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91.
Letno poročilo za leto 2014
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Published in DiRROS: 17.12.2024; Views: 31; Downloads: 14
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92.
Letno poročilo za leto 2015
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Published in DiRROS: 17.12.2024; Views: 32; Downloads: 15
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93.
Letno poročilo za leto 2017
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Published in DiRROS: 17.12.2024; Views: 32; Downloads: 14
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94.
Letno poročilo NIB za leto 2018
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Published in DiRROS: 17.12.2024; Views: 32; Downloads: 16
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95.
Letno poročilo za leto 2019
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Published in DiRROS: 17.12.2024; Views: 33; Downloads: 14
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96.
Letno poročilo za leto 2020
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Published in DiRROS: 17.12.2024; Views: 36; Downloads: 16
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97.
Letno poročilo za leto 2021
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Published in DiRROS: 17.12.2024; Views: 35; Downloads: 15
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98.
Letno poročilo za leto 2022
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Published in DiRROS: 17.12.2024; Views: 31; Downloads: 14
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99.
A new quantitative PCR assay for detection of potentially anatoxin-producing cyanobacteria
Maša Jablonska, Tina Eleršek, 2024, original scientific article

Abstract: Anatoxins (ATX) are globally occurring toxins produced by some species of cyanobacteria in aquatic habitats. They can cause acute poisoning in animals, leading to muscle paralysis and respiratory failure, and might also pose a long-term health risk to humans. Thanks to advances in molecular methods and genomic knowledge, it is now possible to rapidly detect and quantify the genes associated with cyanotoxin production for most major groups of cyanotoxins except ATX. The aim of this study was to develop and validate a new quantitative PCR (qPCR) assay for general detection of all potential ATX producers in the environment. After specificity testing in silico and in vitro with 16 cyanobacterial strains (endpoint PCR, amplicon sequencing and qPCR), two assays targeting the anaC gene were thoroughly validated for linearity, amplification efficiency, sensitivity, dynamic range, inter-assay and intra-assay variability, and the influence of background DNA. The assays were then applied to 144 environmental samples of plankton and biofilm from lakes and rivers whose ATX content had previously been measured by liquid chromatography with tandem mass spectrometry (LC-MS/MS). Amplification efficiency of the two designed assays was between 94% and 103%, and the limits of quantification and detection were up to, but mostly below, 322 and 32 cells/mL, respectively. Both assays showed better or equal specificity in cyanobacterial cultures than currently available PCR assays and were able to predict the presence of ATX detected by LC-MS/MS in most environmental samples (83 % in plankton and 52–62% in biofilm). A higher number of discrepancies between qPCR and LC-MS/MS results in biofilm than in plankton samples indicates limited knowledge and sparse genomic data on benthic cyanobacteria. These assays are the first published general qPCR assays targeting all ATX producers and could provide water managers with a rapid and cost-effective risk assessment to better protect human and animal health.
Keywords: anatoxins (ATX), cyanobacteria, qPCR assay, anaC gene, LC-MS/MS, environmental microbiology, environmental science, toxicology, molecular biology
Published in DiRROS: 17.12.2024; Views: 40; Downloads: 16
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100.
Assessing the heat storage potential of zeolitic imidazolate frameworks (ZIFs) using water and ethanol as working fluids
Ciara Susan Byrne, Matjaž Mazaj, Nataša Zabukovec Logar, 2025, original scientific article

Published in DiRROS: 17.12.2024; Views: 39; Downloads: 25
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