341. Ženska misel in čustvovanje nekdaj in danes : literarnopragmatični vidikMaša Rolih, Diana Košir, 2025, independent scientific component part or a chapter in a monograph Abstract: V prispevku so v ospredju literarne protagonistke v zbirki črtic Zofke Kveder Misterij žene (1900) ter ženski glas v pesniških zbirkah Drseči svet (2020) in Rodna doba (2024) sodobne pesnice Nine Medved. Tematska presečišča so izzivi ženskega subjekta (npr. samoiskanje, partnerstvo in intima, materinstvo, (ne)želena nosečnost). V izbranem gradivu so bile analizirane jezikovnoslogovne prvine, ki v (literarnem) diskurzu nastopajo v vlogi označevalcev intenzitete, z namenom komparativne analize ženske misli v diahroni perspektivi.
Keywords: žensko vprašanje, intenziteta jezika, literarna pragmatika, lingvostilistika
Published in DiRROS: 25.11.2025; Views: 147; Downloads: 67
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342. Project management digitalisation of the clinical research at the University Medical Centre : good practice of using REDCap as a digitalisation toolZdenko Garaševič, Franc Strle, Martina Jaklič, 2025, original scientific article Abstract: Objective: The digital tool REDCap (Research Electronic Data Capture) was implemented at the University Medical Centre Ljubljana (UMCL) with the goal of digitalising and streamlining research processes. This study aimed to assess the efficiency and transparency of clinical research following the implementation of REDCap. Methods: The implementation of REDCap for funded research began in 2021. It comprised four key steps: (I) the initial creation of Central Research Registry, followed by additional functionalities including (II) the establishment of the Central Database for ’Pre-Contract Activities’ for clinical trials; (III) the integration of Reporting on Research Progress directly into the Central Research Registry; and (IV) the development of a semi-automated Workflow for internal agreements. Results: Between 2021 and 2023, UMCL established a Central Research Registry using REDCap, transitioning from paper-based to digital data collection for over 2,500 research projects. These projects included clinical trials, national and international studies, as well as academic research. In addition to serving as a registry, the central system provided comprehensive data management, streamlined communication, and enhanced collaboration among stakeholders in clinical trial research at UMCL. The implementation of REDCap significantly reduced administrative burden and shortened the time required to finalise clinical trial agreements from 202 to 147 days. It also improved coordination, transparency, and real-time monitoring of research activities, facilitating more efficient research execution. Additionally, the digitalisation of internal agreements processes between researchers and stakeholders within UMCL improved coordination and expedited research execution timelines. Furthermore, REDCap enabled real-time monitoring of research progress, further contributing to the efficiency and transparency of research activities. Conclusion: The digitalisation of research processes using REDCap improved the organisation and execution of research, leading to greater efficiency and transparency, reduced administrative workload, and enhanced collaboration. This approach contributed to higher-quality research outcomes and ultimately benefited patient care.
Keywords: digitalisation, REDCap, clinical research, efficiency, transparency, research registry, data management
Published in DiRROS: 24.11.2025; Views: 167; Downloads: 74
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343. A rapid method for determination of rosuvastatin in blood plasma with supported liquid extractionTjaša Dermota, Mojca Božič Mijovski, Jurij Trontelj, 2025, original scientific article Abstract: Introduction: Accurate measurement of rosuvastatin in plasma is critical for effective patient management and treatment monitoring following myocardial infarction (MI). Expensive solid-phase extraction (SPE) and timeconsuming liquid-liquid extraction (LLE) have been established for quantifying rosuvastatin. Supported liquid extraction (SLE) could offer a rapid, cost-effective alternative. Objectives: This study aimed to develop and validate a rapid, cost-effective, accurate, and precise method for quantifying rosuvastatin in high-dose plasma samples from patients following MI. Methods: Rosuvastatin was extracted from EDTA plasma using SLE and quantified with LC-MS/MS with positive electrospray ionization. The method was validated according to ICH M10 guidelines, focusing on selectivity, matrix effect, accuracy, precision, linearity, and carryover. Rosuvastatin-D6 was used as an internal standard. Additionally, thirty plasma samples from patients on high-dose rosuvastatin therapy (20 or 40 mg/day) following MI were analyzed by both LLE and SLE methods and compared. Results: The method was successfully validated, demonstrating linearity across a range of 0.1 ng/mL to 50 ng/mL. Compared to the LLE method, SLE achieved superior extraction recovery (96.3 % vs. 60 %) and precision (RSD: 11.9 % vs. 13.6 %) at 0.3 ng/mL rosuvastatin, with a lower absolute matrix effect (12.7 % vs.-36.7 %). Accuracy was comparable (109.3 % vs. 92.8 %). Although SLE involves higher initial costs, it significantly enhances throughput, reduces solvent usage, and minimizes contamination and equipment wear. Conclusion: This study validates SLE as a superior method for quantifying rosuvastatin in plasma, outperforming LLE in recovery, reproducibility, and automation. SLE offers greater accuracy and reliability, making it ideal for high-throughput applications.
Keywords: supported liquid extraction, liquid-liquid extraction, rosuvastatin, LC-MS/MS, myocardial infarction, solid-phase extraction, blood plasma
Published in DiRROS: 24.11.2025; Views: 158; Downloads: 60
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344. An evolutionarily conserved role for CTNNB1/ β-CATENIN in regulating the development of the corpus callosumArpan Parichha, Debarpita Datta, Amrita Singh, Ishita Talwar, Shreya Yadav, Špela Miroševič, Nina Žakelj, David Gosar, Damjan Osredkar, 2025, original scientific article Abstract: The corpus callosum (CC) is a major nerve bundle that connects the two hemispheres of the brain. Dysgenesis of the CC is associated with neurodevelopmental disorders such as the CTNNB1 syndrome. We identified that five individuals carrying CTNNB1 mutations displayed CC deficits. To explore CTNNB1/β-CATENIN-dependent mechanisms that regulate CC midline crossing, we examined mice with Ctnnb1 gain-of-function (GOF) or loss-of-function (LOF) selectively targeted to the early embryonic central nervous system midline using an Lmx1aCre driver. We identify that the Lmx1a lineage contributes to midline cell populations known to regulate CC pathfinding: the glial wedge, the indusium griseum glia, and a population of midline glutamatergic neurons. We find that each of these structures are affected in both GOF and LOF embryos, resulting in a profound disruption of CC crossing and formation of Probst bundles. Thus, regulated β-CATENIN function in midline cell populations is critical for CC development and its dysregulation may underlie the CC deficits associated with CTNNB1 syndrome.
Keywords: neurodevelopment, molecular neuroscience, CTNNB1 syndrome, corpus callosum development
Published in DiRROS: 24.11.2025; Views: 162; Downloads: 73
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