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801 - 810 / 2000
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Improving biodiversity in Central and Eastern European gardens needs regionally scaled strategies
Zsófia Varga-Szilay, Arvids Barševskis, Klára Benedek, Danilo Bevk, 2025, original scientific article

Abstract: Amid ongoing urbanisation, gardens are expected to play an increasing role in enhancing urban biodiversity by supplementing green areas and improving landscape connectivity. Biodiversity-friendly gardens also improve human well-being and foster connections between nature and people. To study these benefits, we distributed a questionnaire (n = 5255), and used a scoring system to evaluate gardens’ ecological value (GAR index), gardeners' attitudes (RES index), and pesticide use habits (PES index). We used machine learning to explore how these indices interact and what sociodemographic factors drive them across Central and Eastern Europe (CEE). Our aim was to explore the ecological values of gardens and gardening practices, identifying characteristics that might contribute to building high biodiversity. We found significant variability within and between countries, with Romania scoring low and Czechia high in all indices. Domestic pesticide use was ubiquitous across CEE and largely unaffected by sociodemographic factors. Increased time spent gardening was associated with the highest pesticide use and a greater potential for fostering high biodiversity. Gardeners aged over 55 tended to uphold longstanding conventional practices and thus lowered both PES and GAR index scores. The local differences highlight the need for regionally tailored biodiversity-friendly gardening guidelines instead of standardised regulations across Europe. Effective environmental education and community programs can be developed based on local biodiversity and the three indices we used. These programs should inform gardeners about the environmental and health impacts of pesticides and provide comprehensive biodiversity-related knowledge. This is especially important in CEE, where such initiatives are currently underrepresented.
Keywords: rural-urban gradient, urban ecosystems, environmental consciousness, sustainable gardening, environmental sensitivity, urbanisation
Published in DiRROS: 08.10.2025; Views: 199; Downloads: 91
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From vineyard to genome : optimized enrichment and sequencing of Flavescence dorée phytoplasma from grapevine samples
Zala Kogej Zwitter, Denis Kutnjak, Nataša Mehle, 2025, original scientific article

Abstract: Phytoplasmas are non-culturable obligate intracellular bacteria that cause considerable economic losses in agriculture. Genome sequencing provides crucial insights into their biology and vector dependence. However, genome studies on phytoplasmas are often hampered by their low abundance in naturally infected plants. Propagation in test plants is usually necessary but time-consuming and resource-intensive, especially for quarantine phytoplasmas such as the phytoplasma causing Flavescence dorée (FD), a serious threat to European viticulture. To overcome these challenges, we aimed to develop a protocol for efficient enrichment of phytoplasma DNA directly from field-collected samples, enabling genome sequencing using both Illumina and Oxford Nanopore Technologies platforms. We evaluated six sample preparation protocols that included stepwise enrichment steps to improve phytoplasma genome coverage and assembly quality. The most effective approach combined differential centrifugation, CTAB extraction and removal of CpG-methylated host DNA and resulted in a notable increase in the relative abundance of phytoplasma reads compared to other protocols. Rarefaction analysis of the dataset generated using this protocol demonstrated that the entire phytoplasma genome was covered by reads in a dataset comprising 3 billion nucleotides. We also evaluated and compared de novo phytoplasma genome assemblies generated from short Illumina reads and long nanopore sequencing reads. While Illumina sequencing yielded more accurate assemblies with longer total lengths, the assemblies derived from nanopore sequencing data contained longer individual contigs. This advantage was reflected in hybrid assemblies that combined both technologies, yielding longer phytoplasma contigs than assemblies from Illumina datasets and lower mismatch rates compared to assemblies from nanopore sequencing datasets. A hybrid de novo assembled genome of the Slovenian FD phytoplasma isolate achieved 96% reference genome coverage, with high contiguity and low error rates. This streamlined and accessible protocol enables high-quality genome sequencing of phytoplasma-infected grapevines without the need for propagation in test plants. This facilitates broader phytoplasma research and can potentially be extended to other naturally infected phytoplasma hosts or organisms infected with other non-culturable microbes.
Keywords: plant pathogen, nanopore, grapevine, enrichment, HTS, Flavescence dorée, phytoplasma
Published in DiRROS: 08.10.2025; Views: 217; Downloads: 105
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Complexity of the game connected domination problem
Vesna Iršič Chenoweth, 2025, original scientific article

Abstract: The connected domination game is a variant of the domination game where the played vertices must form a connected subgraph at all stages of the game. In this paper we prove that deciding whether the game connected domination number is smaller than a given integer is PSPACE-complete using log-space reductions for both Dominator- and Staller-start connected domination game.
Keywords: connected domination game, complexity, PSPACE-complete
Published in DiRROS: 06.10.2025; Views: 227; Downloads: 91
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