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372. Multiplex quantification of 19 GM soybean lines using digital PCRAmadej Jelenčič, Dejan Štebih, Tina Demšar, David Dobnik, 2026, independent scientific component part or a chapter in a monograph Abstract: The European Union (EU) imposes strict regulations on the presence of genetically modified (GM) material in food and feed, requiring thorough testing of samples for various GM lines. Although traditional quantitative real-time PCR (qPCR) methods are sensitive and robust, they are not cost-effective for managing large numbers of GM events due to their limited multiplexing capabilities. Conversely, digital PCR (dPCR) is capable of robust quantitative multiplexing in addition to other benefits such as absolute quantification and better tolerance of PCR inhibitors. In this context, we present a protocol for multiplex quantification of 19 GM soybean lines using dPCR as an improvement over the currently used simplex qPCR approach. This method enables simple and robust quantification of common GM soybean lines with a relatively low number of reactions.
Keywords: genetically modified soybean, GMO, multiplexing, food and feed, food regulation, digital PCR (dPCR)
Published in DiRROS: 26.11.2025; Views: 123; Downloads: 37
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373. Strain-controlled superconductivity in epitaxially grown thin films of ▫$1T-TaS_2$▫Yelyzaveta Chernolevska, Anže Mraz, Rok Venturini, Bojan Ambrožič, Tomaž Mertelj, Goran Dražić, Damjan Svetin, Damjan Vengust, Hsin-Chia Ho, Matjaž Spreitzer, Dragan Mihailović, 2025, original scientific article Keywords: transition metal dichalcogenides, thin films, molecular beam epitaxy
Published in DiRROS: 26.11.2025; Views: 142; Downloads: 79
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374. The European Information System on Forest Genetic Resources (EUFGIS): : a transnational tool for genetic conservation and policy supportMichele Bozzano, Valentina Barbiero, Egbert Beuker, Simone Mori, Mari Rusanen, Ivan Scotti, Milko Skofic, Marjana Westergren, 2025, published scientific conference contribution abstract Keywords: forest genetic resources, information system, Europe
Published in DiRROS: 26.11.2025; Views: 110; Downloads: 52
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375. Trans fatty acids in margarines and shortenings in the food supply in SloveniaHelena Abramovič, Rajko Vidrih, Emil Zlatić, Doris Kokalj Sinkovič, Matthias Schreiner, Katja Žmitek, Anita Kušar, Igor Pravst, 2018, original scientific article Published in DiRROS: 26.11.2025; Views: 165; Downloads: 84
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376. Signalling network construction for modelling plant defence responseDragana Miljković, Tjaša Stare, Igor Mozetič, Vid Podpečan, Marko Petek, Kamil Witek, Marina Dermastia, Nada Lavrač, Kristina Gruden, 2012, original scientific article Published in DiRROS: 26.11.2025; Views: 133; Downloads: 64
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377. In vitro evaluation of electrochemotherapy combined with sotorasib in pancreatic carcinoma cell lines harboring distinct kras mutationsTanja Jesenko, Maša Omerzel, Tina Živič, Gregor Serša, Maja Čemažar, 2025, original scientific article Abstract: Pancreatic cancer is among the deadliest malignancies, with limited treatment options and poor prognosis. Novel strategies are therefore urgently needed. Sotorasib, a KRAS G12C-specific inhibitor, offers targeted treatment for a small subset of patients with this mutation. Electrochemotherapy (ECT), which enhances the cytotoxicity of chemotherapeutic agents through electroporation-induced membrane permeabilization, has shown promise in various tumor types, including deep-seated malignancies such as pancreatic cancer. Combining ECT with sotorasib may potentiate antitumor effects in KRAS G12C-mutated pancreatic cancer; however, preclinical data on such combinations are lacking. This proof-of-concept study evaluated the cytotoxic effects of ECT using bleomycin (BLM) or cisplatin (CDDP) in combination with sotorasib in KRAS G12C-mutated MIA PaCa-2 and KRAS G12D-mutated PANC-1 pancreatic cancer cell lines. ECT alone significantly reduced cell viability, particularly in MIA PaCa-2 cells, where electric pulses induced approximately 75% cell death. Combining ECT with sotorasib resulted in an additive effect on KRAS G12C-mutated MIA PaCa-2 cells, though no synergy was observed, likely due to the high intrinsic sensitivity to electric pulses. These results support the potential of combining physical and molecular therapies in a subset of pancreatic cancer patients and lay the groundwork for further in vivo studies to optimize treatment parameters and explore clinical translatability.
