1291. On mechanisms of cell plasma membrane vesiculationVeronika Kralj-Iglič, Urška Batista, Henry Hägerstrand, Aleš Iglič, Janja Majhenc, Mihael Sok, 1998, original scientific article Keywords: budding, membrane bilayer, vesiculation Published in DiRROS: 19.01.2024; Views: 246; Downloads: 79
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1294. Vinblastine increases antitumor effectiveness of bleomycinMaja Čemažar, Marija Auersperg, Gregor Serša, 1997, original scientific article Abstract: In our previous vinblastine (VELBE) was shown to increase the plasma membrane fluidity. This effect of VELBE might be expolited for better transport of drugs through the plasma membrane. Bleomycin (BLM) is a highly cytotoxic drug when present inside the cells but has a hampered transport through the plasma membrane. The aim of the present study was to determine whether pretreatment with VELBE can increase the effect of BLM on intraperitoneal SA-1 tumors in mice. BLM and VELBE were used as single agents or in various combinations, i.e. VELBE and BLM injected simultanously, BLM injected 24 h before VELBE or VELBE injected 24 h before BLM. Mice survival was the end-point used for determining the effect of this combined treatments. VELBE and BLM as a single treatment significantly prolonged median survival time of study animals compared to controls. Furthermore, when VELBE and BLM were combined, all threetested combinations were more effective than VELBE or BLM as single treatments. The effect on animal survival was equal when VELBE was given 24 h after or simultanously with BLM. The longest survival, however was obtained when VELBE was injected 24 h before BLM. From these results we can conclude that the underlying mechanisms for more than additive effect of VELBE and BLM when VELBE was given 24 h before BLM could be attributed to an increased membrane fluidity, possibly in combination with a cell kinetic effect. Published in DiRROS: 18.01.2024; Views: 241; Downloads: 61
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1295. Long term follow-up after radiosynovectomy with yttrium 90 in patients with different rheumatic diseasesMojca Golja, Nataša Budihna, Igor Batagelj, 1997, original scientific article Abstract: The aim of the retrospective study was to evaluate the efficacy of radiosynovectomy (with yttrium 90) mainly in patients with rheumatoid arthritis, less with some other rheumatic diseases. The evaluation period varied from half to nine years. The procedure was performed in 273 patients (225 females, 48 males) or in 463 joints (402 knees, 61 shoulders and ankles).The effects was evaluated by change in degree of morning stiffiness, pain and swelling (score from 0 to 9). Very good results were obtained in 69 (15%), good in 142 (30.5%), moderate in 197 (42.5%) and no effect in 55 (12%) joints. Six months after the procedure 38 joints (8%), half to two years after221 joint (48%) were in good remission, after 3 to 4 years 95 joints (20%), after 5 to 6 years 57 joints (12%) were well, 7 to 9 years later 52 joints (11%) showed no signs of arthritis. Joint pain and swelling were the most frequent procedure complications (5.6%). In two patients with additional immunomodulating therapy chronic myeloid and lymphocityc leukaemia were diagnosed. Radiosynovectomy is considered to be an effective and safe treatment for synovitis indifferent rheumatic diseases. Published in DiRROS: 18.01.2024; Views: 331; Downloads: 70
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1296. Cytology of mediastinal tumorsMilivoj Mermolja, Izidor Kern, Marjeta Terčelj-Zorman, Marjan Jereb, 1997, original scientific article Abstract: Our experience with cytological examinations of tumorous mediastinal lesions is evaluated. A group of 117 patients with mediastinal tumor have been included into the study. Among them carcinomas prevailed (60,7%), followed by lymphomas (18,8%), other tumors (15.4%) and thymic neoplasms (5.1%). Malignantor suspicious cells were found in 77.4% of patients with carcinoma. The cells indicating a possibility of non-Hodgkin's lymphoma were found in 9 out of 14 patients. In 5 out of 6 thymic neoplasms the cytological pattern wasconsistent with the diagnosis of thymic neoplasm. One case of thymoma was cytologically falsely diagnosed as malignant lymphoma. One case of neurofibroma was falsely diagnosed as adenocarcinoma. The sensitivity of cytological examinations was 67.5%. If 18 patients with diagnostically unsatisfactory material were excluded from the analysis, the sensitivity wouldincrease to 80.8%. Owing to the wide variety of primary and metastatic tumors that can occur in the mediastinum, apart from the routine cytological techniques, additional staining methods should be used. For final cytological diagnosis the integration of cytological findings with clinical and radiological data is often required. Owing to the characteristics of the obtained material and biological behaviour of some mediastinal tumors, some tumors cannot be definitively diagnosed by cytological examinations alone. Published in DiRROS: 18.01.2024; Views: 349; Downloads: 86
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1298. A role of gender in the occurrence of dimethylhydrazine induced colorectal tumors in Wistar ratsLjubo Breskvar, Anton Cerar, 1997, original scientific article Abstract: Human colorectal carcinoma appears more frequently in males. The aim of our study was to evaluate the influence of gender on the induction of colorectal carcinoma by 1,2-dimethylhydrazine (DMH) in Wistar rats. Sixty Wistar rats (30males, 30 females) were subjected to weekly subcutanous injections of DMH (20 mg/kg) for 15 weeks. After 25 weeks from the beginning of the experiment the animals were sacrificed and autopsied. All macroscopical lesions were evaluated histologicaly. Induction of colorectal tumors succeeded in 37% of males and 17% of females. There were 21 tumors of the large bowel found, of these 15 in males and 6 in females. Histologically, males had 11 adenomas, 2 signet-cell carcinomas and 2 adenocarcinomas, while females had 4 signet-cell carcinomas and 2 adenomas. We also found extracolonic tumors, mainly those of the small intestine and of the Zymbal glands. Wistar rats showed lower incidence of DMH-induced colorectal tumors in comparison with other strains ofrats. The gender-dependent difference in the incidence of colorectal tumors was found to be statistically marginally significant (p<0.08), whereas the difference in the incidence of all induced tumors between genders was significant (p<0.02). Males showed a greater incidence of coloreactal tumors and also a greater histological resemblance to human colorectal tumors than females. That is why we recommend Wistar males rather than females for research on colorectal tumors. Published in DiRROS: 18.01.2024; Views: 289; Downloads: 60
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1299. Interferon alpha (IFN-[alpha]) in treatment of malignant diseasesBerta Jereb, 1997, review article Abstract: There are several natural varieties of IFN-alpha in clinical use. Although IFNs were the first cytokines in use as a new treatment modality, we still know little about their mode of action in malignancies. IFN-alpha can inhibit cell growth, but it has been shown only recently, on malignant cells from patients with multiple myeloma, that IFN-alpha can exert a direct cytotoxic effect on tumor cells. The treatment with natural leucocyte IFN-alpha in dosesused in the early clinical trials, are still most widely used. It has been shown that through a high local concentration by local application in malignant melanoma, basalioma, pleural carcinosis, glioblastoma, total local tumor control may be achieved. it is thys not known what the optimal doses of IFN-alpha are, and they may vary by the tumor type, as well as from patient topatient. The neccessary duration of treatment also remains an open question.IFNs are a tool for treatment of malignant tumors. Their cytotoxic effect, however, is not strong enough and does not last. At present, there is a vast field of investigative work stil open-but in the meantime it seems necessary to proceed with clinical trials of different types of IFN, the natural IFN-alpha again beeing the most interesting one. Published in DiRROS: 18.01.2024; Views: 262; Downloads: 56
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