1. Digital PCR for the characterization of reference materialsMegan H. Cleveland, Hua-Jun He, Mojca Milavec, Young-Kyung Bae, Peter M. Vallone, Jim F. Huggett, 2024, review article Abstract: Well-characterized reference materials support harmonization and accuracy when conducting nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non-target sequences, making it ideal for the characterization of nucleic acid-based reference materials. National Metrology Institutes are increasingly using dPCR to characterize and certify their reference materials, as it offers several advantages over indirect methods, such as UV-spectroscopy. While dPCR is gaining widespread adoption, it requires optimization and has certain limitations and considerations that users should be aware of when characterizing reference materials. This review highlights the technical considerations of dPCR, as well as its role when developing and characterizing nucleic acid-based reference materials.
Keywords: digital PCR, dPCR, reference materials, UV-spectroscopy
Published in DiRROS: 03.06.2024; Views: 3; Downloads: 0
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2. Poročilo o preskusu št.: LVG 2024-034 : vzorec št. 2024/00084Tine Hauptman, Špela Hočevar, Zina Devetak, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Euwallacea fornicatus, Neocosmospora euwallaceae, strokovne naloge IVHVVR, PCR
Published in DiRROS: 23.05.2024; Views: 83; Downloads: 56
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3. Poročilo o preskusu št.: LVG 2024-043 : vzorec št. 2024/00061Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 23.05.2024; Views: 86; Downloads: 0
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4. Poročilo o preskusu št.: LVG 2024-033 : vzorec št. 2024/00083Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 23.05.2024; Views: 73; Downloads: 0
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5. Poročilo o preskusu št.: LVG 2024-027 : vzorec št. 2024/00058Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 23.05.2024; Views: 73; Downloads: 0
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6. Poročilo o preskusu št.: LVG 2024-026 : vzorec št. 2024/00064Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 23.05.2024; Views: 73; Downloads: 0
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7. Poročilo o preskusu št.: LVG 2024-031 : vzorec št. 2024/00074 in 2024/00076Nikica Ogris, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR v realnem času, Diplodia pinea
Published in DiRROS: 19.04.2024; Views: 143; Downloads: 0
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8. Poročilo o preskusu št.: LVG 2024-030 : vzorec št. 2024/00068Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 19.04.2024; Views: 151; Downloads: 0
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9. Poročilo o preskusu št.: LVG 2024-029 : vzorec št.12136414Nikica Ogris, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Geosmithia morbida, bolezen tisočerih rakov, Juglans, PCR v realnem času
Published in DiRROS: 19.04.2024; Views: 121; Downloads: 0
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10. Poročilo o preskusu št.: LVG 2024-028 : vzorec št.10132326Tine Hauptman, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, program preiskav, Fusarium circinatum, Pinus, borov smolasti rak, PCR
Published in DiRROS: 19.04.2024; Views: 136; Downloads: 0
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