| Naslov: | Luciferase-induced immune activation prevents orthotopic K7M2luc osteosarcoma establishment in BALB/c mice |
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| Avtorji: | ID Kupčič, Saša (Avtor)
ID Kamenšek, Urška (Avtor)
ID Čemažar, Maja (Avtor)
ID Lampreht Tratar, Urša (Avtor) |
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| Datoteke: |  PDF - Predstavitvena datoteka, prenos (1,95 MB)
MD5: C67A2A127BE390E159A54D68D34560C7
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| Jezik: | Angleški jezik |
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| Tipologija: | 1.01 - Izvirni znanstveni članek |
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| Organizacija: |  OI - Onkološki inštitut Ljubljana
 DRO - Društvo radiologije in onkologije
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| Povzetek: | Background. Use of murine orthotopic tumor models dictates utilization of luciferase-expressing cells for in vivo biolu-minescence imaging. However, luciferase may trigger immune responses that impair tumor establishment.Materials and methods. We transduced K7M2 cells wild-type (wt) with luciferase gene and evaluated their growth in vitro. We then implanted cells orthotopically in BALB/c mice and upon unsuccessful tumor establishment assessed systemical and local mechanism for tumor growth suppression.Results. All investigated cell lines had a comparative doubling time in vitro at 24 and 48h. Despite that, K7M2luc E5 differed from K7M2luc A7 and K7M2 wt by having a much higher proliferation rate at 72h (35x, 15x and 17x). In vivo, both luciferase-expressing cell lines failed to establish tumors, whereas K7M2 wt had a 100% success rate. To investi-gate the underlying mechanism, we assessed systemic and local cell-mediated immunity. Mice in groups K7M2luc A7 and K7M2luc E5 had a higher fold change (5 ± 0.8 and 6 ± 1.05) of GrB secreting splenocytes than naïve mice (1 ± 0.2). In the bone microenvironment, luciferase groups exhibited elevated numbers of GrB-secreting cells and CD8+T cells relative to K7M2 wt and naïve mice.Conclusions. These findings indicate that luciferase activates cytotoxic T-cell responses capable of eliminating im-planted cells. Tumor establishment was restored in immunodeficient mice lacking CD8+ cells, confirming the immune-mediated rejection. |
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| Ključne besede: | mouse orthotopic tumor, luciferase, bioluminescence, osteosarcoma |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Poslano v recenzijo: | 17.08.2026 |
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| Datum sprejetja članka: | 21.08.2026 |
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| Datum objave: | 01.09.2026 |
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| Založnik: | Association of Radiology and Oncology |
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| Leto izida: | 2026 |
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| Št. strani: | str. 402-415 |
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| Številčenje: | Vol. 60, no. 3 |
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| Izvor: | Ljubljana |
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| PID: | 20.500.12556/DiRROS-32354  |
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| UDK: | 602.6/.7 |
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| ISSN pri članku: | 1318-2099 |
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| DOI: | 10.2478/raon-2026-0049 |
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| COBISS.SI-ID: | 290269699 |
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| Avtorske pravice: | by Authors |
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| Datum objave v DiRROS: | 09.09.2026 |
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| Število ogledov: | 60 |
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| Število prenosov: | 39 |
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| Metapodatki: |    |
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