1201. Assessment of renal function from creatinine clearance measurement and 131I-hippuran renography in cancer patients before chemotherapyBorut Štabuc, Tine Hajdinjak, Tomaž Edvard Cizej, 1999, original scientific article Abstract: Background. Serum creatinine and endogenous creatinine clearance (CrCl) are widely used measures of renal function before prescribing nephrotoxic chemotherapy. This study compares the precision and bias in glomerular filtration rate (GFR) estimation without the need to collect urine by using Cockcroft-Gault formula on a single serum creatinine concentration (CrCo) and 131I- hippuran clearance (HC) determined from the renographic curves. Patientsand methods. Fourty-seven patients aged between 27 and 73 years were studied. In all patients, we determined serum creatinine concentration, CrCl, CrCo and HC simultaneously before treatment by combined chemotherapy with cisplatin (CDDP) and in 31 patients, before the third cycle. Serum and urine creatinine concentrations were determined with a Hitachi 911, an automated biochemical analyser CrCl was calculated from the urine flow, from the ratio between the serum and urine creatinine concentrations and was standardized forthe body surface area. Serum creatinine was used to estimate CrCo using a Cockcroft and Gault formula. HC was determined from 131I-hippuran uptake by both kidneys, results were compared to our Nuclear Medicine Department normal values with regard to the age of each patient. For the evaluation of results, Pearson's correlation coefficient and t-test with 95 % confidence interval were used. Results. The sensitivity of serum creatinine, CrCo and HC to predict CrCl<78 mL/min/1.73m2 was 41 %, 68% and 46% and specificity was 95%, 71 % and 76% respectively. Value of CoCr for prediction of reduced CrCl (sensitivity) was statistically significantly better than the HC (p=0.03). Value of CoCr fnr prediction of normal CrCl (specificity) was as good as HC (p=0.3). Conclusions. CrCl for the GFR estimation in the patients treated withnephrotoxic chemotherapy cannot be changed by CrCo and/or HC.
Published in DiRROS: 22.01.2024; Views: 272; Downloads: 70
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1202. Ultrasound diagnosis of gallstone ileus - a case reportDubravka Vidmar, Stanislav Repše, 1999, professional article Abstract: Background. The diagnostic method of choice for ileus was a plain abdominal film. However, in the recent years, x-ray has been increasingly replaced by ultrasonography which, in experienced hands, can reveal ileus at an earlier stage and also identify the cause and site of obstruction. Case report. We reported about a male patient who was admitted to the Department of Gastroenterologic Surgery with severe abdominal pain and vomiting. A plain abdominal film showed an obstructive ileus and pneumobilia. Ultrasound examination of the abdomen confirmed the presence of ileus of the small intestine and demonstrated a gallstone impacted in the distal ileum. It also disclosed a distorted gallbladder adhering to the duodenum. The patient was treated surgically and had an uneventful postoperative course. Conclusions. Ultrasonography, in addition to diagnosing the ileus caused by an ectopic gallstone, is also able to identify the aetiology and site of obstruction.
Published in DiRROS: 22.01.2024; Views: 305; Downloads: 63
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1203. Epithermal neutron beam for BNCT at the JSI TRIGA reactor - modelling and experimental verificationMarko Maučec, Bogdan Glumac, Jože Rant, Edvard Krištof, 1999, original scientific article Abstract: It has been reported that satisfactory thermal/epithermal neutron beams for Boron lVeutron Ćapturc, Therapy (BNCT) could be designed at TRIGA research reactors, which are generally perceived as being safe to install and operate in populated areas. This contribution presents the most recent research activities in this field at the Jožef Stefan Institute TRIGA reactor, where anepithermal neutron beam for BNCT is being developed. Experimental verification of Monte Carlo simulation results proves the quality and wide applicability of the developed 3-D model, particularly of the reactor core andirradiation channels. Due to high attenuation of the epithermal neutron flux (fi epi = 4.1x106 n/cmZs, two orders of magnitude belozu the therapeutic limit) the irradiation facility in the current stage of development is not appropriate for the clinical BNCT treatments. Furthermore, the contribution ofthe 2.5 mm airgap surrounding the facility is unacceptably high, thus makingthe relative gamma dose (Dy/fiepi) almost 60-times higher than therapeutically rcc- ommended. Nevertheless, using gamma shielding of Pb or Biand LiF or Li2C03 (thermal neutron cut-off, the quality of the epithermal neutron beam would be significantly upgraded and hecome appropriate fnr iii vitro studies of boron compound transport in malignant tumour cells or smallerlahoratory animals.
