1161. Kartiranje morskega dna in habitatnih tipov ter popis flore in favne na območju predvidene gradnje protipoplavne zaščite mesta Piran : končno poročiloLovrenc Lipej, Ana Fortič, Borut Mavrič, Martina Orlando-Bonaca, Tihomir Makovec, Milijan Šiško, Leon Lojze Zamuda, Domen Trkov, final research report Keywords: Občina Piran, protipoplavna zaščita, kartiranje, habitatni tipi, biološke združbe
Published in DiRROS: 08.09.2025; Views: 305; Downloads: 85
 Full text (6,30 MB) |
1162. Digital PCR genotyping of pepino mosaic virusAmadej Jelenčič, David Dobnik, Alexandra Bogožalec Košir, Nataša Mehle, 2025, independent scientific component part or a chapter in a monograph Abstract: Pepino mosaic virus (PepMV) is a plant pathogen causing significant economic losses in tomato production. Sensitive, reliable, and robust detection methods are crucial for containing the spread of PepMV and reducing its damaging effects. Digital PCR (dPCR) presents several advantages to conventional real-time quantitative PCR (qPCR), including absolute quantification ability, robust quantitative multiplexing capabilities, and straightforward result analysis. Furthermore, dPCR is especially suitable for analysis of complex samples due to its remarkable tolerance to PCR inhibitors, which makes it a promising method for plant virus genotyping. In this chapter, we present two protocols for PepMV genotyping and quantification using one-step reverse transcription digital PCR (RT-dPCR). The first protocol outlines four simplex assays, while the second describes two duplex assays for precise and comprehensive genotyping of PepMV variants.
Keywords: plant viruses, detection, quantification, Pepino mosaic virus, PepMV, digital PCR, dPCR, RT-dPCR, duplex
Published in DiRROS: 08.09.2025; Views: 356; Downloads: 47
Full text (1,54 MB)
This document has many files! More... |
1163. |
1164. |
1165. |
1166. |
1167. |
1168. |
1169. |
1170. |
