1. Sclerosing melanocytic lesions (sclerosing melanomas with nevoid features and sclerosing nevi with pseudomelanomatous features) : an analysis of 90 lesionsBiljana Grčar-Kuzmanov, Emanuela Boštjančič, Juan Antonio Contreras, Jože Pižem, 2018, original scientific article Abstract: Background. Sclerosing melanocytic lesions, which are characterized by either focal or diffuse sclerosis in the dermal component and atypical proliferation of predominantly nevoid melanocytes, remain poorly defined. Our aim was to analyze systematically their morphologic spectrum, especially the distinction between sclerosing melanocytic nevus and sclerosing melanoma, which has not been well documented. Patients and methods. We collected 90 sclerosing melanocytic lesions, occurring in 82 patients (49 male, 33 female; age range from 21 to 89 years). A four probe fluorescent in situ hybridization (FISH) assay was performed in 41 lesions to substantiate the diagnosis of sclerosing melanomas. Results. A prominent full-thickness pagetoid spread of melanocytes was identified in 44 (48%) lesions, and a melanoma in situ adjacent to the sclerosis in 55 (61%) lesions. In the intrasclerotic component, maturation was absent in 40 (44%) and mitotic figures were identified in 18 (20%) lesions. Of the 90 lesions, 26 (29%) were diagnosed morphologically as nevi and 64 (71%) as melanomas (Breslow thickness from 0.4 to 1.8 mm), including 45 (50%) melanomas with an adjacent nevus. A four-probe FISH assay was positive in the sclerotic component in 14 of 25 lesions diagnosed morphologically as melanomas and none of 16 nevi. A sentinel lymph node biopsy was performed for 17 lesions and was negative in all cases. Conclusions. Sclerosing melanocytic lesions form a morphologic spectrum and include both nevi and melanomas. The pathogenesis of sclerosis remains obscure but seems to be induced by melanocytes or an unusual host response in at least a subset of lesions. Keywords: sclerosing melanoma, sclerosing nevus, fibrosis Published in DiRROS: 02.07.2024; Views: 160; Downloads: 164 Full text (1,34 MB) |
2. Detection of apoptotic cells in tumour paraffin sectionsJože Pižem, Andrej Cör, 2003, professional article Abstract: Apoptosis is a distinct form of cell death characterised by specific morphological features and regulated by complex molecular mechanisms. Its deregulation is fundamental for tumour growth and progression and, moreover, anticancer therapies suppress tumour growth mainly by induction of apoptosis. Since the extent of apoptosis in a tumour may have prognostic as well as therapeutic implications, much effort has been invested in developing specificmethods that can be routinely used to detect apoptotic cells in archival formalin fixed paraffin-embedded tissue. Complex molecular pathways are involved in the regulation of apoptosis. Pro-apoptotic signals trigger activation of caspases that specifically cleave target proteins. Cleavage of proteins (caspase substrates) is responsible for morphological changes of apoptotic cells and DNA fragmentation. In the last decade, detection of apoptotic cells in formalin fixed tumour tissue sections has been based mainlyon morphology and characteristic DNA fragmentation. Recently, specific antibodies to activated caspases and cleaved target proteins (including cytokeratin 18, actin and PARP) have been produced that enable accurate detection of apoptosis in paraffin sections. Published in DiRROS: 06.02.2024; Views: 328; Downloads: 76 Full text (170,01 KB) |
