151. Modulation of the proteasome pathway by nano-curcumin and curcumin in retinal pigment epithelial cellsJ. Emanuel Ramos de Carvalho, Milan T. Verwoert, Ilse M.C. Vogels, Sabine Schipper-Krom, Cornelis J. F. van Noorden, Eric A. Reits, Ingeborg Klaassen, Reinier O. Schlingemann, 2018, original scientific article Abstract: Introduction: Curcumin has multiple biological effects including the modulation of protein homeostasis by the ubiquitin-proteasome system. The purpose of this study was to assess the in vitro cytotoxic and oxidative effects of nano-curcumin and standard curcumin and characterize their effects on proteasome regulation in retinal pigment epithelial (RPE) cells. Methods: Viability, cell cycle progression, and reactive oxygen species (ROS) production were determined after treatment with nano-curcumin or curcumin. Subsequently, the effects of nano-curcumin and curcumin on proteasome activity and the gene and protein expression of proteasome subunits PA28α, α7, β5, and β5i were assessed. Results: Nano-curcumin (5-100 μM) did not show significant cytotoxicity or anti-oxidative effects against H2O2-induced oxidative stress, whereas curcumin (≥10 μM) was cytotoxic and a potent inducer of ROS production. Both nano-curcumin and curcumin induced changes in proteasome-mediated proteolytic activity characterized by increased activity of the proteasome subunits β2 and β5i/β1 and reduced activity of β5/β1i. Likewise, nano-curcumin and curcumin affected mRNA and protein levels of household and immunoproteasome subunits. Conclusions: Nano-curcumin is less toxic to RPE cells and less prone to induce ROS production than curcumin. Both nano-curcumin and curcumin increase proteasome-mediated proteolytic activity. These results suggest that nano-curcumin may be regarded as a proteasome-modulating agent of limited cytotoxicity for RPE cells.
Keywords: curcumin, nano-curcumin, proteasome, retinal pigment epithelium
Published in DiRROS: 26.02.2025; Views: 108; Downloads: 54
 Full text (737,80 KB)
This document has many files! More... |
152. Solitary ovarian cancer cells in the peritoneum : what happens below the surface?Laura M.C. Vos, Willemien J. van Driel, Gabe S. Sonke, Juliette O. A. M. van Baal, Koen K. van de Vijver, Cornelis J. F. van Noorden, Christianne A. R. Lok, 2022, original scientific article Abstract: Background
In advanced epithelial ovarian cancer (EOC), the peritoneum is the primary site of disease recurrence which occurs in >75% of patients despite complete cytoreductive surgery (CRS) and chemotherapy. Macroscopically undetectable remaining cancer cells are deemed to be a source for recurrent disease. We investigated characteristics of occult disease in biopsies of macroscopically normal peritoneum during CRS.
Materials and methods
We included 14 patients with advanced stage high grade serous ovarian cancer (HGSOC). Eleven patients had received neoadjuvant chemotherapy (NACT) and three patients were chemotherapy naïve. Each patient underwent three study-related peritoneal biopsies: 1) of a metastasis, 2) adjacent to a metastasis and 3) at distance from metastases. Cryostat sections were immunohistochemically stained for PAX8 and PanCK as markers of EOC cells and for CD31 as a marker for vascular and lymphatic endothelium. The sections were analyzed semi-quantitatively.
Results
Macroscopically normal peritoneum showed solitary PAX8-positive cells adjacent to and at distance from metastases in all patients. Thirteen percent of these PAX8-positive cells were found to be attached to the mesothelium and are presumably spread through intra-abdominal fluid. Eighty-seven percent of the solitary PAX8-positive cells were found in the stroma underneath the mesothelium, of which 59% were firmly attached to endothelium and 33% were found in the stroma. In most cases, no sign of proliferation of the solitary cells was observed. Only a few clusters of PAX8-positive cells were found. Chemotherapy did not affect these results.
