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Abstract: Background. Glioblastoma (GBM) is the most common and the most malignant glioma subtype. Among numerous genetic alterations, miRNAs contribute to pathogenesis of GBM and it is suggested that also to GBM recurrence and resistance to therapy. Based on publications, we have selected 11 miRNAs and analyzed their expression in GBM. We hypothesized that selected miRNAs are differentially expressed and involved in primary as well as in recurrent GBM, that show significant expressional differences when different treatment options are in question, and that are related to certain patients and tumor characteristics. Patients and methods. Paraffin embedded tissues, obtained from primary and corresponding recurrent tumor from 83 patients with primary GBM were used. Eleven miRNAs ( miR-7, miR-9, miR-15b, miR-21, miR-26b, miR-124a, miR-199a, let-7a, let-7b, let-7d, and let-7f ) were selected for qPCR expression analysis. For patients who received temozolamide (TMZ) as chemotherapeutic drug, O6-methylguanine-DNA methyltransferase (MGMT) methylation status was defined using the methyl-specific PCR. Results. There was a significant change in expression of miR-7, miR-9, miR-21, miR-26b, mirR-124a, miR-199a and let- 7f in recurrent tumor compared to the primary. In recurrent tumor, miR-15b, let-7d and let-7f significantly changed comparing both treatment options. We also observed difference in progression free survival between patients that received radiotherapy and patients that received radiotherapy and chemotherapy, and longer survival for patients who received chemotherapy after second surgery compared to not treated patients. miR-26b showed correlation to progression free survival and let-7f to overall survival. We did not find any expression difference between the tumors with and without methylated MGMT. Conclusions. Our data suggest that analyzed miRNAs may not only contribute to pathogenesis of primary GBM, but also to tumor progression and its recurrence. Moreover, expression of certain miRNAs appears to be therapy- dependent and as such they might serve as additional biomarker for recurrence prediction and potentially predict a therapy-resistance.
Keywords: glioblastoma, radiotherapy, chemotherapy
Published in DiRROS: 02.07.2024; Views: 313; Downloads: 186
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Abstract: Background. Sclerosing melanocytic lesions, which are characterized by either focal or diffuse sclerosis in the dermal component and atypical proliferation of predominantly nevoid melanocytes, remain poorly defined. Our aim was to analyze systematically their morphologic spectrum, especially the distinction between sclerosing melanocytic nevus and sclerosing melanoma, which has not been well documented. Patients and methods. We collected 90 sclerosing melanocytic lesions, occurring in 82 patients (49 male, 33 female; age range from 21 to 89 years). A four probe fluorescent in situ hybridization (FISH) assay was performed in 41 lesions to substantiate the diagnosis of sclerosing melanomas. Results. A prominent full-thickness pagetoid spread of melanocytes was identified in 44 (48%) lesions, and a melanoma in situ adjacent to the sclerosis in 55 (61%) lesions. In the intrasclerotic component, maturation was absent in 40 (44%) and mitotic figures were identified in 18 (20%) lesions. Of the 90 lesions, 26 (29%) were diagnosed morphologically as nevi and 64 (71%) as melanomas (Breslow thickness from 0.4 to 1.8 mm), including 45 (50%) melanomas with an adjacent nevus. A four-probe FISH assay was positive in the sclerotic component in 14 of 25 lesions diagnosed morphologically as melanomas and none of 16 nevi. A sentinel lymph node biopsy was performed for 17 lesions and was negative in all cases. Conclusions. Sclerosing melanocytic lesions form a morphologic spectrum and include both nevi and melanomas. The pathogenesis of sclerosis remains obscure but seems to be induced by melanocytes or an unusual host response in at least a subset of lesions.
Keywords: sclerosing melanoma, sclerosing nevus, fibrosis
Published in DiRROS: 02.07.2024; Views: 341; Downloads: 236
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Keywords: vegetacija, tla, rastišča, ekologija gozdov, Javna gozdarska služba, Slovenija
Published in DiRROS: 02.07.2024; Views: 383; Downloads: 194
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Keywords: vegetacija, tla, rastišča, ekologija gozdov, Javna gozdarska služba, Slovenija
Published in DiRROS: 02.07.2024; Views: 365; Downloads: 170
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Abstract: The basic idea was to create a proof of concept demonstrating that the indoor navigation and localisation is possible using only passive tags. For this purpose, a smartphone navigation app was developed to be used in inner parts of the buildings and which can operate with no satellite positioning service available and no communication network present. The satellite navigation systems (GPS, GLONASS, Galileo, BeiDou, QZSS, IRNSS) inside buildings, at least on lower floors and cellars, in central parts, and away from windows, usually do not work, or their accuracy is very reduced due to a small number of visible satellites. The communication networks (WiFi, LTE, 5G…) might not be available in some circumstances such as catastrophic incidents, power reductions, or similar, which disables the localisation systems based on them. The purpose of the smartphone app was twofold. First, to develop a reliable, simple-to-use, and cheap indoor navigation system that could be used in large buildings like hospitals, shopping malls, trade centres, fairs, etc. where no other positioning service is available. Second, to develop an indoor position reporting system that can be used in accidents and mass-casualty incidents for reporting triage decisions to the server. Both functionalities are based on the QR codes [1] holding all the information needed.
Keywords: internet thinks, indoor navigation, systems, QR codes
Published in DiRROS: 02.07.2024; Views: 296; Downloads: 190
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