1. Cytotoxicity and antibacterial efficacy of betaine- and choline-substituted polymersLucija Jurko, Damjan Makuc, Alja Štern, Janez Plavec, Bojana Žegura, Perica Bošković, Rupert Kargl, 2023, original scientific article Abstract: Cationic charge has been widely used to increase polymer adsorption and flocculation of dispersions or to provide antimicrobial activity. In this work, cationization of hydroxyethyl cellulose (HEC) and polyvinyl alcohol (PVA) was achieved by covalently coupling betaine hydrochloride and choline chloride to the polymer backbones through carbonyl diimidazole (CDI) activation. Two approaches for activation were investigated. CDI in excess was used to activate the polymers’ hydroxyls followed by carbonate formation with choline chloride, or CDI was used to activate betaine hydrochloride, followed by ester formation with the polymers’ hydroxyls. The first approach led to a more significant cross-linking of PVA, but not of HEC, and the second approach successfully formed ester bonds. Cationic, nitrogen-bearing materials with varying degrees of substitution were obtained in moderate to high yields. These materials were analyzed by Fourier transform infrared spectroscopy, nuclear magnetic resonance, polyelectrolyte titration, and kaolin flocculation. Their dose-dependent effect on the growth of Staphylococcus aureus and Pseudomonas aeruginosa, and L929 mouse fibroblasts, was investigated. Significant differences were found between the choline- and betaine-containing polymers, and especially, the choline carbonate esters of HEC strongly inhibited the growth of S. aureus in vitro but were also cytotoxic to fibroblasts. Fibroblast cytotoxicity was also observed for betaine esters of PVA but not for those of HEC. The materials could potentially be used as antimicrobial agents for instance by coating surfaces, but more investigations into the interaction between cells and polysaccharides are necessary to clarify why and how bacterial and human cells are inhibited or killed by these derivatives, especially those containing choline.
Keywords: hydroxyethyl cellulose, polyvinyl alcohol, antimicrobial, S. aureus, P. aeruginosa, L929 mouse fibroblast, cationic polymer
Published in DiRROS: 12.07.2024; Views: 60; Downloads: 35
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2. Guanidine modification improves functions of natural RNA-targeting alkaloidsTamaki Endoh, Sagar Satpathi, Yutong Chen, Saki Matsumoto, Tatsuya Ohyama, Peter Podbevšek, Janez Plavec, Kazumitsu Onizuka, Fumi Nagatsugi, Naoki Sugimoto, 2024, original scientific article Published in DiRROS: 10.06.2024; Views: 103; Downloads: 58
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3. Protein G-quadruplex interactions and their effects on phase transitions and protein aggregationBikash Ranjan Sahoo, Vojč Kocman, Nathan Clark, Nikhil Myers, Xiexiong Deng, Ee L. Wong, Harry J. Yang, Anita Kotar, Bryan B. Guzman, Daniel Dominguez, Janez Plavec, James C. A. Bardwell, 2024, original scientific article Published in DiRROS: 03.06.2024; Views: 174; Downloads: 160
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4. Structure of native four-repeat satellite III sequence with non-canonical base interactionsErin Chen, Marko Trajkovski, Hyun Kyung Lee, Samantha Nyovanie, Kailey N. Martin, William L. Dean, Mamta Tahiliani, Janez Plavec, Liliya A. Yatsunyk, 2024, original scientific article Published in DiRROS: 30.04.2024; Views: 242; Downloads: 224
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10. Insight into tetramolecular DNA G-quadruplexes associated with ALS and FTLD : cation interactions and formation of higher-ordered structureMatja Zalar, Baifan Wang, Janez Plavec, Primož Šket, 2023, original scientific article Keywords: ALS, FTLD, DNA, c9orf7, NMR, quadruplex, cations
Published in DiRROS: 06.09.2023; Views: 411; Downloads: 247
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