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831 - 840 / 2000
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Characterization of hFOB 1.19 cell line for studying Zn-based degradable metallic biomaterials
Eva Jablonská, Lucie Mrázková, Jiří Kubásek, Dalibor Vojtěch, Irena Paulin, Tomáš Ruml, Jan Lipov, 2024, original scientific article

Abstract: In vitro testing is the first important step in the development of new biomaterials. The human fetal osteoblast cell line hFOB 1.19 is a very promising cell model; however, there are vast discrepancies in cultivation protocols, especially in the cultivation temperature and the presence of the selection reagent, geneticin (G418). We intended to use hFOB 1.19 for the testing of Zn-based degradable metallic materials. However, the sensitivity of hFOB 1.19 to zinc ions has not yet been studied. Therefore, we compared the toxicity of zinc towards hFOB 1.19 under different conditions and compared it with that of the L929 mouse fibroblast cell line. We also tested the cytotoxicity of three types of Zn-based biomaterials in two types of media. The presence of G418 used as a selection reagent decreased the sensitivity of hFOB 1.19 to Zn2+. hFOB 1.19 cell line was more sensitive to Zn2+ at elevated (restrictive) temperatures. hFOB 1.19 cell line was less sensitive to Zn2+ than L929 cell line (both as ZnCl2 and extracts of alloys). Therefore, the appropriate cultivation conditions of hFOB 1.19 during biomaterial testing should be chosen with caution.
Keywords: zinc degradable materials, in vitro cytotoxicity testing, hFOB 1.19 osteoblasts
Published in DiRROS: 28.02.2024; Views: 244; Downloads: 79
.pdf Full text (1,81 MB)
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836.
Prehranska podpora pri bolnikih z rakom
Karla Berlec, 2023, published scientific conference contribution abstract

Keywords: prehranska podpora, bolniki, onkologija
Published in DiRROS: 27.02.2024; Views: 196; Downloads: 52
.pdf Full text (261,54 KB)

837.
Parenteralni pristopi
Suzana Crljenica, Laura Petrica, Nizra Palamar, Kristina Durić, 2023, published scientific conference contribution abstract

Keywords: parenteralni dostopi, bolniki, onkologija
Published in DiRROS: 27.02.2024; Views: 214; Downloads: 49
.pdf Full text (125,89 KB)

838.
Kadar je zdravstveni delavec bolnik ali bolnikov bližnji
Maja Ebert Moltara, 2023, published scientific conference contribution abstract

Keywords: zdravstveni delavci, bolniki, onkologija
Published in DiRROS: 27.02.2024; Views: 203; Downloads: 49
.pdf Full text (238,57 KB)

839.
Skrb za bolnikove bližnje
Marjana Bernot, 2023, published scientific conference contribution abstract

Keywords: zdravstvena nega, paliativna oskrba, onkologija
Published in DiRROS: 27.02.2024; Views: 232; Downloads: 54
.pdf Full text (166,15 KB)

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