1951. Insights into the osteosarcoma microenvironment : multiscale analysis of structural and mineral heterogeneityFrancesca Rossi, Rydzyk Martyna Malgorzata, Luisa Barba, Emil Malucelli, Maria Elisabetta Federica Palamà, Chiara Gentili, Maddalena Mastrogiacomo, Alessia Cedola, Lucia Mancini, Murielle Salomè, Hiram Castillo Michel, Davide Maria Donati, Marco Gambarotti, Enrico Lucarelli, Michela Fratini, Stefano Iotti, 2025, original scientific article Abstract: Osteosarcoma (OS) is a malignant and heterogeneous disease that typically originates in the long bones of children and adolescents. It is characterized by the presence of immature cells having an aggressive phenotype and rapid uncontrolled proliferation. OS progression induces significant molecular and cellular changes locally within the bone, resulting in the development of an abnormal tumor microenvironment (TME). The OS TME plays a crucial role in tumor progression and development, however, the precise effects of OS on bone structure and mineralization still remain poorly understood. In this study, we examined the OS TME by analyzing samples from osteoblastic, parosteal, and periosteal osteosarcomas. Employing advanced synchrotron-based X-ray techniques, we performed a multiscale analysis to evaluate the structural and mineral complexity of tumor-affected bone. Our results revealed marked morphological differences among the osteosarcoma subtypes, while con- firming that biomineralization remains active through the production of hydroxyapatite (HA). X-ray diffraction identified two distinct hydroxyapatite crystalline phases across all samples, suggesting a critical behavior of minerals in bone. Additionally, we observed that the bone mineral structure in periosteal and parosteal osteosarcomas exhibited crystal deformations along the c-axis, whereas the osteoblastic osteosarcoma displayed a mineral profile comparable to control bone. Micro-X-ray absorption near-edge spectroscopy revealed the occurrence of a dysregulated biomineralization in the parosteal and periosteal subtypes, marked by the presence of calcium compounds different from HA, in contrast to the mature mineral state found in the osteoblastic variant. These findings highlight the complexity of osteosarcoma repercussion on bone tissue, offering new in- sights into the interactions within the OS TME.
Keywords: osteosarcoma, biomineralization, XANES, synchrotron-based X-ray diffraction, X-ray computed microtomography
Published in DiRROS: 10.06.2025; Views: 584; Downloads: 441
 Full text (7,09 MB)
This document has many files! More... |
1952. Acta chimica slovenica2000, not set Abstract: Acta Chimica Slovenica (ACSi) is an international, peer-reviewed and Open Access journal. It provides a forum for the publication of original scientific research in all fields of chemistry and closely related areas. Reviews, feature, scientific and technical articles, and short communications are welcome.
In the appendix, the journal publishes book reviews, industrial achievements, announcements, and Society news.
Articles in ACSi are published under the Creative Commons Attribution 4.0 International License.
Keywords: kemija, znanstveni članki, raziskave
Published in DiRROS: 10.06.2025; Views: 641; Downloads: 179
 Link to file
This document is also a collection of 4 documents! |
1953. |
1954. Europa come modo di abitare : Conferenza internazionale2025, not set Keywords: filozofija, fenomenologija, Evropa, prebivanje, družbenost, skupnost, interdisciplinarnost, mednarodna posvetovanja, zborniki
Published in DiRROS: 10.06.2025; Views: 513; Downloads: 165
Full text (1,99 MB) |
1955. Student and parental perception on homework : evidence from PIRLS for SloveniaEva Klemenčič Mirazchiyski, Plamen Mirazchiyski, 2025, original scientific article Keywords: vzgoja in izobraževanje, domače naloge, učenci, starši, bralna pismenost, PIRLS 2021, Slovenija, education, homework, students, parents, reading literacy, PIRLS 2021, Slovenia
Published in DiRROS: 10.06.2025; Views: 547; Downloads: 304
Full text (322,63 KB)
This document has many files! More... |
1956. |
1957. |
1958. |
1959. |
1960. |