Keywords: bleomycin, cisplatin, electrochemotherapy, pancreatic cancer
Published in DiRROS: 26.11.2025; Views: 95; Downloads: 47
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379. Sequencing of chemotherapy in total neoadjuvant treatment for rectal cancer does not predict radiation-induced lymphopeniaMiha Oražem, Vaneja Velenik, Alojz Ihan, 2025, original scientific article Abstract: Radiation-induced lymphopenia (RIL) is associated with an increased risk of death in solid tumors, including rectal cancer. The aim of this study was to determine whether the sequencing of chemotherapy in total neoadjuvant treatment (TNT) for rectal cancer predicts the development of RIL. Patients and methods We analyzed acute hematologic toxicity data from 53 patients who underwent TNT for locally or locoregionally advanced rectal cancer between July 2022 and April 2023. Twenty-eight patients received induction chemotherapy with capecitabine and oxaliplatin [CAPOX], and 25 received consolidation chemotherapy (6 cycles of CAPOX in both groups). The chemoradiation protocol consisted of Volumetric Modulated Arc Therapy with Simultaneous Integrated Boost Radiotherapy (VMAT-SIB RT) up to 48.4 Gy in 22 fractions, concomitantly with capecitabine twice a day (lat. bis in die, BID). The Mann-Whitney U test was performed to compare RIL between the two patient groups. Pelvic bone marrow was contoured as a non-limiting organ-at-risk to assess the received dose, and binary logistic regression was used to determine whether RIL depends on V5Gy~V42Gy or the planning target volume (PTV) size. Results Thirty-four patients (64.2%) developed RIL of any grade, which was not significantly associated with either the induction or consolidation chemotherapy TNT regimen (Wald = 3.159, p = 0.076). No significant differences were found in neutrophil counts or the neutrophil-to-lymphocyte ratio. In the logistic regression model predicting the likelihood of RIL, two variables were statistically significant: V10Gy (Wald = 4.366, p = 0.037) and V30Gy (Wald = 6.084, p = 0.014). These results indicate that V10Gy< 71% and V30Gy< 26.6% may reduce the likelihood of developing RIL. Conclusions In our study, the sequencing of chemotherapy in TNT for rectal cancer did not predict the development of RIL. However, the incidence of RIL may be reduced by applying RT dosimetric constraints to the pelvic bone marrow.
Keywords: radiation-induced lymphopenia, rectal cancer, total neoadjuvant treatment
Published in DiRROS: 26.11.2025; Views: 139; Downloads: 44
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380. Gender impact on quality of life in colorectal cancer survivorsAleksandra Grbič, Majda Čaušević, Sara Brodarič, Mojca Birk, Irena Oblak, 2025, original scientific article Abstract: The aim of the study was to evaluate gender-specific differences in the quality of life (QoL) and late effects among colorectal cancer patients during the first two years after treatment, to inform and improve long-term follow-up care and clinical management strategies. Patients and methods A total of 239 colorectal cancer patients were included, 56% males and 44% females, mostly in the age range 60–69 years. They were treated at the Institute of Oncology Ljubljana, during the time period from 1st September 2023 to 1st May 2024. In addition to demographic data, we included clinical data on disease and outcomes collected using the standardized quality of life questionnaires of European Organization for Research and Treatment of Cancer (EORTC) named EORTC QLQ-30 and EORTC QLQ-CR29 for colorectal cancer, respectively. Results Females were more likely to experience emotional problems (p = 0.002), higher levels of fatigue (p < 0.001), insomnia (p = 0.015), nausea and vomiting (p = 0.007), which may also be associated with poorer appetite in females. Males reported better body image than female (p = 0.047), lower levels of anxiety (p = 0.029), less frequently reported perceived weight loss or gain (p = 0.010). Male reported more stool frequency (p = 0.045), and also had more sever dysuria compared to female (p = 0.008). Conclusions The results provide the opportunity to improve the clinical management of long-term follow-up and care planning, taking into consideration the gender-specific needs of colorectal cancer survivors.
Keywords: quality of life, late effects, colorectal cancer
Published in DiRROS: 26.11.2025; Views: 149; Downloads: 44
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