1. Evaluation of the training program for p16/ Ki-67 dual immunocytochemical staining interpretation for laboratory staff without experience in cervical cytology and immunocytochemistryVeronika Kloboves-Prevodnik, Živa Pohar-Marinšek, Janja Zalar, Hermina Rozina, Nika Kotnik, Tine Jerman, Jerneja Varl, Urška Ivanuš, 2020, original scientific article Abstract: ackground p16/Ki-67 dual immunocytochemical staining (DS) is considered easy to interpret if evaluators are properly trained, however, there is no consensus on what constitutes proper training. In the present study we evaluated a protocol for teaching DS evaluation on students inexperienced in cervical cytology. Methods Initial training on 40 DS conventional smears was provided by a senior cytotechnologist experienced in such evaluation. Afterwards, two students evaluated 118 cases. Additional training consisted mainly of discussing discrepant cases from the first evaluation and was followed by evaluation of new 383 cases. Agreement and accuracy of students' results were compared among the participants and to the results of the reference after both evaluations. We also noted time needed for evaluation of one slide as well as intra-observer variability of the teacher's results. Results At the end of the study, agreement between students and reference was higher compared to those after initial training (overall percent agreement [OPA] 81.4% for each student, kappa 0.512 and 0.527 vs. OPA 78.3% and 87.2%, kappa 0.556 and 0.713, respectively). However, accuracy results differed between the two students. After initial training sensitivity was 4.3% points and 2.9% points higher, respectively compared to the reference, while specificity was 30.6% points and 24.4% points lower, respectively, compared to the reference. At the end of the study, the sensitivity reached by one student was the same as that of the reference, while it was 2.6% points lower for the other student. There was a statistically significant difference in specificity between one student and the reference and also between students (16.7 and 15.1% points). Towards the end of the study, one student needed 5.2 min for evaluating one slide while the other needed 8.2 min. The intra-observer variability of the senior cytotechnologist was in the range of "very good" in both arms of the study. Conclusions In teaching DS evaluation, the students' progress has to be monitored using several criteria like agreement, accuracy and time needed for evaluating one slide. The monitoring process has to continue for a while after students reach satisfactory results in order to assure a continuous good performance. Monitoring of teacher's performance is also advisable. Keywords: cervical cytology, cervical cancer, immunocytochemistry, accuracy Published in DiRROS: 11.07.2024; Views: 168; Downloads: 145
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2. Clinical relevance of the borderline results of the Hybrid Capture 2 High-Risk HPV DNA assay with cervical samples collected in Specimen Transport MediumJerneja Varl, Urška Ivanuš, Živa Pohar-Marinšek, Tine Jerman, Anja Oštrbenk Valenčak, Mario Poljak, Veronika Kloboves-Prevodnik, 2019, original scientific article Keywords: Hybrid Capture 2, cervical cancer, HPV test, borderline results Published in DiRROS: 05.07.2024; Views: 186; Downloads: 98
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3. Evolving strategies in the treatment of childhood rhabdomyosarcoma : Slovenian experienceŽiva Pohar-Marinšek, Jožica Anžič, Berta Jereb, 2001, original scientific article Abstract: Background. Neoadjuvant chemotherapy (Cht) has changed the treatment of rhabdomyosarcoma (RMS) in children. The purpose of our study was to review thechildren treated for RMS between 1974 and 1996. Patients and methods. Fifty-one children, 1-15 years old, were included. Primary sites of tumour were: head and neck 15, orbit 6, genitourinary 12, extremity 9, torso 5 and paratesticular 4. Twelve patients were in stage 1, 10 in stage II, 26 in stage111 and 3 in stage IV. Of 43 histologically confirmed RMS 25 were embryonal, 13 alveolar, 1 botryoid, 1 spindle cell and 3 sarcoma NOS. In 8 patients, only fine needle aspiration biopsy (FNAB) was available. All patients had Cht, 29 neoadjuvant, 20 had surgery first, 40 had irradiation (RT), 2 stage IV patients had bone marrow transplant (ABMT). Multidrug Cht varied: VCR, AMD, and cyclophosphamide (VAC) were used in the 1970s, with Adriablastine (T2), methotrexat (MTX) and/or other drugs (T6, T11) in the 1980s; and in the 1990s, cyclophosphamide was replaced by ifosfamide (VAIA). The treatment was started with Cht in orbital and head and neck tumours and inthe majority of genitourinary tumours, but surgery was first in paratesticular and in the majority of extremity tumours. Results. The 3 patients with stage IV disease died. Of those with localised tumour, 34 (70%) were alive and well 5 years after treatment, 80% stage I, 75% stage II and 61%stage III. One patient died of heart failure, 3 of Cht toxicity and 1 of intereurrent disease. Conclusions. (Abstract truncated at 2000 characters) Published in DiRROS: 26.01.2024; Views: 316; Downloads: 85
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4. Resections of pelvic bone and sacrum, Ljubljana experienceJanez Novak, Marjana Čuček-Pleničar, Darja Eržen, Franc Srakar, Mojca Senčar, Boštjan Baebler, Jožica Červek, Alenka Vodnik-Cerar, Matej Bračko, Živa Pohar-Marinšek, Jožica Anžič, 1997, original scientific article Published in DiRROS: 16.01.2024; Views: 386; Downloads: 102
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5. Navodila ginekologom za delo v programu ZORA : posodobitev 2011Maja Primic-Žakelj, Marjetka Uršič-Vrščaj, Ana Pogačnik, Urška Ivanuš, Andrej Možina, Živa Pohar-Marinšek, Mario Poljak, 2011, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: citodiagnostika, presejanje, papiloma virusi, kolposkopija, predrakave spremembe, diagnostika, zdravljenje, zborniki Published in DiRROS: 04.06.2020; Views: 1988; Downloads: 707
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6. Navodila za poenotenje izvidov brisov materničnega vratuAna Pogačnik, Irena Srebotnik-Kirbiš, Alenka Repše-Fokter, Živa Pohar-Marinšek, Vivijana Snoj, Irena Kirar-Fazarinc, Maja Primic-Žakelj, 2005, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: bris, citopatologija Published in DiRROS: 04.06.2020; Views: 2043; Downloads: 613
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7. Smernice diagnostike in zdravljenja diferenciranega raka ščitniceNikola Bešić, Andreja A. Schwarzbartl-Pevec, Andreja Klevišar Ivančič, Barbara Vidergar-Kralj, Barbara Gazić, Blaž Krhin, Branko Zakotnik, Cvetka Grašič-Kuhar, Damijan Bergant, Edvard Pirnat, Helena Gutnik, Ivana Žagar, Jerca Blazina, Katja Zaletel, Margareta Strojan Fležar, Marko Hočevar, Marta Dremelj, Nebojša Glumac, Olga Blatnik, Simona Gaberšček, Snežana Pavlović Djokić, Ulrika Klopčič, Veronika Kloboves-Prevodnik, Živa Pohar-Marinšek, 2017, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: rak ščitnice, diferencirani rak, zdravljenje, smernice Published in DiRROS: 27.05.2020; Views: 2179; Downloads: 705
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9. Atipične žlezne celice, neopredeljene : rezultati revizije citoloških preparatov in tveganje CIN2+Veronika Kloboves-Prevodnik, Simona Uhan Kastelic, Živa Pohar-Marinšek, Tina Žagar, Urška Ivanuš, 2014, published scientific conference contribution Keywords: bris materničnega vratu, atipične žlezne celice, lezije Published in DiRROS: 08.04.2020; Views: 2574; Downloads: 722
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