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Determination of the activity signature of key carbohydrate metabolism enzymes in phenolic-rich grapevine tissuesElizabeth Dunn Covington,
Thomas Roitsch,
Marina Dermastia, 2016, original scientific article
Abstract: Physiological studies in plants often require enzyme extraction from tissues containing high concentrations of phenolsand polyphenols. Unless removed or neutralized, such compounds may hinder extraction, inactivate enzymes, and inter-fere with enzyme detection. The following protocol for activity assays for enzymes of primary carbohydrate metabo-lism, while based on our recently published one for quantitative measurement of activities using coupled spectrophoto-metric assays in a 96-well format, is tailored to the complexities of phenolic- and anthocyanin-rich extracts from grape-vine leaf. As a case study we applied the protocol to grapevine leaf samples infected with plant pathogenic bacteriašCandidatusPhytoplasma solani’, known to alter carbohydrate metabolism in grapevine. The described adaptations maybe useful for determination of metabolic fingerprints for physiological phenotyping of other plant species with inhe-rently high levels of phenolic compounds.
Keywords: AGPase, carbohydrates, invertases, sucrose synthase, panel of enzyme activity assays, phytoplasma
Published in DiRROS: 25.07.2024; Views: 129; Downloads: 75
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