Conclusions
Solitary PAX8-positive cells are present in the macroscopically healthy-looking peritoneum of all EOC patients investigated, irrespective of the distance to macroscopically-visible metastases and of previous treatment. The majority of these solitary cancer cells were attached to endothelium of capillaries, venules or lymphatic vessels. Their solitary character and lack of proliferation suggests a dormant state, which could explain why these cells are unaffected by neo-adjuvant chemotherapy.
Keywords: ovarian cancer, peritoneal metastasis, translational medical research, human pathology, PAX8, cancer recurrence
Published in DiRROS: 26.02.2025; Views: 119; Downloads: 95
Full text (5,53 MB)
This document has many files! More... |
153. |
154. Application of non-destructive MARVIN and NIR seed screening of pre-breeding material: A case study on common bean (Phaseolus vulgaris), white lupin (Lupinus albus) and Andean lupin (L. mutabilis)Barbara Pipan, Lovro Sinkovič, Mohamed Neji, Vladimir Meglič, Karolina Susek, Magdalena Kroc, Elisa Bellucci, Elena Bitocchi, Roberto Papa, 2025, original scientific article Keywords: legumes, NIR, spectroscopy, plant genetic resources, pre-breeding collection, protein content, seed traits
Published in DiRROS: 25.02.2025; Views: 117; Downloads: 50
Full text (1,02 MB)
This document has many files! More... |
155. Prevalence of key resistance alleles associated with DMI and SDHI fungicide resistance in European Zymoseptoria tritici populations in 2022Stephen Kildea, Thies Marten Heick, Fiona Hutton, Charlotte Bataille, Louise Aldén, Janis Kaneps, Andres Mäe, Stephan Weigand, Janja Zajc Žunič, Anne-Sophie Walker, P. Hellin, 2025, original scientific article Keywords: Mycosphaerella graminicola, sensitivity, disease control, Septoria tritici blotch, triazole, wheat
Published in DiRROS: 25.02.2025; Views: 123; Downloads: 79
Full text (1,55 MB)
This document has many files! More... |
156. |
157. Thermal deformation behavior of high-purity rare earth metal dysprosiumHaishuang Lv, Yanmei Li, Dounan Chen, Guohuai Liu, Rui Liu, Zhaodong Wang, 2025, original scientific article Keywords: dysprosium, hot deformation behavior, stress-strain curve, processing maps
Published in DiRROS: 21.02.2025; Views: 128; Downloads: 62
Full text (4,76 MB)
This document has many files! More... |
158. Component optimization of g−C3N4/TiO2 hetero-structure composite and its activity enhancement for photocatalytic degradation of rhodamine BZikang Cao, Yating Liu, Luqi Wang, Xu Gao, Sitian Chen, Sitian Cheng, Yan Yu, Li Li, 2025, original scientific article Keywords: hydrothermal synthesis, g-C3N4, TiO2, photocatalysis
Published in DiRROS: 21.02.2025; Views: 125; Downloads: 64
Full text (6,44 MB)
This document has many files! More... |
159. Hydrothermal synthesis and characterization of ZSM-5 zeolite from anorthosite: impacts of reaction time and temperatureYonas Desta Bizualem, Mudasir Akbar Shah, Soumaya Ibrahimi, Aicha Gasmi, Noureddine Elboughdiri, 2025, original scientific article Keywords: anorthosite, zeolite, hydrothermal synthesis, ZSM-5
Published in DiRROS: 21.02.2025; Views: 111; Downloads: 57
Full text (2,99 MB)
This document has many files! More... |
160. Research on the water-holding capacity and mechanical properties of backfill based on a damage-softening constitutive model: effects of different permeability forcesChaoyi Yang, Zhenggao Huang, Wenzhong Sha, Heng Huang, Rugao Gao, 2025, original scientific article Keywords: backfill, centrifugal simulation, osmotic pressure, mechanical properties
Published in DiRROS: 21.02.2025; Views: 103; Downloads: 54
Full text (5,71 MB)
This document has many files! More... |