Published in DiRROS: 22.01.2024; Views: 192; Downloads: 43
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1205. Description, identification, and growth of Tuber borchii Vittad. mycorrhized Pinus sylvestris L. seedlings on different lime contentsTanja Mrak, Tine Grebenc, Silke Friedrich, Babette Münzenberger, 2024, original scientific article Abstract: Tuber borchii forms ectomycorrhiza with oaks, hazel, and pines, including Pinus sylvestris. However, its ectomycorrhiza morphotype with P. sylvestris was not comprehensively described so far, and molecular analyses are missing despite a high danger of misidentification of T. borchii ectomycorrhiza with other closely related and less valuable truffle species. We described for the first time the morphology and anatomy of T. borchii-P. sylvestris ectomycorrhiza using differential interference contrast technique and semi-thin sections in combination with molecular confirmation of identity. Color of ectomycorrhiza is reddish to dark brown, and morphotypes are unevenly but densely covered by warts-bearing pin-like cystidia. All layers of the hyphal mantle are pseudoparenchymatous with outer mantle layer formed of epidermoid cells. T. borchii ectomycorrhiza was identified by a molecular comparison with fruitbodies used for inoculation and its respective ectomycorrhizae. T. borchii has a wide ecological amplitude. To get a better insight in mycorrhization requirements, we investigated growth of P. sylvestris and its ectomycorrhiza infection rate with T. borchii in substrate with different lime content. The mycorrhization of P. sylvestris with T. borchii in the mycorrhization substrate and cultivation in greenhouse conditions was successful, with colonization of P. sylvestris varying between 36.5 and 48.1%. There was no significant correlation of mycorrhization to applied lime contents, and consequently to pH in substrate, while the increased levels of lime improved growth of the P. sylvestris seedlings.
Keywords: root anatomy, ectomycorrhiza, Limestone content, molecular identification, Pinus sylvestris, Tuber borchii
Published in DiRROS: 22.01.2024; Views: 522; Downloads: 322
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1206. Monitoring of species’ genetic diversity in Europe varies greatly and overlooks potential climate change impactsPeter Pearman, Olivier Broennimann, Tsipe Aavik, Tamer Albayrak, Paulo Célio Alves, Filipos Aravanopoulos, Laura Bertola, Aleksandra Biedrzycka, Elena Bužan, Vlatka Čubrić Čurik, Katja Kavčič Sonnenschein, Marjana Westergren, 2024, original scientific article Abstract: Genetic monitoring of populations currently attracts interest in the context of the Convention on Biological Diversity but needs long-term planning and investments. However, genetic diversity has been largely neglected in biodiversity monitoring, and when addressed, it is treated separately, detached from other conservation issues, such as habitat alteration due to climate change. We report an accounting of efforts to monitor population genetic diversity in Europe (genetic monitoring effort, GME), the evaluation of which can help guide future capacity building and collaboration towards areas most in need of expanded monitoring. Overlaying GME with areas where the ranges of selected species of conservation interest approach current and future climate niche limits helps identify whether GME coincides with anticipated climate change effects on biodiversity. Our analysis suggests that country area, financial resources and conservation policy influence GME, high values of which only partially match species’ joint patterns of limits to suitable climatic conditions. Populations at trailing climatic niche margins probably hold genetic diversity that is important for adaptation to changing climate. Our results illuminate the need in Europe for expanded investment in genetic monitoring across climate gradients occupied by focal species, a need arguably greatest in southeastern European countries. This need could be met in part by expanding the European Union’s Birds and Habitats Directives to fully address the conservation and monitoring of genetic diversity.
