materials and methods. Survival of SA-1 cells was measured after irradiation (2-8 Gy) and cisplatin treatment (0.05-0.5 u g/ml) by clonogenic assay performed 7 days after treatment, and byMTT assay performed on day 3, 4, 5, and 7 after the treatment. Results. The results showed good correlation between MTT assay and clonogenic assay for irradiation doses below 4 Gy. For higher doses good correlation between MTT and clonogenic assay was determined only when MTT assay was performed on day 5and 7 after the treatment. In the case of cisplatin treatment, similar pattern was observed, good correlation between IC50 values for MTT and clonogenic assay was found when MTT assay was performed on day 5 and 7 after the treatment. Conclusion. Results of our study confirmed the results of previous studies addressing this topic and further support the use of MTT testas an alternative test for clonogenic test as a predictive assay of tumourresponse to the radio or chemotherapy.
Published in DiRROS: 22.01.2024; Views: 278; Downloads: 57
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1185. Adenocarcinoma skin metastases treated by electrochemotherapy with cisplatin combined with radiationGregor Serša, Maja Čemažar, Zvonimir Rudolf, Albert-Peter Fras, 1999, original scientific article Abstract: Background. The aim of this study was to determine the interaction between electrochemotherapy as a means of facilitated cisplatin delivery into the cells and irradiation of adenocarcinoma skin metastases. Case report. A patient with progressive disease presenting skin metastases of tubal dedifferentiated pappilary adenocarcinoma was enrolled in the study. Skin metastases were treated by electrochemotherapy with intratumoral injection of cisplatin. Its antitumor effectiveness was compared to that of combined treatment of irradiation with electrochemotherapy. After a two week observation time, the response to treatment was comparable between the electrochemotherapy and electrochemotherapy combined with irradiation. Both ofthese treatments were more effective than irradiation alone. Furthermore, antitumor effectiveness of the combined electrochemo- and radiotherapy was found to be quicker than that of electrochemotherapy alone. Comment. This study shows that electrochemotherapy with cisplatin is also effective in the treatment of adenocarcinoma skin metastases. Inspite of the short observation time, positive interaction between radiotherapy and electrochemotherapy with cisplatin was found.
Published in DiRROS: 22.01.2024; Views: 231; Downloads: 63
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1186. Acute subarachnoid haemorrhage : detection of aneurysms of intracranial arteries by computed tomographic angiographyZoran Miloševič, 1999, original scientific article Abstract: Background. We wanted to determine the diagnostic accuracy, sensitivity and specificity of computed tomographic angiography (CTA) of intracranial vessels,and to establish the advantages and disadvantages of CTA compared to digital subtraction angiography (DSA) as the gold standard in patients with acute subarachnoid haemorrhage (SAH). Patients and methods. We prospectively studied 52 patients with acute SAH. Confirmation of the haemorrhage by a conventional computed tomography (CT) scan was immediately followed by intracranial CTA. DSA was performed after the CTA examination and so did not influence the interpretation of CTA images. The sensitivity, specificity and diagnostic accuracy of CTA were determined by comparing the results with the data from DSA and with the surgical findings. Cases where the CTA and DSA results did not match were analysed, and the advantages and disadvantages of intracranial CTA were determined. Results. The diagnostic accuracy of CTA was 95%, its sensitivity was 93%, and its specificity was 98%. False-negative results were obtained in three patients who harboured small aneurysms, two in the region of the cavernous sinus and one at the division of pericallosal and callosomarginal arteries. In one patient with a false-positive result, DSA showed an infundibular widening of the posterior communicating artery. In all seven patients who underwent operations on the basis of CTA results, the surgical findings confirmed the presence of aneurysms as well as the intracranial vessel anatomy demonstrated by CTA. Conclusions. Intracranial CTAis a fast and minimally invasive method with a high diagnostic accuracy, sensitivity and specificity, which has an important place in the detection andpreoperative evaluation of intracranial aneurysms in patients with acute SAH.
Published in DiRROS: 22.01.2024; Views: 207; Downloads: 50
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1187. Ultrasound in diagnosis and treatment of anal fistulasPavle Košorok, 1999, original scientific article Abstract: Buckground. Endoanal ultrasound (EUS) is helpful in the diagnosis of anorectalfistulas and abscesses due to its high resolution image quality and anatomical orientation using a radial scanning echoprobe. This type of information is essential in monitoring the response to treatment. Initial EUS examination prior to treatment, is mandatory in order to compare the results before and after treatment. Intraoperative EUS may also be helpful if the lesion is deep beyond the pelvic floor muscle which may not be seen during surgery. Better results in EUS imaging of perianorectal fistulas can be obtained using hydrogen peroxide as a contrast medium. Concomitant advantage of hydrogen peroxide ultrasound was the detection of suspected or unsuspected sphincter defects. However, interobserver reliability has to be taken into account together with the learning curve of a particular observer. Conclusions. Endoanal US is a safe, economic, and reliable procedure for the assessment of fistula-in-ano, and is superior to physical examination.
Published in DiRROS: 22.01.2024; Views: 222; Downloads: 50
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1190. How reliable is classic chest radiography in the diagnosis of small pleural effusionIgor Kocijančič, Ksenija Kocijančič, 1999, original scientific article Abstract: Purpose. To evaluate the usefulness of expirium lateral decubitus views in theradiological diagnosis of small pleural effusions. Materials and methods. Patients referred to abdominal sonography for different reasons were routinelychecked for possible pleural effusion. From November 1994 till May 1996, 36 such patients were found to have pleural effusion not exceeding 15 mmand were included in the study. Patients were examined radiologically in erect PA and lateral projections and, after 5 min. in decubitus position, in inspiratory-expiratory lateral decubitus projections with 10 hip elevation andcentral beam on the lateral chest wall. Results. In 22 out of 36 patients (61 %), the pleural fluid was not visible on erect PA and lateral chest radiogram. However, the fluid was visible in 35/36 patients (97%) in expirium from lateral decubitus view. The average thickness of fluid from lateral decubitus views in inspirium and expirium was 4.3 and 7.9 mm, respectively. In31 out of 36 patients (86%), the thickness of the fluid layer as measured inexpirium and inspirium was different. In 16%, the fluid was not visible on inspirium lateral decubitus projections. Conclusions. Radiography turned out to be almost as sensitive as sonography in detection of small pleural effusions. Lateral decubitus views taken in expirium contributed essentially to the diagnostic sensitivity in our study.
Published in DiRROS: 22.01.2024; Views: 232; Downloads: 57
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