| Naslov: | In vitro genotoxicity assessment of graphene quantum dots using a 3D HepG2 model : version v1 |
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| Avtorji: | ID Durmišević, Irma (Avtor)
ID Haverić, Anja (Avtor)
ID Kološa, Katja (Avtor)
ID Žegura, Bojana (Avtor) |
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| Datoteke: |  URL - Izvorni URL, za dostop obiščite https://zenodo.org/records/18415123
 DOCX - Raziskovalni podatki, prenos (26,18 KB)
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| Jezik: | Angleški jezik |
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| Tipologija: | 2.20 - Zaključena znanstvena zbirka raziskovalnih podatkov |
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| Organizacija: |  NIB - Nacionalni inštitut za biologijo
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| Povzetek: | Graphene quantum dots (GQDs) are nano-sized fragments of graphene sheets, with great potential for applications in optics, electrochemistry, and biomedicine, including drug delivery. In this study, two types of GQDs were investigated: green-emitting (G-GQD) and blue-emitting (B-GQD) and their genotoxic potential was examined on the protein level. Flow cytometric analysis of γH2AX, a marker of DNA double-strand breaks, and phosphorylated histone H3 (p-H3), a marker of aneugenic activity, was performed in HepG2 spheroids after 24 hours of exposure to G-GQDs and B-GQDs. HepG2 spheroids were exposed to graded concentrations of B- and G-GQD (12.5, 25, 50, and 100 μg/mL) for 24 hours, respectively. Spheroids were dissociated into single-cell suspensions, washed twice with 1x PBS and fixed in 4% PFA. For flow cytometric analysis, cells were labelled with anti-H2AX pS139 antibody and anti-histone H3 pS128 antibody. REA-APC and REA-PE controls were applied to exclude non-specific antibody binding. For each sample, 10,000 events were acquired using a MACSQuant Analyzer 10 flow cytometer and MACSQuantify™ software (Miltenyi Biotech, Germany). Raw data were exported from the MACSQuantify software and analysed with FlowJo V10 software (Becton Dickinson, New Jersey, USA). |
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| Ključne besede: | nanomaterials, graphene quantum dots, HepG2 spheroids, toxicity |
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| Status publikacije: | Objavljeno |
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| Verzija publikacije: | Objavljena publikacija |
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| Datum objave: | 29.01.2026 |
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| Kraj izida: | Genève |
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| Kraj izvedbe: | Genève |
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| Založnik: | Zenodo |
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| Leto izida: | 2026 |
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| Leto izvedbe: | 2026 |
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| Št. strani: | 1 spletni vir |
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| PID: | 20.500.12556/DiRROS-31433  |
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| UDK: | 579 |
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| DOI: | 10.5281/zenodo.18415123 |
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| COBISS.SI-ID: | 280617475 |
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| Opomba: | Nasl. z. nasl. zaslona;
Opis vira z dne 5. 6. 2026;
Soavtorji: Haverić, Anja; Kološa, Katja; Zegura, Bojana;
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| Datum objave v DiRROS: | 31.07.2026 |
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| Število ogledov: | 40 |
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| Število prenosov: | 28 |
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| Metapodatki: |    |
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