Keywords: genetics, monitoring, population genetic diversity, Europe
Published in DiRROS: 22.01.2024; Views: 352; Downloads: 225
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1207. Prognostic relevance of urokinase plasminogen activator and its inhibitors in patients with breast cancerSimona Borštnar, Tanja Čufer, Ivan Vrhovec, Zvonimir Rudolf, 1999, original scientific article Abstract: Urokinase plasminogen activator (uPA) and its inhibitors, PAI-1 and PAI-2, play an important role in iritercellular tissue degradation, thus promoting tumor cell invasion into the adjoining structures and metastasizing. Our studywas aimed to assess a possible prognostic value of uPA, PAI-1 and PAI-2 in a retrospective series of 87 patients with breast cancer stage I-III, whosecytosols were stored in the archives of the, Institute of Oncology in Ljubljana. The median follow-up was 35 months. The prognostic value of the established prognostic factors and uPA, PAI-1 and PAI-2 were evaluated by means of univariate statistical analysis and partial multivariate models. The obtained uPA values were very low and did not correlate with the disease free survival, whereas PAI-1 and PAI-2 significantly influenced the time to the first recurrence. Patients with PAI-1 values above 5 ng/mg proteins had statistically significantly worse disease free survival than the patients withlower PAI-1 values (58% vs. 85%). In the case of PAI-2, the situation wasjust the opposite: the patients with PAI-2 values exceeding 6.4 ng/mg proteins had statistically significantly better 3-year disease free survival than the patients with lower values (90% vs. 60%). Both, PAI-1 and PAl-2 retained their independent prognostic value, irrespective of the addition of the established prognostic factors to partial multivariate models, and only with locally advanced disease the prognostic value of PAI-1 was greater than that of PAI-2.
Published in DiRROS: 22.01.2024; Views: 289; Downloads: 78
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1208. A correlation of NK cytolitic test and BLT esterase test in determining activity of NK cells, stimulated by tumor target cellsAlojz Ihan, 1999, original scientific article Abstract: We examined the granule exocytosis in natural killer (NK) cells by measuring N-benzyloxycarboxy-L-lysine esterase activity. As a source of NK cells we usedbuffy-coat isolated NK cells or peripheral blood lymphocytes (PBL). The exocytosis was triggered by incubating cells with ionomycin/PMA or by NK cell susceptible tumour target cells K562. When we stimulated purified NK cells with tumour target cells, a close correlation (Corr=0.84) of cytolitic NK testresults and BLT test results was obtained. We may conclude that BLT test can provide a valuable tool to discriminate further NK cell deficiency in patients with low cytolitic NK test results.
Published in DiRROS: 22.01.2024; Views: 225; Downloads: 85
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1209. Predictive assays of tumor response to chemo and radiotherapyMaja Čemažar, 1999, review article Abstract: Predstavljeni so testi za napovedovanje izida zdravljenja tumorjev po kemo- inradioterapiji in poškodb normalnih tkiv po radioterapiji. Pregled je osredotočen na tiste teste, pri katerih je treba celice gojiti v pogojih in vitro. Poleg tega članek obravnava tudi korelacijo med napovednimi testi in izidom zdravljenja različnih vrst raka ter poškodbami normalnih tkiv.
Published in DiRROS: 22.01.2024; Views: 254; Downloads: 70
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1210. Use of steel slag for the synthesis of belite-sulfoaluminate clinkerLea Žibret, Martina Cvetković, Maruša Mrak, Mojca Loncnar, Andrej Ipavec, Sabina Dolenec, 2020, published scientific conference contribution Abstract: Belite-sulfoaluminate (BCSA) cements are low-carbon mineral binders, which require low energy consumption and allow the incorporation of various secondary raw materials in the clinker raw meal. In this study two types of unprocessed steel slags, coming from stainless steel production, were incorporated in the BCSA clinkers. The clinker phase composition, clinker reactivity, and the compressive strength of the cement were studied to evaluate the possible use of the slag in BCSA clinkers. The cement clinkers were synthesized by using natural raw materials, white titanogypsum, mill scale, as well as two different steel slags: (i) EAF S slag, which is a by-product of melting the recycled steel scrap in an electric arc furnace, and (ii) la dle slag as a by-product of the processes of secondary metallurgy, in various quantities. Raw mixtures with two different targeted phase compositions varying in belite, calcium sulfoaluminate and ferrite phases were sintered at 1250 °C. Clinker phases were determined by Rietveld quantitative phase analysis, while their distribution, morphology and incorporation of foreign ions in the phases were studied by SEM/EDS analysis. The clinker reactivity was determined by isothermal calorimetry. BCSA cements were prepared by adding titanogypsum. The compressive strength of the cement pastes was determined after 7 days of hydration. The presence of a predicted major clinker phases was confirmed by Rietveld analysis, however periclase was also detected. Microscopy revealed subhedral grains of belite and euhedral grains of calcium sulfoaluminate phases, while ferrite occurred as an interstitial phase. The results showed differences in the microstructure and reactivity of the clinker and cement, which can be attributed to varying amounts of ettringite due to different slag types
Keywords: belite-sulfoaluminate cement, cement clinker, steel slags, clinker microstructure, cement reactivity
Published in DiRROS: 19.01.2024; Views: 360; Downloads: 138
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