881. Subchronic exposure of rats to sublethal dose of microcystin-YR induces DNA damage in multiple organsMetka Filipič, Bojana Žegura, Bojan Sedmak, Irena Horvat-Žnidaršič, Aleksandra Milutinović Živin, Dušan Šuput, 2007, original scientific article Abstract: Background. Microcystins (MCs) are cyclic heptapeptides that are considered tobe liver specific toxins. They are potent tumour promoters and recent studies indicate that they are also genotoxic. In this study we measured DNA damage in lymphocytes, liver, kidney (cortex and medulla), lung, spleen and brain cells of male Fisher F344 rats that were exposed to sublethal dose (every second day 10 Ugžkg b.w.č i.p) of microeysrin-YR (MCYR) for one month. Methods. At the end of exposure the animals were sacrificed, the lymphocytes were isolated from blood taken from jugular vein, liver cells were obtained byperfusion with collagenase A and the cells from other organs were isolated by incubating small tissue pieces with eollagenase A. The DNA damage in isolated cells was measured with the single cells gel electrophoresis (SCGE) also called the comet assay. Results. A significant increase of the % tail DNAin MCYR-exposed animals compared to the nonexposed control ones was observed in brain (2.5 fold), liver (2.1 fold), kidney medulla (1.9 fold), kidney cortex (1.8 fold) and lung (1.7 fold) cells, while the DNA from lymphocytes and spleen cells was not affected. Conclusion. This study demonstrated that subehronic exposure to sublethal doses of MCs can induce systemicgenotoxicity in mammals, and it affects not only the liver but also other vital organs.
Keywords: DNA damage, comet assay, cyanobacteria, bacterial toxins, rats, inbred F344
Published in DiRROS: 20.02.2024; Views: 236; Downloads: 55
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885. Rheological behaviors of waste polyethylene modified asphalt binder : Statistical analysis of interlaboratory testing resultsDi Wang, Andrea Baliello, Gustavo Pinheiro, Lily D. Poulikakos, Marjan Tušar, Kamilla Vasconcelos, Muhammad Rafiq Kakar, Laurent Porot, Emiliano Pasquini, Gaspare Giancontieri, Chiara Riccardi, Marco Pasetto, Davide Lo Presti, Augusto Cannone Falchetto, 2023, original scientific article Abstract: This article investigated the effect of waste polyethylene (PE) on the modified asphalt binders’ rheological behavior from a statistical point of view. The interlaboratory testing results from the RILEM Technical Committee 279 Valorization of Waste and Secondary Materials for Roads Task Group 1 were used for this purpose. First, an unaged 70/100 penetration graded neat binder was selected as the reference material. Next, a single 5 % content of waste PE additives (PE-pellets and PE-shreds) was mixed with a 95 % neat binder to prepare two PE modified binders. Then, dynamic shear rheometer–based temperature-frequency sweep tests were performed over a wide range of temperatures and frequencies to evaluate the rheological properties of these three binders. Different rheological behaviors were observed in the isochronal plots at high temperatures. Based on a reproducibility precision requirement proposed for phase angle, 28°C was set as the transition temperature across the rheological behaviors. Next, according to the three rheological behaviors defined in a previous study by the authors, statistical analysis was introduced to identify sensitive rheological parameters and determine the thresholds. Results indicate that the phase angle measured above 28°C and 1.59 Hz can be used as a sensitive parameter to discriminate the three rheological behaviors of PE modified binders. The thresholds among different behaviors were also calculated as an example for phase angle measured at the highest common testing temperature of 70°C. Additional experimental evaluations on more types of PE modified binders, especially at intermediate and high temperatures, are recommended to better understand their influence on the rheological behavior of PE modified binders.
Keywords: bitumen, polietilen, reologija, medlaboratorijska primerjava
Published in DiRROS: 20.02.2024; Views: 210; Downloads: 96
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890. Packings in bipartite prisms and hypercubesBoštjan Brešar, Sandi Klavžar, Douglas F. Rall, 2024, original scientific article Abstract: The $2$-packing number $\rho_2(G)$ of a graph $G$ is the cardinality of a largest $2$-packing of $G$ and the open packing number $\rho^{\rm o}(G)$ is the cardinality of a largest open packing of $G$, where an open packing (resp. $2$-packing) is a set of vertices in $G$ no two (closed) neighborhoods of which intersect. It is proved that if $G$ is bipartite, then $\rho^{\rm o}(G\Box K_2) = 2\rho_2(G)$. For hypercubes, the lower bounds $\rho_2(Q_n) \ge 2^{n - \lfloor \log n\rfloor -1}$ and $\rho^{\rm o}(Q_n) \ge 2^{n - \lfloor \log (n-1)\rfloor -1}$ are established. These findings are applied to injective colorings of hypercubes. In particular, it is demonstrated that $Q_9$ is the smallest hypercube which is not perfect injectively colorable. It is also proved that $\gamma_t(Q_{2^k}\times H) = 2^{2^k-k}\gamma_t(H)$, where $H$ is an arbitrary graph with no isolated vertices.
Keywords: 2-packing number, open packing number, bipartite prism, hypercube, injective coloring, total domination number
Published in DiRROS: 19.02.2024; Views: 344; Downloads: 115
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