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474. Multiplex quantification of 19 GM soybean lines using digital PCRAmadej Jelenčič, Dejan Štebih, Tina Demšar, David Dobnik, 2026, independent scientific component part or a chapter in a monograph Abstract: The European Union (EU) imposes strict regulations on the presence of genetically modified (GM) material in food and feed, requiring thorough testing of samples for various GM lines. Although traditional quantitative real-time PCR (qPCR) methods are sensitive and robust, they are not cost-effective for managing large numbers of GM events due to their limited multiplexing capabilities. Conversely, digital PCR (dPCR) is capable of robust quantitative multiplexing in addition to other benefits such as absolute quantification and better tolerance of PCR inhibitors. In this context, we present a protocol for multiplex quantification of 19 GM soybean lines using dPCR as an improvement over the currently used simplex qPCR approach. This method enables simple and robust quantification of common GM soybean lines with a relatively low number of reactions.
Keywords: genetically modified soybean, GMO, multiplexing, food and feed, food regulation, digital PCR (dPCR)
Published in DiRROS: 26.11.2025; Views: 134; Downloads: 47
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475. Strain-controlled superconductivity in epitaxially grown thin films of ▫$1T-TaS_2$▫Yelyzaveta Chernolevska, Anže Mraz, Rok Venturini, Bojan Ambrožič, Tomaž Mertelj, Goran Dražić, Damjan Svetin, Damjan Vengust, Hsin-Chia Ho, Matjaž Spreitzer, Dragan Mihailović, 2025, original scientific article Keywords: transition metal dichalcogenides, thin films, molecular beam epitaxy
Published in DiRROS: 26.11.2025; Views: 152; Downloads: 83
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476. The European Information System on Forest Genetic Resources (EUFGIS): : a transnational tool for genetic conservation and policy supportMichele Bozzano, Valentina Barbiero, Egbert Beuker, Simone Mori, Mari Rusanen, Ivan Scotti, Milko Skofic, Marjana Westergren, 2025, published scientific conference contribution abstract Keywords: forest genetic resources, information system, Europe
Published in DiRROS: 26.11.2025; Views: 123; Downloads: 62
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477. Trans fatty acids in margarines and shortenings in the food supply in SloveniaHelena Abramovič, Rajko Vidrih, Emil Zlatić, Doris Kokalj Sinkovič, Matthias Schreiner, Katja Žmitek, Anita Kušar, Igor Pravst, 2018, original scientific article Published in DiRROS: 26.11.2025; Views: 180; Downloads: 94
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478. Signalling network construction for modelling plant defence responseDragana Miljković, Tjaša Stare, Igor Mozetič, Vid Podpečan, Marko Petek, Kamil Witek, Marina Dermastia, Nada Lavrač, Kristina Gruden, 2012, original scientific article Published in DiRROS: 26.11.2025; Views: 187; Downloads: 108
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479. In vitro evaluation of electrochemotherapy combined with sotorasib in pancreatic carcinoma cell lines harboring distinct kras mutationsTanja Jesenko, Maša Omerzel, Tina Živič, Gregor Serša, Maja Čemažar, 2025, original scientific article Abstract: Pancreatic cancer is among the deadliest malignancies, with limited treatment options and poor prognosis. Novel strategies are therefore urgently needed. Sotorasib, a KRAS G12C-specific inhibitor, offers targeted treatment for a small subset of patients with this mutation. Electrochemotherapy (ECT), which enhances the cytotoxicity of chemotherapeutic agents through electroporation-induced membrane permeabilization, has shown promise in various tumor types, including deep-seated malignancies such as pancreatic cancer. Combining ECT with sotorasib may potentiate antitumor effects in KRAS G12C-mutated pancreatic cancer; however, preclinical data on such combinations are lacking. This proof-of-concept study evaluated the cytotoxic effects of ECT using bleomycin (BLM) or cisplatin (CDDP) in combination with sotorasib in KRAS G12C-mutated MIA PaCa-2 and KRAS G12D-mutated PANC-1 pancreatic cancer cell lines. ECT alone significantly reduced cell viability, particularly in MIA PaCa-2 cells, where electric pulses induced approximately 75% cell death. Combining ECT with sotorasib resulted in an additive effect on KRAS G12C-mutated MIA PaCa-2 cells, though no synergy was observed, likely due to the high intrinsic sensitivity to electric pulses. These results support the potential of combining physical and molecular therapies in a subset of pancreatic cancer patients and lay the groundwork for further in vivo studies to optimize treatment parameters and explore clinical translatability.
Keywords: bleomycin, cisplatin, electrochemotherapy, pancreatic cancer
Published in DiRROS: 26.11.2025; Views: 112; Downloads: 51
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