3. Survivin - an inhibitor of apoptosis and a new therapeutic target in cancerJože Pižem, Andrej Cör, 2003, professional article Abstract: Survivin is a unique member of the inhibitor of apoptosis (IAP) protein family. It inhibits apoptosis by interfering with post-mitochondrial events during apoptosis, thus blocking activation of caspases. The expression of survivin is among the most tumour specific of all human genes. It is overexpressed in most human cancers but is not detected in most normal tissues. Some molecular mechanisms of survivin upregulation in cancer have been elucidated, including loss of the wild-type p53. Tumours that overexpresssurvivin generally bear a worse prognosis and are associated with resistance to therapy. Its differential expression in caneer versus normal tissues makes survivin detection a useful tool in cancer diagnostics and a promising therapeutic target. Survivin targeting has resulted in increased spontaneous and induced apoptosis and inhibition of tumourgrowth. Some anticaneer drugs currently introduced into clinical practice might well act byinactivaring survivin. Published in DiRROS: 06.02.2024; Views: 312; Downloads: 68 Full text (185,35 KB) |
4. Minimalno invazivni diagnostični pristopi pri primarnih možganskih tumorjih z uporabo prosto cirkulirajočih nukleinskih kislinMarija Skoblar Vidmar, Jernej Mlakar, Uroš Smrdel, Alenka Matjašič, Jože Pižem, Andrej Zupan, 2021, published scientific conference contribution Keywords: tekočinska biopsija, onkološko zdravljenje, možganski tumorji Published in DiRROS: 04.04.2022; Views: 794; Downloads: 300 Full text (123,97 KB) |
5. Priporočila za obravnavo bolnikov z melanomomMarko Hočevar, Primož Strojan, Janja Ocvirk, Barbara Perić, Olga Blatnik, Boštjan Luzar, Jože Pižem, Marko Boc, Tanja Mesti, Marija Ignjatović, Nežka Hribernik, Katarina Barbara Karner, Tomi Bremec, Tanja Planinšek Ručigaj, 2020, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: onkologija, melanomi, maligni melanomi, UV žarki, sončenje, sončne opekline, zaščitne kreme Published in DiRROS: 18.03.2022; Views: 888; Downloads: 299 Full text (739,88 KB) |
6. Ploščatocelični rak kože : priporočila za zdravljenjeBarbara Perić, Olga Blatnik, Boštjan Luzar, Jože Pižem, Janja Ocvirk, Marko Hočevar, Primož Strojan, Tomi Bremec, Martina Reberšek, 2020, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: ploščatocelični rak, rak kože, bolniki, zdravljenje Published in DiRROS: 17.03.2022; Views: 864; Downloads: 434 Full text (842,08 KB) This document has many files! More... |
7. Priporočila za zdravljenje bolnikov s ploščatoceličnim rakom kožeBarbara Perić, Olga Blatnik, Boštjan Luzar, Jože Pižem, Janja Ocvirk, Marko Hočevar, Primož Strojan, Tomi Bremec, Martina Reberšek, 2020, not set Keywords: ploščatocelični rak, rak (medicina), koža, bolniki, zdravljenje Published in DiRROS: 07.04.2021; Views: 1519; Downloads: 475 Full text (191,24 KB) |
8. Smernice & klinična pot za obravnavo bolnikov z melanomomPrimož Strojan, Uroš Ahčan, Marko Snoj, Boštjan Luzar, Jože Pižem, Boris Jančar, Martina Reberšek, Borut Žgavec, Tanja Planinšek Ručigaj, Aleksandra Dugovnik, 2010, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: klasifikacija, smernice, preživetje Published in DiRROS: 28.08.2020; Views: 1825; Downloads: 775 Full text (11,72 MB) |
9. Priporočila za obravnavo bolnikov s karcinomom Merklovih celicMarko Hočevar, Primož Strojan, Janja Ocvirk, Martina Reberšek, Marko Boc, Barbara Perić, Boštjan Luzar, Jože Pižem, Olga Blatnik, Katarina Barbara Karner, Tomi Bremec, 2019, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: rak kože, karcinom Merklovih celic, zdravljenje Published in DiRROS: 27.05.2020; Views: 2019; Downloads: 580 Full text (154,48 KB) |
10. Nova funkcionalnost prenovljenega informacijskega sistema DP ZoraUrška Ivanuš, Maja Primic-Žakelj, Mojca Florjančič, Tine Jerman, Mojca Kuster, Ana Pogačnik, Veronika Kloboves-Prevodnik, Špela Smrkolj, Urška Gašper, Margareta Strojan Fležar, Jože Pižem, Snježana Frković-Grazio, 2017, published scientific conference contribution Keywords: presejalni testi, rak materničnega vratu, informacijski sistem, kakovost Published in DiRROS: 09.04.2020; Views: 2076; Downloads: 553 Full text (99,21 KB) |